Structural and biological properties of Cucumber mosaic virus particles carrying hepatitis C virus-derived epitopes.


:The Cucumber mosaic virus (CMV) is a three-component isodiametric plant virus with an extremely wide host range, present worldwide. A pseudorecombinant form has been described, deriving from the RNA3 component of the CMV-S strain, carrying the coat protein (CP) gene, and the RNA 1, 2 components of the CMV-D strain. The CP gene was then engineered to express one or two copies of a synthetic peptide derived from many hypervariable region 1 (HVR1) sequences of the Hepatitis C virus (HCV) envelope protein E2 (the so-called R9 mimotope). Study of the symptoms pattern displayed in tobacco by these chimeric CMV particles, together with determination of their structural characteristics, assessed by circular dichroism (CD) spectroscopy and electron microscopy, revealed a possible relationship between the biological behavior and the structural properties of virus components.


J Virol Methods


Nuzzaci M,Bochicchio I,De Stradis A,Vitti A,Natilla A,Piazzolla P,Tamburro AM




Has Abstract


2009-02-01 00:00:00














  • A quantitative assay for measuring human foamy virus using an established indicator cell line.

    abstract::In order to improve the accuracy for detecting human foamy virus (HFV), an indicator cell line was established by co-transfecting baby hamster kidney-21 cells with two plasmids: one containing a G418 antibiotic resistance marker and the other including the luc gene which was placed downstream of the inducible HFV long...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Tai HY,Sun KH,Kung SH,Liu WT

    更新日期:2001-05-01 00:00:00

  • HPV antibody detection by ELISA with capsid protein L1 fused to glutathione S-transferase.

    abstract::An alternative enzyme linked immunosorbent assay (ELISA) system was developed to analyze antibodies to human papillomavirus capsid antigens. The assay uses glutathione crosslinked to casein to capture the major capsid protein L1 from human papillomavirus (HPV) types 6b, 16 and 18 fused to glutathione S-transferase (GS...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Sehr P,Müller M,Höpfl R,Widschwendter A,Pawlita M

    更新日期:2002-10-01 00:00:00

  • Validation of a modified commercial assay for the detection of rubella-specific IgG in oral fluid for use in population studies.

    abstract::Matching serum and oral fluid (saliva) samples were collected from 369 subjects in Tunisia in 2002, from a city in the north and a rural district in the south. Rubella-specific IgG was detected in sera by commercial ELISA (Dade Behring) and in matching oral fluids by two methods, a previously described IgG-capture ELI...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Ben Salah A,Zaâtour A,Pomery L,Cohen BJ,Brown DW,Andrews N

    更新日期:2003-12-01 00:00:00

  • Analytical evaluation of the microarray-based FluChip-8G Influenza A+B Assay.

    abstract:BACKGROUND:Influenza causes a significant annual disease burden, with characterization of the infecting virus important in clinical and public health settings. Rapid immunoassays are fast but insensitive, whereas real-time RT-PCR is sensitive but susceptible to genetic mutations and often requires multiple serial assay...

    journal_title:Journal of virological methods

    pub_type: 杂志文章,多中心研究


    authors: Taylor AW,Dawson ED,Blair RH,Johnson JE Jr,Slinskey AH,Smolak AW,Toth E,Liikanen K,Stoughton RS,Smith C,Talbot S,Rowlen KL

    更新日期:2019-11-01 00:00:00

  • Rapid molecular detection of Lujo virus RNA.

    abstract::Lujo virus is an emerging arenavirus circulating in Southern Africa. Although to date there has only been a single outbreak of the novel haemorrhagic disease resulting from human infection with this virus, the case-fatality rate of exposed individuals, including nosocomial transmission, was 80%. The ability to identif...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Atkinson B,Chamberlain J,Dowall SD,Cook N,Bruce C,Hewson R

    更新日期:2014-01-01 00:00:00

  • Relationship between plant virus concentration and infectivity: a 'growth curve' model.

    abstract::A logistic ('growth curve') model is formulated and applied to the relationship between numbers of infections (local lesions) produced by a virus on inoculated plants and the concentration of the virus in the inoculum. This model has the advantages of being simple, data-based and therefore not founded on limiting post...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Gokhale DV,Bald JG

    更新日期:1987-12-01 00:00:00

  • A cornucopia of screening and diagnostic techniques for human papillomavirus associated cervical carcinomas.

