Abstract：
:A new strategy for the simultaneous detection of plant viruses by molecular hybridization has been developed. Two, four or six viral sequences were fused in tandem and transcribed to render unique riboprobes and designated as 'polyprobes'. The 'polyprobe four' (poly 4) covered the four ilarviruses affecting stone fruit trees including apple mosaic virus (ApMV), prunus necrotic ringspot virus (PNRSV), prune dwarf virus (PDV), and American plum line pattern virus (APLPV) whereas the 'polyprobe two' (poly 2) was designed to detect simultaneously, plum pox virus (PPV) and apple chlorotic leaf spot virus (ACLSV), the two more important viruses affecting these trees. Finally, a 'polyprobe six' (poly 6) was generated to detect any of the six viruses. The three polyprobes were comparable to the individual riboprobes in terms of end-point dilution limit and specificity. The validation of the new simultaneous detection strategy was confirmed by the analysis of 46 field samples from up to seven different hosts collected from 10 different geographical areas.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Herranz MC,Sanchez-Navarro JA,Aparicio F,Pallás Vdoi
10.1016/j.jviromet.2004.11.003subject
Has Abstractpub_date
2005-03-01 00:00:00pages
49-55issue
1-2eissn
0166-0934issn
1879-0984pii
S0166-0934(04)00336-2journal_volume
124pub_type
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