Abstract：
:Arenaviruses merit significant attention both as causative agents of endemic hemorrhagic fevers and as model systems to study the immune response to acute and persistent viral infections. Development of highly sensitive quantitative screening methods to detect arenavirus is critical for early diagnosis of patients, to screen the rodent population in endemic areas, and as a research tool to confirm effective tissue clearance during the development of anti-viral strategies. This study describes a novel sensitive and reproducible method to quantify prototypic new world arenavirus Tacaribe RNA in cell cultures and tissues using a real-time TaqMan PCR-based detection system. The method has a sensitivity of 100 RNA copies per 200ng of total RNA, making it 2 logs more sensitive than the currently utilized TCID(50) method, and a linear range from 10(2) to 10(9) copies/reaction. The qRT-PCR method is high-throughput and screening can be achieved in <2h allowing for diagnosis of infected patients before the onset of symptoms. This new method is a powerful tool to screen populations for infection and monitor the clearance achieved by available therapies, and serves as a model diagnostic tool for other arenaviruses.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Grajkowska LT,Pedras-Vasconcelos JA,Sauder C,Verthelyi D,Puig Mdoi
10.1016/j.jviromet.2009.04.001subject
Has Abstractpub_date
2009-08-01 00:00:00pages
239-43issue
2eissn
0166-0934issn
1879-0984pii
S0166-0934(09)00165-7journal_volume
159pub_type
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