    abstract::Cervical carcinoma is one of the major consequences of human papillomavirus (HPV) infections. Although HPV infections of cervix do not always progress to cancer, 90% cases of cervical cancer have been found associated with high risk HPV (hrHPV) infection. Usually, HPV infection is asymptomatic; however, this asymptoma...

    journal_title:Journal of virological methods

    pub_type: 杂志文章,评审


    authors: Ramzan M,Noor ul Ain,Ilyas S,Umer M,Bano S,Sarwar S,Shahzad N,Shakoori AR

    更新日期:2015-09-15 00:00:00

  • A non-radioisotopic reverse transcriptase assay using biotin-11-deoxyuridinetriphosphate on primer-immobilized microtiter plates.

    abstract::We developed a non-radioisotopic (non-RI) reverse transcriptase assay (RTA). The reverse transcriptase (RT) incorporates biotin-11-deoxyuridine-triphosphate (bio-dUTP) using a poly(rA) template hybridized with oligo(dT) primer that is immobilized on the surface of a 96-well microtiter plate. This assay is thus semi-au...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Urabe T,Sano K,Tanno M,Mizoguchi J,Otani M,Lee MH,Takasaki T,Kusakabe H,Imagawa DT,Nakai M

    更新日期:1992-11-01 00:00:00

  • A synthetic parvovirus B19 capsid protein can replace viral antigen in antibody-capture enzyme immunoassays.

    abstract::To establish a renewable source of parvovirus B19 antigens for diagnostic tests, gene sequences for the viral capsid proteins, VP1 and VP2, were cloned into baculovirus expression vectors and the recombinant viruses used to infect Sf9 insect cells. Cell lysates examined by immunoblotting demonstrated reactive proteins...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Kock WC

    更新日期:1995-09-01 00:00:00

  • A comparison of PCR assays for beak and feather disease virus and high resolution melt (HRM) curve analysis of replicase associated protein and capsid genes.

    abstract::Beak and feather disease virus (BFDV) threatens a wide range of endangered psittacine birds worldwide. In this study, we assessed a novel PCR assay and genetic screening method using high-resolution melt (HRM) curve analysis for BFDV targeting the capsid (Cap) gene (HRM-Cap) alongside conventional PCR detection as wel...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Das S,Sarker S,Ghorashi SA,Forwood JK,Raidal SR

    更新日期:2016-11-01 00:00:00

  • Development and evaluation of a line probe assay for rapid typing of influenza viruses and detection of the H274Y mutation.

    abstract::Adequate treatment of influenza requires identification of viral type as well as detection of mutation(s) conferring drug resistance. Reverse hybridization-based line probe assays (LiPA) can be performed using several probes immobilized on nitrocellulose, strips enabling LiPA to determine simultaneously viral subtypes...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Miyoshi-Akiyama T,Akasaka Y,Oogane T,Kondo Y,Matsushita T,Funatogawa K,Kirikae T

    更新日期:2012-11-01 00:00:00

  • Diagnosis of canine coronavirus infection using nested-PCR.

    abstract::The results of polymerase chain reaction (PCR) and nested polymerase chain reaction (n-PCR) assays for the diagnosis of canine coronavirus (CCV) infection, and the comparison with other diagnostic techniques, such as electron microscopy (EM) and virus isolation using A-72 cell line are reported. The study was carried ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Pratelli A,Buonavoglia D,Martella V,Tempesta M,Lavazza A,Buonavoglia C

    更新日期:2000-01-01 00:00:00

  • Selection and characterization of single-chain recombinant antibodies against infectious haematopoietic necrosis virus from mouse phage display library.

    abstract::Six single-chain fragment variable (scFv) antibodies against infectious haematopoietic necrosis virus (IHNV) were selected from an antibody phage display library by phage display technology. The soluble scFv antibodies showed a molecular weight 32kDa by Western blot. Dot blot analysis revealed that the six scFv antibo...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Liu H,Zheng X,Shi X,Yu L,Jia P,Wang J,He J,Lan W,Liu H,Wu Z

    更新日期:2014-09-01 00:00:00

  • An indirect haemadsorption procedure (MRSPAH) for detecting antibodies to respiratory syncytial virus.

    abstract::A simple haemadsorption procedure for the detection of isotype specific antibodies to R.S. virus has been developed. Correlation between detection of R.S. virus IgG antibodies by mixed reverse (solid phase) passive antiglobulin haemadsorption (MRSPAH) and other serological procedures has been obtained. IgA antibody wa...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Cranage MP,Coombs RR

