当前位置： SCI文献检索 > JOURNAL OF VIROLOGICAL METHODS期刊下所有文献 > Evaluation of the cobas® HCV test for quantifying HCV RNA in dried plasma spots collected using the cobas® Plasma Separation Card.

Evaluation of the cobas® HCV test for quantifying HCV RNA in dried plasma spots collected using the cobas® Plasma Separation Card.

Abstract：

OBJECTIVE:This study evaluated the performance of the cobas® hepatitis C virus (HCV) Test for use on the cobas® 6800/8800 Systems for the detection and quantification of HCV RNA collected using the cobas® Plasma Separation Card (PSC) compared with plasma samples. METHODS:Whole EDTA-venous blood was collected from 50 HCV-positive donors and 140 μL from each donor was spotted onto a PSC and stored either frozen or at ambient temperature. These were compared with matched EDTA-plasma samples. The limit of detection (LoD) of the assay for PSC samples was determined using serial dilutions of the Roche HCV secondary calibration standard. The stability of the PSC samples across a range of timepoints was also assessed. RESULTS:The mean titer difference between ambient and -10 °C storage of PSC samples was 0.04 log10 IU/mL (95% CI: 0.00, 0.07). The mean titer difference between frozen PSC samples and EDTA plasma samples was -1.59 log10 IU/mL (95% CI: -1.66, -1.53) and between ambient PSC samples and EDTA samples was -1.64 log10 IU/mL (95% CI: -1.70, -1.57). Correlation between PSC samples and EDTA plasma was linear in both cases (frozen: slope = 1.039, intercept=-1.839, R2 = 0.89; ambient: slope = 1.012, intercept=-1.712, R2 = 0.88). The LoD of the cobas® HCV Test using the PSC was 866 IU/mL (95% CI: 698, 1153 IU/mL) and 408 IU/mL (95% CI: 336, 544 IU/mL) using an optimized Assay Specific Analysis Package. CONCLUSIONS:PSC samples correlate well with plasma viral load and demonstrate a LoD below 1000 IU/mL and good stability at ambient temperature for 28 days. A correction factor would allow quantification of HCV viral RNA load from samples collected using a PSC.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Marins EG,Krey N,Becker A,Melzer S,Hoppler M

doi

10.1016/j.jviromet.2020.113820

subject

Has Abstract

pub_date

2020-04-01 00:00:00

pages

113820

eissn

0166-0934

issn

1879-0984

pii

S0166-0934(19)30501-4

journal_volume

278

pub_type

杂志文章

相关文献

JOURNAL OF VIROLOGICAL METHODS文献大全
  • Validation of a foot-and-mouth disease antibody screening solid-phase competition ELISA (SPCE).

    abstract：:This paper describes the validation of a solid-phase competition enzyme-linked immunosorbent assay (SPCE) for the serological detection of antibody to serotype O foot-and-mouth disease (FMD) in sheep, cattle and pigs. The specificity of the SPCE was calculated from the results of testing known negative sera from sheep...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2003.09.016

    authors： Paiba GA,Anderson J,Paton DJ,Soldan AW,Alexandersen S,Corteyn M,Wilsden G,Hamblin P,MacKay DK,Donaldson AI

    更新日期：2004-02-01 00:00:00

  • A novel and inexpensive application of RNAi technology to protect shrimp from viral disease.

    abstract：:Large-scale production of long dsRNA is needed if antiviral applications of RNAi are to succeed in shrimp farm operations. A novel hairpin-RNA expression vector was developed based on the RNA-dependent RNA polymerase (RdRp) gene of yellow head virus (YHV), the cause of a lethal shrimp disease. Using transformed RNase-...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2009.08.010

    authors： Saksmerprome V,Charoonnart P,Gangnonngiw W,Withyachumnarnkul B

    更新日期：2009-12-01 00:00:00

  • The impact of PCR-generated recombination on diversity estimation of mixed viral populations by deep sequencing.

