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Serological diagnosis of influenza A and B infections by enzyme immunoassay. Comparison with the complement fixation test.

Abstract：

:Paired sera from 784 patients with symptoms of acute respiratory disease were examined for antibodies against influenza A, B and parainfluenza (1 and 3) viruses by complement fixation (CF) and enzyme immunoassay (EIA). The internal variation of the EIA test results was low and an increase of 0.250 in absorbance values which corresponded to a two-fold increase in end-point titres was considered a diagnostic antibody rise. EIA detected significantly more diagnostic rises than the CF test in the case of influenza A (222 vs. 162, P less than 0.001) and parainfluenza virus antibodies (29 vs. 16, P less than 0.01). More diagnostic rises in influenza B antibodies were also observed by EIA compared to the CF test (104 vs. 99, not significant). There were only two patients who showed a diagnostic rise in CF antibodies (both influenza B) but not in EIA. Most often patients with a diagnostic antibody rise only by the EIA method had a two-fold rise in the respective CF antibodies (68% of cases). EIA was found to be a sensitive and reliable method for the serological diagnosis of influenza A, B and parainfluenza infections.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Julkunen I,Kleemola M,Hovi T

doi

10.1016/0166-0934(84)90078-8

subject

Has Abstract

pub_date

1984-08-01 00:00:00

pages

7-14

issue

1

eissn

0166-0934

issn

1879-0984

pii

0166-0934(84)90078-8

journal_volume

9

pub_type

杂志文章

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