当前位置： SCI文献检索 > JOURNAL OF VIROLOGICAL METHODS期刊下所有文献 > Generation and molecular docking analysis of specific single-chain variable fragments selected by phage display against the recombinant nucleocapsid protein of fig mosaic virus.

Generation and molecular docking analysis of specific single-chain variable fragments selected by phage display against the recombinant nucleocapsid protein of fig mosaic virus.

Abstract：

:The mosaic disease caused by fig mosaic virus (FMV) is considered the plague of fig worldwide. A naïve phage display library, raised against the recombinant nucleocapsid protein of FMV (FMV-Np) was screened to obtain specific monoclonal recombinant antibodies in the form of single chain variable fragments (scFvs). After three rounds of biopanning, the bacterially expressed FMV-Np was used as an antigen for selecting specific phages for the production of specific soluble scFvs to be used in immunological assays. The binding specificity of scFvs against FMV-infected fig samples was evaluated by immunoblotting and Plate trapped antigen-ELISA (PTA-ELISA), which revealed efficient of the resultant scFvs to the target antigen. Silico homology-modelling and molecular docking analysis confirmed the scFv and FMV-Np interactions with the anti-FMV-Np scFv through an estimated binding energy of -650 kj mol-1; considered to be generated from the interactions between 13 amino acids residues predicted as putative epitopes in the interface pocket of FMV-Np and scFv antibody. This high affinity was further confirmed in the specificity of ELISA and immunoblotting assays. This is the first report on the application of phage display technology to generate specific recombinant scFvs against FMV that can be applied in development of antibody-mediated protection strategy to control the fig mosaic disease.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Shahmirzaie M,Safarnejad MR,Rakhshandehroo F,Safarpour H,Shirazi FH,Zamanizadeh HR,Elbeaino T

doi

10.1016/j.jviromet.2019.113796

subject

Has Abstract

pub_date

2020-02-01 00:00:00

pages

113796

eissn

0166-0934

issn

1879-0984

pii

S0166-0934(19)30362-3

journal_volume

276

pub_type

杂志文章

相关文献

JOURNAL OF VIROLOGICAL METHODS文献大全
  • Rapid detection of sacbrood virus in honeybee using ultra-rapid real-time polymerase chain reaction.

    abstract：:A real-time reverse transcription-polymerase chain reaction (qRT-PCR) assay was developed for the fast and highly sensitive detection of the sacbrood virus (SBV) genome and applied to honeybee samples. Using plasmid DNA containing a partial SBV genome and diluted serially, as few as 1×10(2)copies/μl (correlation co-ef...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2011.10.014

    authors： Yoo MS,Thi KC,Van Nguyen P,Han SH,Kwon SH,Yoon BS

    更新日期：2012-01-01 00:00:00

  • Development of a combined canine distemper virus specific RT-PCR protocol for the differentiation of infected and vaccinated animals (DIVA) and genetic characterization of the hemagglutinin gene of seven Chinese strains demonstrated in dogs.

    abstract：:A combined reverse-transcription polymerase chain reaction (RT-PCR) method was developed for the detection and differentiation of wild-type and vaccine strains of the canine distemper virus (CDV). A pair of primers (P1/P2) was used to detect both CDV wild-type strains and vaccines. Another pair (P3/P4) was used to det...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2011.11.011

    authors： Yi L,Cheng S,Xu H,Wang J,Cheng Y,Yang S,Luo B

    更新日期：2012-01-01 00:00:00

  • Viral elution and concentration method for detection of influenza A viruses in mud by real-time RT-PCR.

    abstract：:The role of environmental reservoirs in avian influenza virus (AIV) transmission has been investigated during AIV-associated outbreaks. To date, no method has been defined for detection of AIV from mud samples. A procedure using elution and polyethylene glycol (PEG) concentration steps was designed to detect AIV by RT...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2011.10.013

    authors： Deboosere N,Horm SV,Delobel A,Gachet J,Buchy P,Vialette M

    更新日期：2012-01-01 00:00:00

  • A plasmid-based reverse genetics system for mammalian orthoreoviruses driven by a plasmid-encoded T7 RNA polymerase.

