Abstract：
:The genus Caulimovirus consists of several distinct virus species with a double-stranded DNA genome that infect diverse plant species. A comparative analysis of the sequences of known Caulimovirus species revealed two regions that are conserved in all Caulimovirus species with the exception of Strawberry vein banding virus. Degenerate primers based on these two regions were designed and tested in a polymerase chain reaction-based assay for broad spectrum detection of members of this genus. Cauliflower mosaic virus, Figwort mosaic virus and three distinct caulimoviruses associated with dahlia (Dahlia variabilis) were used to show the utility of this test in detecting diverse caulimoviruses. The primer pair gave an amplicon of expected size (840bp). Amplicons from each virus were cloned and sequenced to verify their identity. The primer pair and the PCR assay provide approach for the broad spectrum detection of several members of the genus Caulimovirus.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Pappu HR,Druffel KLdoi
10.1016/j.jviromet.2008.11.014subject
Has Abstractpub_date
2009-04-01 00:00:00pages
102-4issue
1eissn
0166-0934issn
1879-0984pii
S0166-0934(08)00424-2journal_volume
157pub_type
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