Abstract：
:Two mosquito-borne flaviviruses, Murray Valley encephalitis (MVE) and Kunjin (KUN), are the aetiological agents of Australian encephalitis. MVE causes a severe and potentially fatal form of the disease while KUN is responsible for only a few relatively mild cases. Therefore it is important that serological tests used in flavivirus surveillance differentiate between infections with these two viruses. However, this has been hampered in the past by the close antigenic relationships between flaviviruses in traditional serological assays. An epitope blocking ELISA using MVE-specific and KUN-specific monoclonal antibodies (mAb) reacting to the non-structural protein NS1 of these viruses and a flavivirus group-specific mAb reacting to the envelope (E) protein was assessed for testing sentinel animals for seroconversion to specific flavivirus infections. Using these assays we were able to detect serum antibodies to a variety of flavivirus in laboratory infected rabbits, and naturally infected chickens and in the case of primary infections, differentiate those caused by KUN or MVE. These assays are now used routinely in our laboratory for testing chicken sera from sentinel flocks in the Kimberley and Pilbara regions of north Western Australia.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Hall RA,Broom AK,Hartnett AC,Howard MJ,Mackenzie JSdoi
10.1016/0166-0934(94)00105-psubject
Has Abstractpub_date
1995-02-01 00:00:00pages
201-10issue
2-3eissn
0166-0934issn
1879-0984pii
0166-0934(94)00105-Pjournal_volume
51pub_type
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