    abstract：:Mammalian orthoreoviruses (reoviruses) have served as highly useful models for studies of virus replication and pathogenesis. The development of a plasmid-based reverse genetics system represented a major breakthrough in reovirus research. The current reverse genetics systems for reoviruses rely on the expression of T...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2013.10.023

    authors： Komoto S,Kawagishi T,Kobayashi T,Ikizler M,Iskarpatyoti J,Dermody TS,Taniguchi K

    更新日期：2014-02-01 00:00:00

  • Development of a Plexor real-time PCR assay for the detection of porcine circovirus type 2.

    abstract：:A novel, real-time PCR system for the detection of porcine circovirus type 2 (PCV2) was developed. The system employed Plexor technology and detected 10(8)-10(1) copies per reaction of PCV2 DNA within a recombinant plasmid. The examination of clinical material showed consistent diagnostic sensitivity when samples cont...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2011.11.014

    authors： Vlasakova M,Jackova A,Leskova V,Vilcek S

    更新日期：2012-02-01 00:00:00

  • A comparison of antibody capture radio- and enzyme immunoassays with immunofluorescence for detecting IgM antibody in infants with congenital rubella.

    abstract：:IgM antibody capture radioimmunoassay (MACRIA) and enzyme immunoassay (MACEIA) were compared with immunofluorescence (IF) for detecting specific IgM antibody in 99 sera from 76 infants with confirmed congenital rubella, and 61 sera from a comparative group of 59 infants who had miscellaneous abnormalities but in whom ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(82)90055-6

    authors： Chantler S,Evans CJ,Mortimer PP,Cradock-Watson JE,Ridehalgh MK

    更新日期：1982-08-01 00:00:00

  • Optimal transfection methods and comparison of PK-15 and Dulac cells for rescue of chimeric porcine circovirus type 1-2.

    abstract：:A chimeric porcine circovirus type 1-2 (PCV1-2) infectious DNA clone has low transfection efficiency and exhibits low levels of proliferation. Electroporation and lipofection parameters were optimized for PK-15 and Dulac cells with the purpose of increasing the efficiency for rescuing infectious PCV1-2. Titers of PCV1...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2014.08.005

    authors： Li J,Yu T,Zhou J,Tu W,Gao S,Liu X

    更新日期：2014-11-01 00:00:00

  • Establishment of an indicator cell line for monitoring bovine immunodeficiency virus infection and inhibitor susceptibility.

    abstract：:Indicator cell lines are useful biological tools for monitoring virus infection. In order to monitor infection with bovine immunodeficiency virus (BIV) in vitro, an indicator cell line derived from baby hamster kidney cells which contains integrated copies of an enhanced green fluorescent protein gene driven by the BI...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2009.07.007

    authors： Yao X,Su Y,Liu C,Tan J,Liu L,Geng YQ,Qiao WT

    更新日期：2010-01-01 00:00:00

  • A real-time RT-PCR assay for detection and absolute quantitation of Citrus tristeza virus in different plant tissues.

    abstract：:A real-time RT-PCR assay using SYBR Green was developed for specific and reliable quantitative detection of Citrus tristeza virus (CTV) in infected plants. A general primer set designed from conserved sequences in ORFs 1b and 2 enabled amplification of the genomic RNA (gRNA) while excluding most subgenomic and defecti...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2007.05.011

    authors： Ruiz-Ruiz S,Moreno P,Guerri J,Ambrós S

    更新日期：2007-11-01 00:00:00

  • Recombinant UL30 antigen-based single serum dilution ELISA for detection of duck viral enteritis.

