当前位置： SCI文献检索 > JOURNAL OF VIROLOGICAL METHODS期刊下所有文献 > Design and heterologous expression of dengue virus envelope protein (E) peptides and their use for serological diagnosis.

Design and heterologous expression of dengue virus envelope protein (E) peptides and their use for serological diagnosis.

Abstract：

:Viruses belonging to the Flaviviridae family are found and distributed in most of the tropical and sub-tropical regions of the world. The genus has more than 56 members, most of which cause clinical symptoms in humans. The clinical diagnosis of dengue requires laboratory confirmation because of the similarity of symptoms with a series of other acute fevers and the primary use antibodies or antigens for detection. In this work, peptides E(1) and E(2) of the envelope protein (E) of the dengue virus were mapped using bioinformatics methods. These peptides were then expressed in a prokaryotic system and purified. An indirect ELISA for antibodies IgG and IgM from laboratory samples previously characterised was then used with the peptides to detect anti-dengue antibodies. For IgG using the peptide E(1), the sensitivity of the indirect ELISA was 88.3% and the specificity was 56%; using the peptide E(2), the sensitivity was 90% and the specificity was 59%; and using a combination of both peptides, the sensitivity was 93.3% and the specificity was 78%. For IgM using the peptide E(1), the sensitivity was 88% and the specificity was 66%; using the peptide E(2), the sensitivity was 88% and the specificity was 69%; and when used in combination, the peptides E(1)/E(2) demonstrated a sensitivity of 90% and a specificity of 86%. These results indicate that the use of the E(1) and E(2) peptides of the E protein are an alternative for serological diagnosis of dengue fever.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Honda ER,Zanchi F,Rios K,Lira E,DeusileneVieira,da Silva LH,De Paula SO

doi

10.1016/j.jviromet.2012.08.006

subject

Has Abstract

pub_date

2012-12-01 00:00:00

pages

55-61

issue

1-2

eissn

0166-0934

issn

1879-0984

pii

S0166-0934(12)00285-6

journal_volume

186

pub_type

杂志文章

相关文献

JOURNAL OF VIROLOGICAL METHODS文献大全
  • Rapid detection of human parvovirus B19 DNA by dot-hybridization and the polymerase chain reaction.

    abstract：:The results of a comparison of three DNA-detection methods for human parvovirus B19 DNA are described. The sensitivity of detection of virus from hybridization assays using 32P-radiolabeled DNA and RNA probes was compared with a method for enzymatically amplifying specific target DNA sequences (polymerase chain reacti...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(89)90085-2

    authors： Salimans MM,Holsappel S,van de Rijke FM,Jiwa NM,Raap AK,Weiland HT

    更新日期：1989-01-01 00:00:00

  • Mapping of epitopes of VP2 protein of chicken anemia virus using monoclonal antibodies.

    abstract：:To map the epitopes of VP2 protein of chicken anemia virus (CAV), VP2 was expressed as a fusion protein in Escherichia coli BL21 (DE3). The Western blot demonstrated that recombinant VP2 protein could be recognized by sera of chickens infected with CAV. Female BALB/c mice were immunized with purified recombinant VP2 p...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2007.03.016

    authors： Wang X,Gao H,Gao Y,Fu C,Wang Z,Lu G,Cheng Y,Wang X

    更新日期：2007-08-01 00:00:00

  • Detection and quantitation of HBV DNA in the WHO International Standard for HIV-1 RNA (NIBSC code: 97/656).

    abstract：:Nucleic-acid amplification technology (NAT) assays have been implemented for HCV and HIV-1 in the United States, and many parts of Europe, Australia and Asia. Nucleic acid detection assays utilize many different technologies, and the WHO International Standards for nucleic acid tests are widely used to compare them. C...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2004.01.021

    authors： Shyamala V,Cottrell J,Arcangel P,Madriaga D,Linnen J,Phelps B,Chien D

    更新日期：2004-06-01 00:00:00

  • A screening assay for antiviral compounds targeted to the HIV-1 gp41 core structure using a conformation-specific monoclonal antibody.