    abstract：:Ultra-deep pyrosequencing (UDPS) of targeted amplicons allows to determine a large number of individual sequence reads from a single PCR product, and this approach is thus extremely valuable for analysis of mixed viral populations. A mixture of genetically distinct DNA templates, however, may lead to the generation of...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2010.07.040

    authors： Görzer I,Guelly C,Trajanoski S,Puchhammer-Stöckl E

    更新日期：2010-10-01 00:00:00

  • A method for full genome sequencing of all four serotypes of the dengue virus.

    abstract：:The availability of whole genome sequencing has contributed to many aspects of dengue research, and its use in dengue virus (DENV) surveillance for early epidemic warning has been proposed. Methods to sequence the genomes of individual dengue serotypes have been described previously, but no single method is known to b...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2010.06.013

    authors： Christenbury JG,Aw PP,Ong SH,Schreiber MJ,Chow A,Gubler DJ,Vasudevan SG,Ooi EE,Hibberd ML

    更新日期：2010-10-01 00:00:00

  • Simultaneous detection of eight swine reproductive and respiratory pathogens using a novel GeXP analyser-based multiplex PCR assay.

    abstract：:A new high-throughput GenomeLab Gene Expression Profiler (GeXP) analyser-based multiplex PCR assay was developed for the detection of eight reproductive and respiratory pathogens in swine. The reproductive and respiratory pathogens include North American porcine reproductive and respiratory syndrome virus (PRRSV-NA), ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2015.08.001

    authors： Zhang M,Xie Z,Xie L,Deng X,Xie Z,Luo S,Liu J,Pang Y,Khan MI

    更新日期：2015-11-01 00:00:00

  • A rapid assay for Hendra virus IgG antibody detection and its titre estimation using magnetic nanoparticles and phycoerythrin.

    abstract：:Detection of Hendra viral IgG antibody in animal sera is useful for surveillance following a virus outbreak. The commonly used enzyme-linked immunosorbent assay and fluorescence-based Luminex assay typically consist of three steps and take at least several hours to complete. We have simplified the procedure to two ste...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2015.05.008

    authors： Gao Y,Pallister J,Lapierre F,Crameri G,Wang LF,Zhu Y

    更新日期：2015-09-15 00:00:00

  • An ELISA-inhibition assay for antibody to human immunodeficiency virus core antigen (p24).

    abstract：:An ELISA-inhibition assay based on commercially available HIV-1 p24 antigen tests was developed for detecting p24 antibodies. The test is specific and simple. p24 antibody was detectable in all p24 antigen negative Western blot positive sera, but was detectable infrequently in antigen positive sera. Sera from patients...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(90)90048-k

    authors： Povolotsky J,Gold JW,Darminin P,Chein N,Baron P,Armstrong D

    更新日期：1990-10-01 00:00:00

  • Ovine progressive pneumonia virus capsid is B-cell immunodominant using Western blot analysis: a comparison of sensitivity between Western blot analysis and immunoprecipitation.

    abstract：:A Western blot assay was developed and analyzed against the comparable standard, immunoprecipitation of (35)[S]-methionine/cysteine-labeled ovine progressive pneumonia virus (OPPV) proteins, for its ability to detect anti-OPPV antibodies using endpoint titers. Western blot analysis is 12-fold more sensitive in detecti...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2006.06.025

    authors： Myers-Evert DK,Herrmann-Hoesing LM

    更新日期：2006-11-01 00:00:00

  • Use of internal standard RNA molecules for the RT-PCR amplification of the faeces-borne RNA viruses.

    abstract：:The diagnostic system based on reverse transcription (RT)-PCR has been used widely for the detection of viral genomes of faecal-borne RNA viruses. However, faecal specimens often produce both false positive and false negative results. Therefore, there is a need for a diagnosis procedure that can control for 'false-res...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(02)00016-2

    authors： Kim K,Park J,Chung Y,Cheon D,Lee IB,Lee S,Yoon J,Cho H,Song C,Lee KH

    更新日期：2002-07-01 00:00:00

  • Evaluation of a prototype HCV NS5b assay for typing strains of hepatitis C virus isolated from Tunisian haemodialysis patients.