    abstract：:A recombinant UL30 antigen-based single serum dilution enzyme linked immunosorbent assay (ELISA) was developed to measure specific antibody in the sera of ducks against duck enteritis virus (DEV). The partial UL30 gene of DEV was cloned, expressed, purified and tested for its diagnostic use by designing a single serum...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2012.06.027

    authors： Aravind S,Patil BR,Dey S,Mohan CM

    更新日期：2012-11-01 00:00:00

  • Genotyping system of GBV-C/HGV type 1 to type 4 by the polymerase chain reaction using type-specific primers and geographical distribution of viral genotypes.

    abstract：:Based on variation in nucleotide sequence of 5'-untranslated region, GB virus type C (GBV-C) and hepatitis G virus (HGV) can be classified into three major genotypes. In addition to this classification, a novel genotype of GBV-C/HGV was identified and designated type 4. However, genotyping of GBV-C/HGV has been hamper...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(00)00207-x

    authors： Naito H,Abe K

    更新日期：2001-01-01 00:00:00

  • Detection of cytomegalovirus by 24-well plate centrifugation assay using a monoclonal antibody to an early nuclear antigen and by conventional cell culture.

    abstract：:During a 12-month period, two methods for detection of cytomegalovirus (CMV) in 1624 clinical specimens were compared: (1) centrifugal inoculation of MRC-5 cells on coverslips in 24-well plates and staining with a monoclonal antibody to CMV early nuclear antigen after incubation for 40 h (EA assay), and (2) convention...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(87)90082-6

    authors： Woods GL,Young A,Johnson A,Thiele GM

    更新日期：1987-12-01 00:00:00

  • A duplex recombinant viral nucleoprotein microbead immunoassay for simultaneous detection of seroresponses to human respiratory syncytial virus and metapneumovirus infections.

    abstract：:Serologic diagnosis of human respiratory syncytial virus (hRSV) and human metapneumovirus (hMPV) infections has been shown to complement virus detection methods in epidemiologic studies. Enzyme immunoassays (EIAs) using cultured virus lysate antigens are often used to diagnose infection by demonstration of a ≥4-fold r...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2014.05.008

    authors： Zhang Y,Brooks WA,Goswami D,Rahman M,Luby SP,Erdman DD

    更新日期：2014-09-01 00:00:00

  • Optimized approaches for the sequence determination of double-stranded RNA templates.

    abstract：:Double-stranded RNA (dsRNA) is in many cases the only available template for molecular and diagnostic studies of RNA viruses. A novel mycovirus with a five dsRNAs segmented-genome served as a model system for the amplification and cloning of dsRNA segments using several PCR-based methods. Sequences obtained by the cla...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2010.08.013

    authors： Darissa O,Willingmann P,Adam G

    更新日期：2010-11-01 00:00:00

  • Detection of foot-and-mouth disease virus RNA in clinical samples and cell culture isolates by amplification of the capsid coding region.

    abstract：:Foot-and-mouth disease is one of the most economically important virus diseases of livestock. Two important requirements for the control of this disease are rapid laboratory diagnosis and epidemiological investigation. The use of the polymerase chain reaction method (PCR) to amplify specific nucleic acid regions offer...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(93)90176-r

    authors： Höfner MC,Carpenter WC,Donaldson AI

    更新日期：1993-04-01 00:00:00

  • Development of a capsid based competitive inhibition enzyme-linked immunosorbent assay for detection of bovine immunodeficiency virus antibodies in cattle and buffalo serum.

    abstract：:The aim of this study was to develop a more specific and sensitive competitive inhibition ELISA (CI-ELISA) than the currently used indirect ELISA for detection of antibodies to bovine immunodeficiency virus (BIV) in cattle and buffaloes. Murine monoclonal antibodies (MAbs) were generated against a recombinant capsid (...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2007.11.008

    authors： Bhatia S,Sood R,Bhatia AK,Pattnaik B,Pradhan HK

    更新日期：2008-03-01 00:00:00

  • Heparin-binding capacity of the HIV-1 NEF-protein allows one-step purification and biochemical characterization.