    abstract：:The human immunodeficiency virus type 1 (HIV-1) gp41 plays an important role in membrane fusion between viruses and target cells. The gp41 ectodomain contains two heptad repeat regions adjacent to the N and C-termini. Peptides derived from these two regions, designated N and C-peptides, are potent inhibitors of HIV-1 ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(99)00041-5

    authors： Jiang S,Lin K,Zhang L,Debnath AK

    更新日期：1999-06-01 00:00:00

  • A quantitative duplex PCR technique for measuring amounts of cell-associated Marek's disease virus: differences in two populations of lymphoma cells.

    abstract：:A duplex polymerase chain reaction (PCR) was developed to measure Marek's disease virus (MDV) load in two subpopulations of Marek's disease (MD) lymphoma cells from chickens. PCR primers were designed using the sequence of the MDV-ICP4 gene and the chicken IFNgamma gene. Each set of primers was present in the same rea...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(99)00075-0

    authors： Burgess SC,Davison TF

    更新日期：1999-09-01 00:00:00

  • Human cytomegalovirus DNA kinetics using a novel HCMV DNA quantitative assay in white blood cells of immunocompromised patients under Ganciclovir therapy.

    abstract：:The clearance of human cytomegalovirus (HCMV) was evaluated in infected patients under Ganciclovir (GCV) treatment, using a novel HCMV DNA quantitation assay (HCMV DNA hybrid capture system, Murex Diagnostics). Peripheral white blood cells (WBC) from whole blood specimens of seven AIDS patients, three kidney and two a...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(97)02180-0

    authors： Payan C,Veal N,Sarol L,Villarmé M,Ngohou C,Riberi P,François S,Ifrah N,Loison J,Chennebault JM,Pichard E,Kouyoumdjian S,Lunel F

    更新日期：1997-04-01 00:00:00

  • Comparison of monoplex and duplex RT-PCR assays for the detection of measles virus.

    abstract：:Rapid and accurate detection of measles virus is important for case diagnosis and public health management. This study compared the performance of two monoplex RT-PCR reactions targeting the H and N genes to a duplex RT-PCR targeting both genes simultaneously. The duplex simplified processing without compromising assa...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2016.11.003

    authors： Binkhamis K,Gillis H,Lafreniere JD,Hiebert J,Mendoza L,Pettipas J,Severini A,Hatchette TF,LeBlanc JJ

    更新日期：2017-01-01 00:00:00

  • Strategies for detection of transfusion-transmitted viruses eluding identification by conventional serologic tests. II. Detection of host DNA in human plasmas with elevated alanine aminotransferase.

    abstract：:As a prelude to the development of nucleic acid probes specific for non-A, non-B hepatitis virus(es) (NANBV), plasmas with alanine aminotransferase levels greater than or equal to 110 IU were assayed for DNA by a radioimmunoassay. Approximately 50% of such plasmas are expected to contain NANBV. One-hundred and seventy...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(84)90042-9

    authors： Neurath AR,Strick N,Miller K,Waldman AA

    更新日期：1984-02-01 00:00:00

  • A method for the purification of rotaviruses and adenoviruses from faeces.

    abstract：:Rotaviruses and non-cultivable enteric adenoviruses were purified from large volumes of faecal extracts using polyethylene glycol precipitation, centrifugation through 45% w/v sucrose and segregation by density in a cesium chloride density gradient. Yields were high and the preparations were pure enough to use in the ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(82)90059-3

    authors： Beards GM

    更新日期：1982-08-01 00:00:00

  • Direct RT-PCR method for detecting two chrysanthemum viroids using minimal amounts of plant tissue.

    abstract：:A direct reverse transcription-polymerase chain reaction (RT-PCR) method for detecting the chrysanthemum stunt viroid (CSVd) and chrysanthemum chlorotic mottle viroid (CChMVd) to screen for a viroid-free chrysanthemum plant at a small plant size was established and named microtissue direct RT-PCR. A razor or syringe n...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2005.07.001

    authors： Hosokawa M,Matsushita Y,Uchida H,Yazawa S

    更新日期：2006-01-01 00:00:00

  • An immunochromatography assay for rapid antemortem diagnosis of dogs suspected to have canine distemper.

