A method for the purification of rotaviruses and adenoviruses from faeces.

Abstract:

:Rotaviruses and non-cultivable enteric adenoviruses were purified from large volumes of faecal extracts using polyethylene glycol precipitation, centrifugation through 45% w/v sucrose and segregation by density in a cesium chloride density gradient. Yields were high and the preparations were pure enough to use in the production of type-specific antisera and in viral polypeptide analysis. Human rotavirus particles of density 1.38 g/ml were shown to be non-infectious for LLC-MK2 tissue culture cells.

journal_name

J Virol Methods

authors

Beards GM

doi

10.1016/0166-0934(82)90059-3

subject

Has Abstract

pub_date

1982-08-01 00:00:00

pages

343-52

issue

6

eissn

0166-0934

issn

1879-0984

pii

0166-0934(82)90059-3

journal_volume

4

pub_type

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