Multiple primer pairs polymerase chain reaction for the detection of human papillomavirus types.

Abstract:

:A polymerase chain reaction (PCR) based on the use of multiple primers enabling the simultaneous detection of HPV-6b, -11, -16 and -18 in a single tube reaction was developed and validated on cervico-vaginal specimens, including tissues embedded in paraffin. This PCR setting proved to be specific and sensitive, allowing the detection of as few as 200 viral particles per specimen.

journal_name

J Virol Methods

authors

Anceschi MM,Falcinelli C,Pieretti M,Cosmi EV

doi

10.1016/0166-0934(90)90087-v

subject

Has Abstract

pub_date

1990-04-01 00:00:00

pages

59-65

issue

1

eissn

0166-0934

issn

1879-0984

pii

0166-0934(90)90087-V

journal_volume

28

pub_type

杂志文章
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  • Primary diagnosis of foot-and-mouth disease by reverse transcription polymerase chain reaction.

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