Abstract：
:A chimeric porcine circovirus type 1-2 (PCV1-2) infectious DNA clone has low transfection efficiency and exhibits low levels of proliferation. Electroporation and lipofection parameters were optimized for PK-15 and Dulac cells with the purpose of increasing the efficiency for rescuing infectious PCV1-2. Titers of PCV1-2 in Dulac cells were 100-fold higher than those in PK-15 cells following transfection. The electroporation efficiency into Dulac cells was high when three 400 μs pulses at 250 V with 6 μg of plasmid DNA was used, lipofection efficiency was high when the ratio of DNA to transfection reagent was 1:3. The proportion of infected cells was 55.6% compared with 44.2%, for the electroporation and lipofection techniques respectively. Virus titers were higher in Dulac cells, from 10(4.44) to 10(5.32)TCID50/mL compared with 10(1.90)-10(3.38)TCID(50)/mL for PK-15 cells. Dulac cells were more permissive to PCV1-2 than PK-15 cells regardless of the transfection technique.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Li J,Yu T,Zhou J,Tu W,Gao S,Liu Xdoi
10.1016/j.jviromet.2014.08.005subject
Has Abstractpub_date
2014-11-01 00:00:00pages
90-5eissn
0166-0934issn
1879-0984pii
S0166-0934(14)00312-7journal_volume
208pub_type
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