    abstract：:Hepatitis C virus (HCV) strains isolated from 68 haemodialysis Tunisian patients exhibiting chronic infection were genotyped targeting the NS5b region of the HCV genome using a prototype assay developed by Bayer HealthCare-Diagnostics (TRUGENE NS5b HCV). The overall results were compared to those obtained with another...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2004.03.002

    authors： Othman SB,Trabelsi A,Monnet A,Bouzgarrou N,Grattard F,Beyou A,Bourlet T,Pozzetto B

    更新日期：2004-08-01 00:00:00

  • Relationship between plant virus concentration and infectivity: a 'growth curve' model.

    abstract：:A logistic ('growth curve') model is formulated and applied to the relationship between numbers of infections (local lesions) produced by a virus on inoculated plants and the concentration of the virus in the inoculum. This model has the advantages of being simple, data-based and therefore not founded on limiting post...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(87)90084-x

    authors： Gokhale DV,Bald JG

    更新日期：1987-12-01 00:00:00

  • Detection of human papillomavirus DNA and mRNA using synthetic, type-specific oligonucleotide probes.

    abstract：:Type-specific 30'mer-36'mer oligonucleotide probes complementary to mRNA transcribed from the E6 and E7 open reading frames of HPV 6b/11, 16, 18 and 33 were designed using the published nucleotide sequences. As oligonucleotides are easily and relatively cheaply synthesized in large amounts and are free of vector DNA, ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(93)90018-m

    authors： Faulkner-Jones BE,Tabrizi SN,Borg AJ,Roche PJ,Haralambidis J,Coghlan JP,Garland SM

    更新日期：1993-03-01 00:00:00

  • Importance of method in the determination of syncytium-inducing phenotype of human immunodeficiency virus type 1 clinical isolates.

    abstract：:The in vitro syncytium induction capacity of human immunodeficiency virus type 1 (HIV1) isolates is an important marker in the progression of the disease. Two methods have been widely used to determine the biological phenotype of HIV1. These two methods, the direct MT-2 assay and the supernatant assay, were compared f...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(96)02152-0

    authors： Liesnard C,Delforge ML,Tchetcheroff M,De Maertelaer V,Farber CM,Van Vooren JP

    更新日期：1997-03-01 00:00:00

  • Detection and quantitation of group A rotaviruses by competitive and real-time reverse transcription-polymerase chain reaction.

    abstract：:A competitive reverse transcription-polymerase chain reaction (RT-PCR) was developed to detect and to quantitate the RNA of group A rotaviruses. In the assay, a 433 bp fragment is amplified by a one-tube RT-PCR protocol using primers with binding sites located in a highly conserved region of segment 6 of the rotavirus...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(02)00118-0

    authors： Schwarz BA,Bange R,Vahlenkamp TW,Johne R,Müller H

    更新日期：2002-09-01 00:00:00

  • HSV-1 amplicon peptide display vector.

    abstract：:There are significant uses for expressing foreign peptide epitopes in viral surface attachment proteins in terms of investigating viral targeting, biology, and immunology. HSV-1 attachment, followed by fusion and entry, is mediated in large part by the binding of viral surface glycoproteins to cell surface receptors, ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(02)00193-3

    authors： Spear MA,Schuback D,Miyata K,Grandi P,Sun F,Yoo L,Nguyen A,Brandt CR,Breakefield XO

    更新日期：2003-01-01 00:00:00

  • Low abundance drug resistance variants in transmitted HIV drug resistance surveillance specimens identified using tagged pooled pyrosequencing.

    abstract：:HIV drug resistance (DR) testing using Sanger sequencing (SS) is limited by the inability of the method to identify low abundance drug resistance variants. The application of tagged pooled pyrosequencing (TPP) for HIV DR surveillance is described and the results compared with SS. HIV(+) serum specimens were genotyped ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2012.10.018

    authors： Ji H,Liang B,Li Y,Van Domselaar G,Graham M,Tyler S,Merks H,Sandstrom P,Brooks J

    更新日期：2013-02-01 00:00:00

  • Chemical inactivation of hepatitis B virus: the effect of disinfectants on virus-associated DNA polymerase activity, morphology and infectivity.