    abstract：:A new assay was developed for rapid and antemortem diagnosis of canine distemper (CD). This immunochromatography (IC)-based assay, which employs two monoclonal anti-CDV antibodies, was compared with nested PCR. When serial dilutions of purified CDV were tested, the CDV detection limits of the nested PCR and IC assays ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2007.09.006

    authors： An DJ,Kim TY,Song DS,Kang BK,Park BK

    更新日期：2008-02-01 00:00:00

  • Development and evaluation of a reverse transcription loop-mediated isothermal amplification assay for H3N8 equine influenza virus.

    abstract：:Reverse transcription loop-mediated isothermal amplification (RT-LAMP) was applied to the detection of equine influenza virus (EIV). Because equine influenza is caused currently by EIV of the H3H8 subtype, the RT-LAMP primer set was designed to target the hemagglutinin gene of this subtype. The detection limit of the ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2011.07.015

    authors： Nemoto M,Yamanaka T,Bannai H,Tsujimura K,Kondo T,Matsumura T

    更新日期：2011-12-01 00:00:00

  • A biotin-avidin-amplified inhibition enzyme immunoassay for detection of CMV antibodies in human serum.

    abstract：:In this inhibition immunoassay undiluted serum reacts in solution with crude cellular CMV antigen in wells of microtestplates coated with hyperimmune CMV-reactive monkey IgG. CMV antibodies in the serum under test block (completely or partial) the fixation of antigen to the capture layer. Unblocked antigenic activity ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(87)90004-8

    authors： Nielsen CM,Hansen K,Andersen HM,Gerstoft J,Vestergaard BF

    更新日期：1987-06-01 00:00:00

  • Genetic variations in a well conserved 5'-untranslated region of hepatitis C virus genome isolated in Pakistan.

    abstract：:The diversity and extent of sequence variations between hepatitis C virus (HCV) isolates from Pakistan were studied and the probable effects of these variations were assessed on secondary viral structures. Sequencing and phylogenetic analysis was performed on 33 samples, of which 25 were typed as genotype 3 by RFLP (r...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2009.04.007

    authors： Yasmeen A,Siddiqui AA,Hamid S,Sultana T,Jafri W,Persson MA

    更新日期：2009-09-01 00:00:00

  • Enhanced and efficient detection of virus-driven cytokine expression by human NK and T cells.

    abstract：:Cutting edge immune monitoring techniques increasingly measure multiple functional outputs for various cell types, such as intracellular cytokine staining (ICS) assays that measure cytokines expressed by T cells. To date, however, there is no precise method to measure virus-specific cytokine production by both T cells...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2014.01.003

    authors： Pattacini L,Murnane PM,Fluharty TR,Katabira E,De Rosa SC,Baeten JM,Lund JM

    更新日期：2014-04-01 00:00:00

  • A generic real-time TaqMan assay for specific detection of lapinized Chinese vaccines against classical swine fever.

    abstract：:Classical swine fever (CSF) is a highly contagious disease, causing severe economic losses in the pig industry worldwide. Vaccination of pigs with lapinized Chinese vaccines is still practised in some regions of the world, where the virus is enzootic, in order to prevent and control the disease. However, a single real...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2011.05.003

    authors： Liu L,Xia H,Everett H,Sosan O,Crooke H,Meindl-Böhmer A,Qiu HJ,Moennig V,Belák S,Widén F

    更新日期：2011-08-01 00:00:00

  • H5N1 Oseltamivir-resistance detection by real-time PCR using two high sensitivity labeled TaqMan probes.

    abstract：:A single amino acid substitution, from histidine to tyrosine at position 274 of the neuraminidase gene has converted Oseltamivir sensitive H5N1 influenza A virus into a resistant strain. Currently, Oseltamivir is being stockpiled in many countries potentially affected by the influenza A virus subtype H5N1 epidemic. To...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2006.09.007

    authors： Chutinimitkul S,Suwannakarn K,Chieochansin T,Mai le Q,Damrongwatanapokin S,Chaisingh A,Amonsin A,Landt O,Songserm T,Theamboonlers A,Poovorawan Y

