Abstract：
:A simple, sensitive and specific colourimetric hybridisation method for the detection of HCMV DNA in clinical specimens is described. This method combines a PCR assay with a sensitive sandwich hybridisation assay. It relies on the use of a specific capture probe linked covalently to polystyrene microplates and a specific polybiotinylated detection probe. Amplified DNA fragments, sandwiched between these two probes, are detected by an enzymatic colour reaction. This PCR-based colourimetric hybridisation method was compared with other known HCMV detection methods. Clinical specimens (n = 145, corresponding to 106 patients) were tested by both a nested PCR assay and this colourimetric hybridisation method; and by either the culture method or the pp65 antigenaemia test depending on the type of sample used. The results showed that the PCR-based hybridisation method has a specificity similar to tissue culture, known as the conventional gold standard method, and could be used for the examination of the clinical specimens.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Mansy F,Brancart F,Liesnard C,Bollen A,Godfroid Edoi
10.1016/s0166-0934(99)00023-3subject
Has Abstractpub_date
1999-07-01 00:00:00pages
113-22issue
2eissn
0166-0934issn
1879-0984pii
S0166093499000233journal_volume
80pub_type
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