    abstract：:The inactivation of hepatitis B virus (HBV) using two commercially available disinfectants was analysed. Indirect evidence of virus inactivation was obtained by examining the decrease in HBV-associated DNA polymerase and HBcAg activities after treatment with increasing concentrations of disinfectant. Inactivation was ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(83)90003-4

    authors： Howard CR,Dixon J,Young P,van Eerd P,Schellekens H

    更新日期：1983-09-01 00:00:00

  • Heparin-binding capacity of the HIV-1 NEF-protein allows one-step purification and biochemical characterization.

    abstract：:Recombinant Nef-protein of HIV-1 Bru derived from Escherichia coli revealed heparin-binding activity. This property was used to purify the Nef-protein by a one-step procedure, yielding about 90% homogenous Nef-protein as evaluated by silver staining. The Nef-protein was soluble without denaturing agents. Native foldin...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(96)02049-6

    authors： Kohleisen B,Gaedigk-Nitschko K,Ohlmann M,Götz E,Ostolaza H,Goni FM,Erfle V

    更新日期：1996-06-01 00:00:00

  • Enzyme-linked fluorescence immunoassays using beta-galactosidase and antibodies covalently bound to polystyrene plates.

    abstract：:A solid-phase enzyme-linked immunoassay using a fluorogenic substrate (4-methylumbelliferyl-beta-D-galactopyranoside) was developed. Antibodies were covalently linked to glutaraldehyde-activated 96-well aminopolystyrene plates. Antigens from test samples were adsorbed to the solid phase and detected using antibodies c...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(81)90050-1

    authors： Neurath AR,Strick N

    更新日期：1981-10-01 00:00:00

  • Development and evaluation of a reverse transcription loop-mediated isothermal amplification assay for H3N8 equine influenza virus.

    abstract：:Reverse transcription loop-mediated isothermal amplification (RT-LAMP) was applied to the detection of equine influenza virus (EIV). Because equine influenza is caused currently by EIV of the H3H8 subtype, the RT-LAMP primer set was designed to target the hemagglutinin gene of this subtype. The detection limit of the ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2011.07.015

    authors： Nemoto M,Yamanaka T,Bannai H,Tsujimura K,Kondo T,Matsumura T

    更新日期：2011-12-01 00:00:00

  • A method for the detection and confirmation of antibodies to hepatitis C virus in dried blood spots.

    abstract：:This study describes the development and evaluation of a cost effective test rationale for the detection of anti-HCV in dried blood spots. Samples were screened using an 'in house' IgG ELISA that incorporated the recombinant proteins c22-3, c200 and NS5. Confirmation of specific antibody to HCV was by a modification o...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(97)00127-4

    authors： Parker SP,Cubitt WD,Ades AE

    更新日期：1997-11-01 00:00:00

  • Development and evaluation of a new epitope-blocking ELISA for universal detection of antibodies to West Nile virus.

    abstract：:West Nile virus (WNV) is an emerging zoonotic pathogen with a wide range of hosts, including birds, horses and humans. The development and evaluation of the performance of a new enzyme-linked immunosorbent assay (ELISA) are described for rapid detection of WNV-specific antibodies in samples originating from an extensi...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2011.03.015

    authors： Sotelo E,Llorente F,Rebollo B,Camuñas A,Venteo A,Gallardo C,Lubisi A,Rodríguez MJ,Sanz AJ,Figuerola J,Jiménez-Clavero MÁ

    更新日期：2011-06-01 00:00:00

  • Relative sensitivity and specificity of agar gel immunodiffusion, enzyme immunosorbent assay, and immunoblotting for detection of anti-bovine leukemia virus antibodies in cattle.

    abstract：:Three serologic methods for the detection of antibodies to bovine leukemia virus (BLV) were compared using the sera of 140 dairy cows. A widely used commercial agarose immunodiffusion screening assay and a commercial antibody capture enzyme immunosorbent assay were compared for sensitivity and specificity with immunob...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(02)00040-x

    authors： Choi KY,Liu RB,Buehring GC

    更新日期：2002-06-01 00:00:00

  • Analytical evaluation of the microarray-based FluChip-8G Influenza A+B Assay.