    更新日期：2007-01-01 00:00:00

  • Factors contributing to deletion within Mungbean yellow mosaic virus partial dimers in binary vectors used for agroinoculation.

    abstract：:Mungbean yellow mosaic virus-Vigna (MYMV) sequences cloned as partial dimers within the T-DNA of a binary vector were deleted at a high frequency upon conjugal mobilization from Escherichia coli into Agrobacterium tumefaciens. This deletion involving the genome-length viral DNA did not occur when the binary plasmid wa...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2006.06.002

    authors： Shivaprasad PV,Thomas M,Balamani V,Biswas D,Vanitharani R,Karthikeyan AS,Veluthambi K

    更新日期：2006-10-01 00:00:00

  • A non-phenol-chloroform extraction of double-stranded RNA from plant and fungal tissues.

    abstract：:Double-stranded RNA (dsRNA) molecules of viruses are found in nature at a very high frequency. Their detection in plants and fungi has been carried out with difficulty due to the complicated dsRNA extraction techniques used commonly which includes phenol-chloroform extractions. In this study, an extraction method for ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2008.06.001

    authors： Balijja A,Kvarnheden A,Turchetti T

    更新日期：2008-09-01 00:00:00

  • Simple serological diagnosis of arboviruses: a simplified diluent system for Japanese encephalitis virus hemagglutination-inhibition tests using formalinized chick erythrocytes.

    abstract：:Formalinized chick erythrocytes agglutinated with Japanese encephalitis virus antigens over a wider range of pH (6.0-7.2) than fresh erythrocytes (pH 6.4-6.8). The virus antigens were unstable below pH 7.0 and therefore the use of the formalinized erythrocytes made it possible for a one-diluent system of the hemagglut...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(80)90026-9

    authors： Ito H,Iwasa S

    更新日期：1980-01-01 00:00:00

  • Immunodiagnosis of Prune dwarf virus using antiserum produced to its recombinant coat protein.

    abstract：:Certification represents the first line of defense against fruit tree viruses. For certification or surveys dealing with large number of samples, ELISA is still considered the technique of choice and requires a continuous supply of good quality antibodies. Prune dwarf virus (PDV) is among the major viruses affecting s...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2004.05.013

    authors： Abou-Jawdah Y,Sobh H,Cordahi N,Kawtharani H,Nemer G,Maxwell DP,Nakhla MK

    更新日期：2004-10-01 00:00:00

  • A colorimetric reverse transcriptase assay optimized for Moloney murine leukemia virus, and its use for characterization of reverse transcriptases of unknown identity.

    abstract：:A non-radioactive reverse transcriptase (RT) assay, reported as useful for lentivirus RTs, was optimized for the measurement of Moloney murine leukemia virus (MMuLV) RT. The optimized assay could detect 0.3 microU of MMuLV RT. The specificities of the MMuLV and lenti RT assays were demonstrated using the RTs of human ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(98)00091-3

    authors： Malmsten A,Ekstrand DH,Akerblom L,Gronowitz JS,Källander CF,Bendinelli M,Matteucci D

    更新日期：1998-11-01 00:00:00

  • Development of SYBR Green based real time PCR assay for detection of monodon baculovirus in Penaeus monodon.

    abstract：:Shrimp farming is one of the most important aquaculture activities. Expansion and intensification of shrimp farming has been accompanied with the outbreak of diseases, which threaten the development and sustainability of the industry. Viral diseases are the major challenges faced by shrimp farming industries. The prev...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2014.05.006

    authors： Ramesh Kumar D,Sanjuktha M,Rajan JJ,Ananda Bharathi R,Santiago TC,Alavandi SV,Poornima M

    更新日期：2014-09-01 00:00:00

  • Selection and characterization of single-chain recombinant antibodies against infectious haematopoietic necrosis virus from mouse phage display library.

