Abstract：
:A pandemic caused by a novel influenza A virus (H1N1) poses a serious public health threat. In this study, a real-time reverse transcriptase PCR (RT-PCR) assay based on the hemagglutinin gene was developed that discriminates the novel H1N1 from swine influenza virus, seasonal H1N1/H3N2 virus and the highly pathogenic H5N1 avian influenza virus. The sensitivity of this assay was 0.2 50% tissue culture infective dose of virus and 200 copies of in vitro-transcribed target RNA. Three hundred and forty-eight clinical specimens from suspected H1N1 patients were tested using this assay, and forty-two (12.07%) were found to be positive. Tests using the real-time PCR assay recommended by WHO and virus isolation gave identical results. This sensitive and specific real-time RT-PCR assay will contribute to the early diagnosis and control of the emerging H1N1 influenza pandemic.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Jiang T,Kang X,Deng Y,Zhao H,Li X,Yu X,Yu M,Qin E,Zhu Q,Yang Y,Qin Cdoi
10.1016/j.jviromet.2009.09.021subject
Has Abstractpub_date
2010-02-01 00:00:00pages
470-3issue
2eissn
0166-0934issn
1879-0984pii
S0166-0934(09)00421-2journal_volume
163pub_type
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