Abstract：
:Vaccine manufacturing requires constant analytical monitoring to ensure reliable quality and a consistent safety profile of the final product. Concentration and bioactivity of active components of the vaccine are key attributes routinely evaluated throughout the manufacturing cycle and for product release and dosage. In the case of live attenuated virus vaccines, bioactivity is traditionally measured in vitro by infection of susceptible cells with the vaccine followed by quantification of virus replication, cytopathology or expression of viral markers. These assays are typically multi-day procedures that require trained technicians and constant attention. Considering the need for high volumes of testing, automation and streamlining of these assays is highly desirable. In this study, the automation and streamlining of a complex infectivity assay for Varicella Zoster Virus (VZV) containing test articles is presented. The automation procedure was completed using existing liquid handling infrastructure in a modular fashion, limiting custom-designed elements to a minimum to facilitate transposition. In addition, cellular senescence data provided an optimal population doubling range for long term, reliable assay operation at high throughput. The results presented in this study demonstrate a successful automation paradigm resulting in an eightfold increase in throughput while maintaining assay performance characteristics comparable to the original assay.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Joelsson D,Gates IV,Pacchione D,Wang CJ,Bennett PS,Zhang Y,McMackin J,Frey T,Brodbeck KC,Baxter H,Barmat SL,Benetti L,Bodmer JLdoi
10.1016/j.jviromet.2010.01.016subject
Has Abstractpub_date
2010-06-01 00:00:00pages
1-11issue
1-2eissn
0166-0934issn
1879-0984pii
S0166-0934(10)00017-0journal_volume
166pub_type
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