    abstract：:Six single-chain fragment variable (scFv) antibodies against infectious haematopoietic necrosis virus (IHNV) were selected from an antibody phage display library by phage display technology. The soluble scFv antibodies showed a molecular weight 32kDa by Western blot. Dot blot analysis revealed that the six scFv antibo...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2014.04.008

    authors： Liu H,Zheng X,Shi X,Yu L,Jia P,Wang J,He J,Lan W,Liu H,Wu Z

    更新日期：2014-09-01 00:00:00

  • Cloning and expression of two fragments of the S gene of canine coronavirus type I.

    abstract：:Two fragments, S66 and S55, of the S glycoprotein of the newly identified canine coronavirus type I (CCoV type I), were expressed in a procariotic system. The purified recombinant proteins of 350 and 366 amino acids in length, respectively, were employed to develop an enzyme-linked immunosorbent assay (ELISA) for the ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2003.12.003

    authors： Pratelli A,Elia G,Decaro N,Tola S,Tinelli A,Martella V,Rocca S,Tempesta M,Buonavoglia C

    更新日期：2004-04-01 00:00:00

  • The selection and characterization of human monoclonal antibodies to human cytomegalovirus.

    abstract：:This communication describes the application of Epstein-Barr virus lymphocyte transformation technology to the production of human monoclonal antibodies specific for human cytomegalovirus. A group of such IgG antibodies have been characterized in terms of subclass, light-chain composition, specificity for particular v...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(86)90003-0

    authors： Redmond MJ,Leyritz-Wills M,Winger L,Scraba DG

    更新日期：1986-08-01 00:00:00

  • A combined genotypic and phenotypic human immunodeficiency virus type 1 recombinant virus assay for the reverse transcriptase and integrase genes.

    abstract：:With the approval of the first HIV-1 integrase inhibitor raltegravir and a second one in phase III clinical development (elvitegravir), genotypic and phenotypic resistance assays are required to guide antiretroviral therapy and to investigate treatment failure. In this study, a genotypic and phenotypic recombinant vir...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2009.06.015

    authors： Van Baelen K,Rondelez E,Van Eygen V,Ariën K,Clynhens M,Van den Zegel P,Winters B,Stuyver LJ

    更新日期：2009-11-01 00:00:00

  • Rapid rate-kinetic turbidometric assay for quantitation of viral complement-fixing antibodies.

    abstract：:A rapid rate-kinetic turbidometric assay for the quantitation of viral complement fixing antibodies has been developed, using adenovirus as a model. The procedure is based on the turbidometric quantitation of intact sheep erythrocytes and measures the rate of hemolysis (change in absorbance at 640 nm/min/), at maximum...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(85)90003-5

    authors： Fulton RE,Dininno VL

    更新日期：1985-10-01 00:00:00

  • Quantitation of hepatitis B virus DNA (HBV DNA) in serum using the spot hybridization technique and scintillation counting.

    abstract：:The simple spot hybridization technique to detect HBV DNA in serum was modified to concentrate Dane particles by pelleting. This minimised interference by serum components and allowed larger volumes of sera (up to 500 microliters) to be used in serial dilution on borderline positive samples and increased the efficienc...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(85)90136-3

    authors： Fagan EA,Guarner P,Perera SD,Trowbridge R,Rolando N,Davison F,Williams R

    更新日期：1985-12-01 00:00:00

  • Rapid acquisition of entire DNA polymerase gene of a novel herpesvirus from green turtle fibropapilloma by a genomic walking technique.

    abstract：:A 4837-bp sequence of a newfound green turtle herpesvirus (GTHV), implicated in the etiology of green turtle fibropapilloma, was obtained from tumor tissues of a green turtle with fibropapilloma using a genomic walking method based on restriction enzyme digestion, self-ligation and inverse polymerase chain reaction (I...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(00)00267-6

    authors： Yu Q,Hu N,Lu Y,Nerurkar VR,Yanagihara R

    更新日期：2001-02-01 00:00:00