Abstract：
:Cutting edge immune monitoring techniques increasingly measure multiple functional outputs for various cell types, such as intracellular cytokine staining (ICS) assays that measure cytokines expressed by T cells. To date, however, there is no precise method to measure virus-specific cytokine production by both T cells as well as NK cells in the same well, which is important to a greater extent given recent identification of NK cells expressing a memory phenotype. This study describes an adaptable and efficient ICS assay platform that can be used to detect antigen-driven cytokine production by human T cells and NK cells, termed "viral ICS". Importantly, this assay uses limited amount of cryopreserved PBMCs along with autologous heat-inactivated serum, thereby allowing for this assay to be performed when sample is scarce as well as geographically distant from the laboratory. Compared to a standard ICS assay that detects antigen-specific T cell cytokine expression alone, the viral ICS assay is comparable in terms of both HIV-specific CD4 and CD8T cell cytokine response rates and magnitude of response, with the added advantage of ability to detect virus-specific NK cell responses.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Pattacini L,Murnane PM,Fluharty TR,Katabira E,De Rosa SC,Baeten JM,Lund JMdoi
10.1016/j.jviromet.2014.01.003subject
Has Abstractpub_date
2014-04-01 00:00:00pages
17-24eissn
0166-0934issn
1879-0984pii
S0166-0934(14)00013-5journal_volume
199pub_type
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