Abstract：
:Surveillance of wild birds is critical in monitoring for highly pathogenic avian influenza A viruses (AIVs). However, a successful surveillance regime requires proper treatment of samples in the field - rapid placement of samples in -80°C and subsequent maintenance of cold-chain. Given the logistical difficulties of this, many avian taxa and/or geographic locations are not sampled, or, when sampled may result in false negatives due to poor sample treatment in the field. Here, we assessed the utility of RNAlater® as a stabilization agent for AIV sampling. We found no difference in real time PCR performance between virus transport media at optimal conditions and RNAlater® at -80°C, -20°C, 4°C or room temperature up to two weeks, at either low or high virus load. Not only was RNAlater® useful in comparison of spiked samples or those from duck experiments, it was employed successfully in a field study of backyard birds in China. We detected AIV in cloacal and oropharyngeal samples from chickens and a sample with a low Cq was successfully subtyped as H9, although sample storage conditions were suboptimal. Thus, despite limitations in downstream characterization such virus isolation and typing, RNAlater® is a viable option for AIV sampling under logistically challenging circumstances.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Wille M,Yin H,Lundkvist Å,Xu J,Muradrasoli S,Järhult JDdoi
10.1016/j.jviromet.2017.11.004subject
Has Abstractpub_date
2018-02-01 00:00:00pages
32-36eissn
0166-0934issn
1879-0984pii
S0166-0934(17)30494-9journal_volume
252pub_type
杂志文章abstract：:We show that the immuno-gold technique adapted to electron microscopy is a sensitive method for in situ localization of viral proteins in plant cells. Using antisera raised against cauliflower mosaic virus and the viroplasm major protein (VmP) we obtained a selective labelling of the viral particles and of the viropla...
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