Abstract：
:Four IgG(1kappa) monoclonal antibodies (mAbs) against Influenza A/Chicken/Vietnam/8/2004 (H5N1) virus are described. Three of these showed neutralizing activities against H5N1 strains from clades 1, 2 and 3 using a retroviral pseudotype or live virus microneutralization assay. In the pseudotype assay, the IC(90) neutralizing titre range was >1600-51,200, and with the microneutralization was 80> or =10,240. MAb 1C1 showed strong neutralizing activities in both assays. All four mAbs reacted specifically to the immunogen by immunohistochemical staining and to A/Hong Kong/483/1997 (H5N1) and A/Thailand/1(KAN-1)/2004 (H5N1)-infected MDCK cells by immunofluorescence. ELISA titrations of the mAbs showed specificity for H5N1 haemagglutinin (HA) and no cross-reactivity to 15 other Influenza A subtypes. Only mAbs 1C1 and the non-neutralizing 1F7 reacted with HA(1), the cleaved subunit of HA, by Western blot. These results suggest that the mAbs recognize distinct or overlapping epitopes and will be useful reagents for construction of specific rapid point-of-care assays or for therapeutic use.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Oh S,Selleck P,Temperton NJ,Chan PK,Capecchi B,Manavis J,Higgins G,Burrell CJ,Kok Tdoi
10.1016/j.jviromet.2008.12.016subject
Has Abstractpub_date
2009-05-01 00:00:00pages
161-7issue
2eissn
0166-0934issn
1879-0984pii
S0166-0934(08)00464-3journal_volume
157pub_type
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