Abstract:
:Indicator cell lines are useful biological tools for monitoring virus infection. In order to monitor infection with bovine immunodeficiency virus (BIV) in vitro, an indicator cell line derived from baby hamster kidney cells which contains integrated copies of an enhanced green fluorescent protein gene driven by the BIV long terminal repeat was constructed. The BIV indicator cell line, designated BIVE, can detect BIV infection more easily and effectively than the established method, which involves the observation of cell cytopathic effects. Furthermore, viral titration using an assay based on the indicator cells is 100 times more sensitive than the assay based on cytopathic effect. The finding that BIV can infect the hamster cell line expands the known host range of BIV in vitro. The BIV indicator cell line could also be used for the evaluation of the inhibitory effect of antiviral agents. The fusion inhibition effect of the heptad repeat 2 region of the BIV envelope protein could also be quantified.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Yao X,Su Y,Liu C,Tan J,Liu L,Geng YQ,Qiao WTdoi
10.1016/j.jviromet.2009.07.007subject
Has Abstractpub_date
2010-01-01 00:00:00pages
25-30issue
1eissn
0166-0934issn
1879-0984pii
S0166-0934(09)00334-6journal_volume
163pub_type
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journal_title:Journal of virological methods
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更新日期:1996-06-01 00:00:00
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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pub_type: 临床试验,杂志文章
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
pub_type: 杂志文章
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更新日期:1983-09-01 00:00:00
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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更新日期:1988-09-01 00:00:00