Abstract：
:Hepatitis A virus (HAV) is an important cause of foodborne disease worldwide. The detection of this virus in naturally contaminated food products is complicated by the absence of a reliable culture method, low levels of contamination, and the presence of matrix-associated compounds which inhibit molecular detection. In this study, we report a novel method to concentrate HAV from foods prior to the application of reverse transcription-PCR (RT-PCR) for detection. Specifically, we used cationically charged magnetic particles with an automated capture system (Pathatrix) to concentrate the virus from 25 g samples of artificially contaminated lettuce, strawberries, green onions, deli-turkey, oysters, and cake with frosting. Detection limits varied according to the product but in most cases, the virus could be consistently detected at input levels corresponding to 10(2)PFU/25 g food sample. For some products, detection was possible at levels as low as 10(-1)PFU/25 g. The assay was applied by a second independent laboratory and was also used to confirm viral contamination of produce items associated with a recent HAV outbreak. Parallel infectivity assays demonstrated that the cationically charged particles bound approximately 50% of the input virus. This is the first application of the automated magnetic capture technology to the concentration of viruses from foods, and it offers promise for facilitating the rapid detection of HAV from naturally contaminated products.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Papafragkou E,Plante M,Mattison K,Bidawid S,Karthikeyan K,Farber JM,Jaykus LAdoi
10.1016/j.jviromet.2007.08.024subject
Has Abstractpub_date
2008-01-01 00:00:00pages
177-87issue
1eissn
0166-0934issn
1879-0984pii
S0166-0934(07)00353-9journal_volume
147pub_type
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