Abstract：
:A method for detecting the emergence of potential pandemic-causing influenza strains has been developed. The system first uses real-time RT-PCR to detect H5, the highly pathogenic avian influenza subtype most likely to cause a pandemic. Pyrosequencing is then employed to scan for codon changes encoding amino acids known to define human influenza versus avian influenza signatures. The pyrosequencing assays were developed to screen at the nucleotide level for 52 amino acid changes defined as avian- or human-specific. A library has been built to screen the sequence data generated and properly identify the strain in question as a potential threat. This method can be used to screen samples for influenza and to determine if the detected virus contains mutations that may make the virus more infective or virulent to humans, potentially thwarting a pandemic outbreak.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Waybright N,Petrangelo E,Lowary P,Bogan J,Mulholland Ndoi
10.1016/j.jviromet.2008.09.013subject
Has Abstractpub_date
2008-12-01 00:00:00pages
200-5issue
1-2eissn
0166-0934issn
1879-0984pii
S0166-0934(08)00329-7journal_volume
154pub_type
杂志文章abstract：:Poxviruses are emerging pathogens in cetaceans, temporarily named 'Cetaceanpoxvirus' (CePV, family Poxviridae), classified into two main lineages: CePV-1 in odontocetes and CePV-2 in mysticetes. Only a few studies performed the molecular detection of CePVs, based on DNA-polymerase gene and/or DNA-topoisomerase I gene ...
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/s0166-0934(01)00278-6
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abstract：:In common with latent viruses such as herpesviruses, parvovirus B19, HBV and GBV-C are contained successfully by the immune response and persist in the host. When immune control breaks down, reactivation of both latent and persistent viruses occurs. Two multiplex assays were developed (B19, HBV, HHV-8), (EBV, CMV, VZV...
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pub_type： 杂志文章
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journal_title：Journal of virological methods
pub_type： 杂志文章
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journal_title：Journal of virological methods
pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2002-03-01 00:00:00
abstract：:It remains unclear whether hepatitis B virus (HBV) replicates in extrahepatic tissues, and particularly in peripheral blood mononuclear cells (PBMCs), which may serve as a reservoir for the maintenance of infection. A real-time PCR assay for the quantitation of total and covalently closed circular (ccc) HBV DNA in ser...
journal_title：Journal of virological methods
pub_type： 杂志文章
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更新日期：2006-12-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2012.02.008
更新日期：2012-05-01 00:00:00
abstract：:Highly pathogenic avian influenza virus H5N1 is a continuous threat to public health and poultry industry. The recurrence of the H5N1 led us to develop a robust, specific, and rapid detection method for the virus. In this study, an impedance aptasensor was developed for the virus detection using specific H5N1 aptamer ...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2016.07.018
更新日期：2016-10-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2010.09.023
更新日期：2011-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.jviromet.2011.02.002
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pub_type： 杂志文章
doi：10.1016/0166-0934(87)90072-3
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journal_title：Journal of virological methods
pub_type： 临床试验,杂志文章
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pub_type： 杂志文章
doi：10.1016/0166-0934(85)90038-2
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journal_title：Journal of virological methods
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doi：10.1016/j.jviromet.2009.11.003
更新日期：2010-02-01 00:00:00
abstract：:To formulate inactivated influenza vaccines, the concentration of hemagglutinin (HA) must be accurately determined. The standard test currently used to measure HA in influenza vaccines is the Single Radial Immunodiffusion (SRID) assay. We developed a very rapid, simple and sensitive alternative quantitative HA assay, ...
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pub_type： 杂志文章
doi：10.1016/j.jviromet.2017.10.011
更新日期：2018-01-01 00:00:00
abstract：:Mammalian orthoreoviruses (reoviruses) have served as highly useful models for studies of virus replication and pathogenesis. The development of a plasmid-based reverse genetics system represented a major breakthrough in reovirus research. The current reverse genetics systems for reoviruses rely on the expression of T...
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journal_title：Journal of virological methods
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Journal of virological methods
pub_type： 杂志文章
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journal_title：Journal of virological methods
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