    更新日期:1982-11-01 00:00:00

  • Competitive polymerase chain reaction assay for quantitation of HIV-1 DNA and RNA.

    abstract::A quantitative PCR assay for the detection of HIV-1 nucleic acids is described. The assay is based on a competitive internal standard nucleic acid which can be discriminated from target sequences by the presence of a new restriction enzyme site. The method was used to quantitate plasmid molecules containing HIV-1 sequ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Stieger M,Démollière C,Ahlborn-Laake L,Mous J

    更新日期:1991-09-01 00:00:00

  • Demonstration of rabbit haemorrhagic disease virus antigen by Staphylococcus protein A coagglutination test.

    abstract::The Staphylococcus protein A coagglutination test (Sp A COAT) was developed for the diagnosis of rabbit haemorrhagic disease (RHD). Liver, spleen, lungs and kidneys from 176 rabbits dead from viral haemorrhagic disease as well as the same organs from 64 healthy animals were examined by Sp A COAT and haemaglutination t...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Peshev R,Alexandrov M,Ivanov Y,Gogov Y

    更新日期:1996-03-01 00:00:00

  • Optimization of in situ cellular ELISA performed on influenza A virus-infected monolayers for screening of antiviral agents.

    abstract::Viral susceptibility testing has been traditionally performed by the plaque reduction assay (PRA) which is laborious, time consuming, relatively expensive, and requires subjective input by the reader. An in situ cellular enzyme-linked immunosorbent assay (ELISA) has been developed with the potential to overcome many o...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Myc A,Anderson MJ,Baker JR Jr

    更新日期:1999-02-01 00:00:00

  • Field detection of avian influenza virus in wild birds: evaluation of a portable rRT-PCR system and freeze-dried reagents.

    abstract::Wild birds have been implicated in the spread of highly pathogenic avian influenza (HPAIV) of the H5N1 subtype, prompting surveillance along migratory flyways. Sampling of wild birds is often conducted in remote regions, but results are often delayed because of limited local analytical capabilities, difficulties with ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Takekawa JY,Iverson SA,Schultz AK,Hill NJ,Cardona CJ,Boyce WM,Dudley JP

    更新日期:2010-06-01 00:00:00

  • Comparative study of the replication of infectious bursal disease virus in DF-1 cell line and chicken embryo fibroblasts evaluated by a new real-time RT-PCR.

    abstract::A real-time RT-PCR method was developed for the detection of infectious bursal disease virus (IBDV). The VP5 gene of IBDV was chosen as the target binding region for a specific TaqMan probe. The results showed that viral genomic copy number could be quantified accurately ranging from 10(8)copies/microL to 10(1)copies/...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Wang Y,Qi X,Gao H,Gao Y,Lin H,Song X,Pei L,Wang X

    更新日期:2009-05-01 00:00:00

  • Isolation of mutants of Aujeszky's disease virus with antigenically altered glycoprotein E by affinity chromatography using monoclonal antibodies.

    abstract::An efficient method for isolation of virus mutants with antigenically altered proteins is described. The method is based on the separation of viruses with wild-type and antigenically altered proteins by affinity chromatography using monoclonal antibodies (MAbs). A nonessential glycoprotein E (gE) of Aujeszky's disease...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Morenkov OS,Smirnov SV,Vrublevskaya VV

    更新日期:1999-01-01 00:00:00

  • An alternative method of measuring aerosol survival using spiders' webs and its use for the filoviruses.

    abstract::Understanding the ability to survive in an aerosol leads to better understanding of the hazard posed by pathogenic organisms and can inform decisions related to the control and management of disease outbreaks. This basic survival information is sometimes lacking for high priority select agents such as the filoviruses ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Smither SJ,Piercy TJ,Eastaugh L,Steward JA,Lever MS

    更新日期:2011-10-01 00:00:00

  • A fluorescence-based quantitative PCR method for investigation of pseudorabies virus latency.

    abstract::A quantitative PCR method was developed in order to quantitate the number of copies of Pseudorabies virus (PRV) genome present in tissues from infected pigs. The method is based on the use of an internal standard that differs from the target DNA by a deletion of ten base pairs, and that is co-amplified with the target...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Thiery R,Pannetier C,Rziha HJ,Jestin A

    更新日期:1996-09-01 00:00:00

  • TaqMan real-time PCR for detection and quantitation of squash leaf curl virus in cucurbits.

