Abstract：
:Rift Valley fever virus (RVFV) is an arthropod-borne pathogen that often results in severe morbidity and mortality in both humans and livestock. As its geographic range continues to expand, it presents a real threat to naïve populations around the world by accidental introduction (e.g., the result of increased travel) or intentional release (e.g., a bioterror event). While there is a clear need for a safe and efficacious vaccine against this emerging and re-emerging pathogen, no FDA-approved vaccine is currently available. This need was addressed by the establishment of novel mammalian and insect suspension cell line systems for the efficient production of RVF virus-like particle (VLP)-based vaccine candidates. A direct comparison of the production of RVF VLPs in these systems was performed. Optimization and characterization resulted in a production platform suitable for scale-up. Furthermore, RVF VLP-based vaccines were tested in a lethal challenge model and showed full protection, demonstrating that RVF VLPs present promising RVFV vaccine candidates.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Mandell RB,Koukuntla R,Mogler LJ,Carzoli AK,Holbrook MR,Martin BK,Vahanian N,Link CJ,Flick Rdoi
10.1016/j.jviromet.2010.07.015subject
Has Abstractpub_date
2010-11-01 00:00:00pages
259-68issue
2eissn
0166-0934issn
1879-0984pii
S0166-0934(10)00257-0journal_volume
169pub_type
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