Abstract:
:This paper describes the cloning, sequencing and bacterial expression of the N protein of the Rift Valley fever virus (RVFV) ZIM688/78 isolate and its evaluation in indirect ELISAs (I-ELISA) for the detection of IgM and IgG antibodies in human and sheep sera. Sera used for the evaluation were from 106 laboratory workers immunised with an inactivated RVF vaccine, 16 RVF patients, 168 serial bleeds from 8 sheep experimentally infected with wild type RVFV and 210 serial bleeds from 10 sheep vaccinated with the live attenuated Smithburn RVFV strain. All human and animal sera that tested positive in the virus neutralisation test were also positive in the IgG I-ELISA. There was a high correlation (R2=0.8571) between virus neutralising titres and IgG I-ELISA readings in human vaccinees. In experimentally infected sheep IgG antibodies were detected from day 4 to 5 post-infection onwards and IgM antibodies from day 3 to 4. The IgG I-ELISA was more sensitive than virus neutralisation and haemagglutination-inhibition tests in detecting the early immune response in experimentally infected sheep. The I-ELISAs demonstrated that the IgG and IgM response to the Smithburn vaccine strain was slower and the levels of antibodies induced markedly lower than to wild type RVFV infection.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Jansen van Vuren P,Potgieter AC,Paweska JT,van Dijk AAdoi
10.1016/j.jviromet.2006.11.005subject
Has Abstractpub_date
2007-03-01 00:00:00pages
106-14issue
1-2eissn
0166-0934issn
1879-0984pii
S0166-0934(06)00390-9journal_volume
140pub_type
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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