Structural and biological properties of Cucumber mosaic virus particles carrying hepatitis C virus-derived epitopes.

Abstract:

:The Cucumber mosaic virus (CMV) is a three-component isodiametric plant virus with an extremely wide host range, present worldwide. A pseudorecombinant form has been described, deriving from the RNA3 component of the CMV-S strain, carrying the coat protein (CP) gene, and the RNA 1, 2 components of the CMV-D strain. The CP gene was then engineered to express one or two copies of a synthetic peptide derived from many hypervariable region 1 (HVR1) sequences of the Hepatitis C virus (HCV) envelope protein E2 (the so-called R9 mimotope). Study of the symptoms pattern displayed in tobacco by these chimeric CMV particles, together with determination of their structural characteristics, assessed by circular dichroism (CD) spectroscopy and electron microscopy, revealed a possible relationship between the biological behavior and the structural properties of virus components.

journal_name

J Virol Methods

authors

Nuzzaci M,Bochicchio I,De Stradis A,Vitti A,Natilla A,Piazzolla P,Tamburro AM

doi

10.1016/j.jviromet.2008.10.005

subject

Has Abstract

pub_date

2009-02-01 00:00:00

pages

118-21

issue

2

eissn

0166-0934

issn

1879-0984

pii

S0166-0934(08)00363-7

journal_volume

155

pub_type

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