Abstract：
:The aim of this study was to evaluate the applicability of virus concentration methods to detect human norovirus (HuNoV) in water. One conventional virus concentration method using an electropositive filter (1MDS-method) and two methods developed by our research group using an electronegative filter (Mg-method and Al-method) were subjected to recovery tests of the HuNoV strain GII.4, which was obtained from a diarrhea patient, and poliovirus (PV) type 1 inoculated into 5 kinds of water samples. The mean recovery yields of HuNoV by the Mg-method, determined by real-time PCR, were 186%, 80%, 167%, 15%, and 39% for MilliQ water, tap water, bottled water, river water, and pond water, respectively (n=2 each), which were generally comparable to those of PV. A similar trend was observed for the Al-method (n=8 in total), suggesting that both Mg- and Al-methods can be appropriate for concentrating HuNoVs from water samples. Unlike these two methods, no clear correlation was found between the recovery of HuNoV and PV by the 1MDS-method (n=6 in total). This study is significant because it provides observations on the use of virus concentration methods for the detection of uncultivable HuNoV in water samples.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Haramoto E,Katayama H,Utagawa E,Ohgaki Sdoi
10.1016/j.jviromet.2009.05.002subject
Has Abstractpub_date
2009-09-01 00:00:00pages
206-9issue
1-2eissn
0166-0934issn
1879-0984pii
S0166-0934(09)00243-2journal_volume
160pub_type
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