    abstract：:Recombinant Nef-protein of HIV-1 Bru derived from Escherichia coli revealed heparin-binding activity. This property was used to purify the Nef-protein by a one-step procedure, yielding about 90% homogenous Nef-protein as evaluated by silver staining. The Nef-protein was soluble without denaturing agents. Native foldin...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(96)02049-6

    authors： Kohleisen B,Gaedigk-Nitschko K,Ohlmann M,Götz E,Ostolaza H,Goni FM,Erfle V

    更新日期：1996-06-01 00:00:00

  • Comparison of a conventional HIV 1/2 line immunoassay with a rapid confirmatory HIV 1/2 assay.

    abstract：:The performance of the rapid confirmatory HIV 1/2 assay Geenius was compared with the conventional HIV 1/2 line immunoblot (INNO-LIA HIV I/II Score). One hundred HIV 1/2 confirmed positive samples from donors and patients and 136 negative screening samples from blood donors were evaluated with both assays. A 20 member...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2014.05.010

    authors： Tinguely C,Schild-Spycher T,Bahador Z,Gowland P,Stolz M,Niederhauser C

    更新日期：2014-09-01 00:00:00

  • Development of SYBR Green based real time PCR assay for detection of monodon baculovirus in Penaeus monodon.

    abstract：:Shrimp farming is one of the most important aquaculture activities. Expansion and intensification of shrimp farming has been accompanied with the outbreak of diseases, which threaten the development and sustainability of the industry. Viral diseases are the major challenges faced by shrimp farming industries. The prev...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2014.05.006

    authors： Ramesh Kumar D,Sanjuktha M,Rajan JJ,Ananda Bharathi R,Santiago TC,Alavandi SV,Poornima M

    更新日期：2014-09-01 00:00:00

  • Addition of exogenous polypeptides on the mammalian reovirus outer capsid using reverse genetics.

    abstract：:Addition of exogenous peptide sequences on viral capsids is a powerful approach to study the process of viral infection or to retarget viruses toward defined cell types. Until recently, it was not possible to manipulate the genome of mammalian reovirus and this was an obstacle to the addition of exogenous sequence tag...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2011.11.021

    authors： Brochu-Lafontaine V,Lemay G

    更新日期：2012-02-01 00:00:00

  • A novel flow cytometry-based tool for determining the efficiency of human cytomegalovirus infection in THP-1 derived macrophages.

    abstract：:Human cytomegalovirus (hCMV) is a ubiquitous pathogen that causes congenital infection and severe infections in immunocompromised patients. Chronic hCMV infection may also play an important role in immunosenescence and adverse health outcomes in older adults. THP-1, a human monocytic cell line and its derived macropha...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2015.04.028

    authors： Li H,Mao G,Carlson J,Leng SX

    更新日期：2015-09-01 00:00:00

  • Serological diagnosis of influenza A and B infections by enzyme immunoassay. Comparison with the complement fixation test.

    abstract：:Paired sera from 784 patients with symptoms of acute respiratory disease were examined for antibodies against influenza A, B and parainfluenza (1 and 3) viruses by complement fixation (CF) and enzyme immunoassay (EIA). The internal variation of the EIA test results was low and an increase of 0.250 in absorbance values...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(84)90078-8

    authors： Julkunen I,Kleemola M,Hovi T

    更新日期：1984-08-01 00:00:00

  • Detection of baboon cytomegalovirus (BaCMV) by PCR using primers directed against the glycoprotein B gene.

    abstract：:We have cloned and sequenced the glycoprotein B genes from five strains of BaCMV, isolated from three subspecies of cynocephalus baboons (olive, yellow and chacma). Primers were designed using conserved DNA regions of the gB gene to allow DNA amplification from all strains of BaCMV. These regions differ sufficiently f...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2005.01.010

    authors： Ross TG,Rogers RP,Elfrink N,Bray N,Blewett EL

    更新日期：2005-05-01 00:00:00

  • Inducible model to study negative strand RNA synthesis and assembly of hepatitis C virus from a full-length cDNA clone.

