Abstract:
:To investigate various aspects of the latency of pseudorabies virus in swine (PRV, suid herpesvirus 1) we developed in vitro nucleic acid amplification methods based upon the polymerase chain reaction. Primers flanking a 156-bp region of the pseudorabies virus gp II gene were annealed to purified PRV DNA as well as DNA isolated from the trigeminal ganglia of swine latently infected with PRV and subjected to PCR amplification. Following amplification, 100 fg of PRV DNA was visualizable on stained gels and 1 fg (equivalent to 6 viral genome copies) was detectable when amplification was combined with blot hybridization. PRV-specific DNA sequences which remained undetectable by direct blot hybridization assays were amplified to levels visualizable on ethidium-bromide-stained gels in 5 of 5 experimental latently infected animals. In addition, oligonucleotide primers specific for a 223-bp region of the PRV immediate-early gene (IE 180) were capable of amplifying overlapping latency associated transcripts (LATs), via a cDNA intermediate, in 6 of 6 latently infected swine. These nucleic acid amplification methods should be applicable to the investigation of PRV latency, and gene expression during latency and reactivation, in which few cells harbor latent virus.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Lokensgard JR,Thawley DG,Molitor TWdoi
10.1016/0166-0934(91)90120-osubject
Has Abstractpub_date
1991-09-01 00:00:00pages
45-55issue
1eissn
0166-0934issn
1879-0984pii
0166-0934(91)90120-Ojournal_volume
34pub_type
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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abstract::Hepatitis B-virucidal testing of biocides in quantitative suspension tests using duck hepatitis B virus (DHBV) requires primary duck embryonic hepatocytes for viral propagation. To improve the test system and availability of these cells, commercial culture plates with different growth surfaces were tested for cell cul...
journal_title:Journal of virological methods
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journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/0166-0934(87)90007-3
更新日期:1987-06-01 00:00:00
abstract::A virus (PBCV) from the symbiotic Chlorella-like algae of Paramecium bursaria has been characterized by sedimentation field flow fractionation (SdFFF). The method yields effective molecular weight M' (molecular weight X buoyancy factor) with a precision of better than 5% under a variety of experimental conditions. The...
journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/0166-0934(85)90038-2
更新日期:1985-06-01 00:00:00
abstract:BACKGROUND:Molecular assays for diagnosis of Flu A, Flu B, and RSV with short turn-around-time (TAT) are of considerable clinical importance. In addition, rapid and accurate diagnosis of a large panel of viral and atypical pathogens can be crucial in immunocompromised patients. OBJECTIVES:First, to evaluate the perfor...
journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/j.jviromet.2017.03.013
更新日期:2017-07-01 00:00:00
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/0166-0934(84)90055-7
更新日期:1984-12-01 00:00:00
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journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/j.jviromet.2005.05.011
更新日期:2005-09-01 00:00:00
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journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/j.jviromet.2013.08.012
更新日期:2013-12-01 00:00:00
abstract::No effective prophylactic or therapeutic vaccine against HIV-1 in humans is currently available. This study analyzes the immunogenicity and safety of a recombinant attenuated vaccinia virus. A chimeric gene of HIV-1 multi-epitope genes containing CpG ODN and cholera toxin B subunit (CTB) was inserted into Chinese vacc...
journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/j.jviromet.2015.03.007
更新日期:2015-07-01 00:00:00
abstract::Shrimp farming is one of the most important aquaculture activities. Expansion and intensification of shrimp farming has been accompanied with the outbreak of diseases, which threaten the development and sustainability of the industry. Viral diseases are the major challenges faced by shrimp farming industries. The prev...
journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/j.jviromet.2014.05.006
更新日期:2014-09-01 00:00:00
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journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/0166-0934(92)90161-6
更新日期:1992-01-01 00:00:00
abstract::HPV-16 L1 methylation and E6/E7 mRNA have suggested that they had close relationship with cervical neoplastic progression. This study aimed to evaluate the clinical performance of the HPV-16 L1 methylation assay and E6/E7 mRNA test for detecting high-grade cervical lesions (CIN2+). A total of 81 women with liquid-base...
journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/j.jviromet.2015.08.008
更新日期:2015-11-01 00:00:00
abstract::Commercially marketed kits are now available for rapid viral assay of clinical specimens. This study was conducted to determine the suitability of these kits for use in environmental testing. Eight rotavirus kits and one enteric adenovirus kit were screened for sensitivity using simian rotavirus SA11, human rotavirus ...
journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/0166-0934(93)90098-c
更新日期:1993-12-15 00:00:00
abstract::The first generation of proprietary reagents for detecting antibodies to the Human Immunodeficiency Virus Type 1 (HIV-1) by enzyme-linked immunosorbent assay (ELISA) used as antigen partially purified virus from cell culture lysates. These tests, which are still in use, may vary in their antibody measurement capabilit...
journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/0166-0934(92)90028-c
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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更新日期:1993-10-01 00:00:00
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journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/0166-0934(88)90131-0
更新日期:1988-08-01 00:00:00
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journal_title:Journal of virological methods
pub_type: 杂志文章
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journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/0166-0934(87)90011-5
更新日期:1987-07-01 00:00:00
abstract::A rapid and efficient method is described for isotype quantitation of antiviral antibodies in mice infected with Theiler's murine encephalomyelitis virus (TMEV). Serum antibodies were reacted with fluorochrome-labeled TMEV in a modified fluid-phase particle concentration fluorescence immunoassay (PCFIA). Biotin and av...
journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/0166-0934(90)90135-3
更新日期:1990-02-01 00:00:00
abstract::A large number of Norwalk-like viruses (NLVs) have been identified from stool samples by RT-PCR by amplifying part of the polymerase-coding gene. A set of probes were selected based on sequence analysis of the viruses circulating in Finland during the years 1996-97 for confirmation of the findings by hybridization. A ...
journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/s0166-0934(99)00115-9
更新日期:1999-12-01 00:00:00
abstract:OBJECTIVE:This study evaluated the performance of the cobas® hepatitis C virus (HCV) Test for use on the cobas® 6800/8800 Systems for the detection and quantification of HCV RNA collected using the cobas® Plasma Separation Card (PSC) compared with plasma samples. METHODS:Whole EDTA-venous blood was collected from 50 H...
journal_title:Journal of virological methods
pub_type: 杂志文章
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更新日期:2020-04-01 00:00:00
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journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/0166-0934(93)90091-5
更新日期:1993-06-01 00:00:00
abstract::An international collaborative study was performed to evaluate a set of PCR reference reagents for HIV diagnosis. Twenty-six laboratories from 9 countries analysed a proficiency panel of 10 coded DNA samples using the PCR reference reagents and protocols. For comparison, these coded samples were then assessed using a ...
journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/0166-0934(92)90018-9
更新日期:1992-04-01 00:00:00
abstract::Rice stripe virus (RSV), Rice black-streaked dwarf virus (RBSDV), and Rice dwarf virus (RDV) are major rice-infecting viruses in Korea that can cause serious crop losses. A one-step multiplex reverse transcription-polymerase chain reaction (mRT-PCR) was developed for the simultaneous detection of these rice viruses. T...
journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/j.jviromet.2013.07.008
更新日期:2013-11-01 00:00:00