    abstract：BACKGROUND:Influenza causes a significant annual disease burden, with characterization of the infecting virus important in clinical and public health settings. Rapid immunoassays are fast but insensitive, whereas real-time RT-PCR is sensitive but susceptible to genetic mutations and often requires multiple serial assay...

    journal_title：Journal of virological methods

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.jviromet.2019.113686

    authors： Taylor AW,Dawson ED,Blair RH,Johnson JE Jr,Slinskey AH,Smolak AW,Toth E,Liikanen K,Stoughton RS,Smith C,Talbot S,Rowlen KL

    更新日期：2019-11-01 00:00:00

  • Simple serological diagnosis of arboviruses: a simplified diluent system for Japanese encephalitis virus hemagglutination-inhibition tests using formalinized chick erythrocytes.

    abstract：:Formalinized chick erythrocytes agglutinated with Japanese encephalitis virus antigens over a wider range of pH (6.0-7.2) than fresh erythrocytes (pH 6.4-6.8). The virus antigens were unstable below pH 7.0 and therefore the use of the formalinized erythrocytes made it possible for a one-diluent system of the hemagglut...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(80)90026-9

    authors： Ito H,Iwasa S

    更新日期：1980-01-01 00:00:00

  • A rapid and sensitive micro scale assay for quantitative detection of cell protective effects: application for the isolation of a monoclonal antibody against HeLa cell proteins involved in poliovirus attachment.

    abstract：:A combined assay consisting of a pre-cpe-protection assay and a double-antibody sandwich ELISA for detecting poliovirus was developed on a microtiter scale in order to quantify inhibition of virus replication caused by cell protective antibodies. The system was of high sensitivity and allowed the measurement of the pr...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(91)90080-j

    authors： Barnert RH,Zeichhardt H,Habermehl KO

    更新日期：1991-11-01 00:00:00

  • Isolation of HIV-1 RNA from plasma: evaluation of seven different methods for extraction (part two).

    abstract：:Some new commercial methods for the extraction of viral RNA have been introduced recently. In addition to the study published previously (Verhofstede, C., Reniers, S., Van Wanzeele. F., Plum J., 1996. AIDS 8, 1421-1427), seven different methods (four newly developed and three reference methods) for extraction of HIV-1...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(98)00115-3

    authors： Fransen K,Mortier D,Heyndrickx L,Verhofstede C,Janssens W,van der Groen G

    更新日期：1998-12-01 00:00:00

  • Confirmation of a new conserved linear epitope of Lyssavirus nucleoprotein.

    abstract：:Bioinformatics analysis was used to predict potential epitopes of Lyssavirus nucleoprotein and highlighted some distinct differences in the quantity and localization of the epitopes disclosed by epitope analysis of monoclonal antibodies against Lyssavirus nucleoprotein. Bioinformatics analysis showed that the domain c...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2012.02.008

    authors： Xinjun L,Xuejun M,Lihua W,Hao L,Xinxin S,Pengcheng Y,Qing T,Guodong L

    更新日期：2012-05-01 00:00:00

  • Assessment of template quality by the incorporation of an internal control into a RT-PCR for the detection of rabies and rabies-related viruses.

    abstract：:A method is described to assess RNA template quality by the incorporation of a ribosomal RNA (rRNA) internal (in tube) control into a standard rabies and rabies-related virus specific RT-PCR. Specific virus and rRNA templates were co-amplified in a duplex reaction from RNA extracts derived from 60 isolates representin...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(99)00124-x

    authors： Smith J,McElhinney LM,Heaton PR,Black EM,Lowings JP

    更新日期：2000-02-01 00:00:00

  • A simple and rapid immunochromatographic strip test for monitoring antibodies to H5 subtype Avian Influenza Virus.

    abstract：:A colloid gold strip (CGS) test for detecting antibodies to H5 subtype Avian Influenza Virus (AIV) was developed. The test is based on membrane chromatography and uses colloidal gold conjugated with inactivated AIV-H5N1 as the tracer. On the test strip, a baculovirus expressed haemagglutinin (HA) protein was used as t...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2008.06.011

    authors： Cui S,Chen C,Tong G

    更新日期：2008-09-01 00:00:00