Abstract：
:A simple sequencing-based assay is described for genotyping of hepatitis C virus (HCV). RT-PCR was employed to amplify a 237-nucleotide-long fragment from the 5' untranslated region (UTR) of the genome using one biotinylated and one normal primer. Subsequent to capture of the PCR products on streptavidin-coated beads, single-stranded DNA separation, and hybridization of sequencing primer, Pyrosequencing was performed. The genotype of 98 samples out of which 77 samples were from American veterans and 21 samples were from Iran was determined. The samples from the American veterans contained six different subtypes, while five subtypes were found in Iranian samples. For rapid population-specific HCV subtyping, a multiplex assay was developed. This study demonstrates the suitability of this technology for low-cost, high throughput and accurate microbial genotyping.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Elahi E,Pourmand N,Chaung R,Rofoogaran A,Boisver J,Samimi-Rad K,Davis RW,Ronaghi Mdoi
10.1016/s0166-0934(03)00068-5subject
Has Abstractpub_date
2003-05-01 00:00:00pages
171-6issue
2eissn
0166-0934issn
1879-0984pii
S0166093403000685journal_volume
109pub_type
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