    abstract::A real-time PCR assay based on the TaqMan chemistry was developed for reliable detection and quantitation of the squash leaf curl virus (SLCV) in melon and squash plants. This method was highly specific to SLCV and it was about one thousand times more sensitive than the conventional PCR method. The protocol of the rea...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Kuan CP,Huang HC,Chang CC,Lu YL

    更新日期:2012-02-01 00:00:00

  • Baculovirus expressed 2C of foot-and-mouth disease virus has the potential for differentiating convalescent from vaccinated animals.

    abstract::Determining whether animals have been infected with foot-and-mouth disease virus or vaccinated is important because infected animals frequently become carriers of the virus, shed it intermittently and thus may be the source of new outbreaks of the disease. We had shown previously that the sera of convalescent animals ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Meyer RF,Babcock GD,Newman JF,Burrage TG,Toohey K,Lubroth J,Brown F

    更新日期:1997-04-01 00:00:00

  • Solid-phase enzyme immunoassay for rotavirus antigen: faecal protease activity as a reason for false-negative results.

    abstract::Rabbits and guinea pigs were immunized with purified bovine rotavirus. Immunoglobulin G fractions of the resulting antisera were used in a standard four-layer solid-phase enzyme immunoassay (EIA) for rotavirus antigen in human faecal specimens. Samples negative for rotavirus in electron microscopy, when diluted in sta...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Hovi T,Väisänen V,Ukkonen P,von Bonsdorff CH

    更新日期:1982-09-01 00:00:00

  • Development of a recombinant yellow fever vector expressing a HIV clade C founder envelope gp120.

    abstract::Development of a HIV-1 vaccine is a major global priority. The yellow fever virus (YFV) attenuated vaccine 17D is among the most effective of currently used vaccines. However, the stability of the YFV17D vector when carrying non-flavivirus genes has been problematic. We have constructed and expressed HIV-1 Env in YFV1...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Yu JS,Liao HX,Pritchett J,Bowman C,Vivian C,Parks R,Xia SM,Cooper M,Williams WB,Bonsignori M,Reed SG,Chen M,Vandergrift N,Rice CM,Haynes BF

    更新日期:2017-11-01 00:00:00

  • Comparison of specific and random priming in the reverse transcriptase polymerase chain reaction for genotyping group A rotaviruses.

    abstract::This study describes an approach to the molecular typing of rotaviruses which requires only a single RNA extraction and reverse transcription (RT) reaction using random primers. Random-primed RT provides complementary DNA (cDNA) which can be used not only for G- and P-typing polymerase chain reactions (PCR), but also ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Iturriza-Gomara M,Green J,Brown DW,Desselberger U,Gray JJ

    更新日期:1999-03-01 00:00:00

  • Predicting whole genome sequencing success for archived avian influenza virus (Orthomyxoviridae) samples using real-time and droplet PCRs.

    abstract::Long-term viral archives are valuable sources of research data. Each archive can store hundreds of thousands of diverse sample types. In the current era of whole genome sequencing, archived samples become a rich source of evolutionary and epidemiological data that can span years, and even decades. However, the ability...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Hopken MW,Piaggio AJ,Pabilonia KL,Pierce J,Anderson T,Abdo Z

    更新日期:2020-02-01 00:00:00

  • Comparison of hemagglutination inhibition assay, an ELISA-based micro-neutralization assay and colorimetric microneutralization assay to detect antibody responses to vaccination against influenza A H1N1 2009 virus.

    abstract::The hemagglutination inhibition (HI) assay has been the main method used to investigate immune responses to vaccination against influenza H1N1 (2009) virus. However microneutralization tests (MNT) have been shown to be more sensitive and more specific. In this study, the three methods of choice: (i) the HI assay, (ii)...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Grund S,Adams O,Wählisch S,Schweiger B

    更新日期:2011-02-01 00:00:00

  • Application of the heteroduplex mobility assay (HMA) for the investigation of the genomic diversity among noroviruses in environmental samples.

    abstract::Recent studies have demonstrated the widespread contamination of river and seawater with noroviruses (NV), often with more than one strain. The heteroduplex mobility assay (HMA) in which amplicons from study samples are hybridised (by denaturing and reannealing) to amplicons from reference strains and resolved by elec...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Green J,Gallimore CI,Shore J,Sellwood J,Brown DW

    更新日期:2004-09-01 00:00:00