Abstract：
:Human coronavirus NL63 was identified in 2004 in the Netherlands. Due to the high prevalence and world-wide distribution of this pathogen, it is essential to develop a sensitive and specific detection assay suitable for use in a routine diagnostic laboratory. Techniques based on PCR or real-time PCR are laborious and expensive. Detailed analysis of the HCoV-NL63 genome permitted the identification of a conserved nucleic acid sequential motif, which was sufficient for the design of a loop-mediated isothermal amplification (LAMP) assay. Evaluation of the method showed that the test is specific to HCoV-NL63 and that it does not cross-react with other respiratory viruses. The detection limit was found to be 1 copy of RNA template per reaction in cell culture supernatants and clinical specimens.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Pyrc K,Milewska A,Potempa Jdoi
10.1016/j.jviromet.2011.04.024subject
Has Abstractpub_date
2011-07-01 00:00:00pages
133-6issue
1eissn
0166-0934issn
1879-0984pii
S0166-0934(11)00162-5journal_volume
175pub_type
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