    abstract：:An inducible in vitro cell culture system was developed to assay HCV replication by direct biochemical means. A transcription plasmid containing a T7 promoter at the 5' end, full-length cDNA of the HCV genome, a ribozyme sequence from the antigenomic strand of hepatitis delta virus and a T7 terminator was prepared. To...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(01)00278-6

    authors： Myung J,Khalap N,Kalkeri G,Garry R,Dash S

    更新日期：2001-05-01 00:00:00

  • A high-throughput assay for HIV-1 integrase 3'-processing activity using time-resolved fluorescence.

    abstract：:HIV-1 integrase (HIV-1 IN), a well-validated antiviral drug target, catalyzes multistep reactions to incorporate viral DNA into the genome of the host cell; these include both a 3'-processing (3'P) reaction and a strand transfer reaction. These enzymatic activities can be measured in vitro with short DNA oligonucleoti...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2012.05.003

    authors： Han YS,Quashie P,Mesplede T,Xu H,Mekhssian K,Fenwick C,Wainberg MA

    更新日期：2012-09-01 00:00:00

  • Evaluation of SYBR Green real time PCR for detecting SARS-CoV-2 from clinical samples.

    abstract：:The pandemic caused by SARS-CoV-2 has triggered an extraordinary collapse of healthcare systems and hundred thousand of deaths worldwide. Following the declaration of the outbreak as a Public Health Emergency of International Concern by the World Health Organization (WHO) on January 30th, 2020, it has become imperativ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2020.114035

    authors： Pereira-Gómez M,Fajardo Á,Echeverría N,López-Tort F,Perbolianachis P,Costábile A,Aldunate F,Moreno P,Moratorio G

    更新日期：2020-12-04 00:00:00

  • Transmission of viral RNA and DNA to maize kernels by vascular puncture inoculation.

    abstract：:Vascular puncture inoculation (VPI) is an effective technique for transmission of maize viruses without using arthropods or other biological vectors. It involves using a jeweler's engraving tool to push minuten pins through a droplet of virus inoculum toward the major vascular bundle in the scutellum of germinating ke...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(01)00369-x

    authors： Redinbaugh MG,Louie R,Ngwira P,Edema R,Gordon DT,Bisaro DM

    更新日期：2001-11-01 00:00:00

  • A multiplex real-time PCR assay for the detection and differentiation of the newly emerged porcine circovirus type 3 and continuously evolving type 2 strains in the United States.

    abstract：:A multiplex quantitative real-time polymerase chain reaction (mqPCR) assay was developed and validated for the detection and differentiation of porcine circovirus type 3 (PCV3) and type 2 (PCV2) strains. The assay coverage was 97.9% (184/188) for PCV3 and 99.1% (1889/1907) for PCV2 sequences that were available from t...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2019.03.011

    authors： Wang Y,Feng Y,Zheng W,Noll L,Porter E,Potter M,Cino G,Peddireddi L,Liu X,Anderson G,Bai J

    更新日期：2019-07-01 00:00:00

  • Detection of circulating platelet-monocyte complexes in persons infected with human immunodeficiency virus type-1.

    abstract：:Activated platelets form transient aggregates with monocytes in circulation and have a half-life of approximately 30-60 min. These complexes are increased in various inflammatory conditions and are an early marker of myocardial infarction. HIV-1 infection is associated with chronic inflammation, and increased CD16⁺ in...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2012.02.005

    authors： Singh MV,Davidson DC,Kiebala M,Maggirwar SB

    更新日期：2012-05-01 00:00:00

  • Separation of near full-length hepatitis C virus quasispecies variants from a complex population.

    abstract：:A long RT-PCR (LRP) protocol was developed recently for robust amplification of a near full-length HCV genomic sequence from clinical samples, followed by efficient cloning [Fan, X., Xu, Y., Di Biceglie, A.M., 2006. Efficient amplification and cloning of near full-length hepatitis C virus genome from clinical samples....

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2006.12.002

    authors： Zhou D,Fan X,Tan D,Xu Y,Tavis JE,Di Bisceglie AM

    更新日期：2007-05-01 00:00:00