Serotype-specific detection of adeno-associated virus during laboratory preparation.

Abstract:

:Adeno-associated virus (AAV) is a small single-stranded DNA member of the family Parvoviradae with at least eight recognized human serotypes, several of which are being studied as candidate vectors for gene therapy. When multiple serotypes are handled in the same laboratory, it is critical to know the serotype of a sample with certainty. Here, a rapid and reliable PCR-based method is presented for the identification of serotypes 2, 3B or 6 and for screening for cross contamination. The PCR assay is based on the use of differentially annealing, serotype-specific primer pairs targeted to the hypervariable regions of the capsid coding region of the AAV genome. Identity is determined by the presence of a PCR product of size specific to each serotype. Multiplexing the reaction allows all serotypes to be queried in a single reaction tube and greatly diminishes the presence of false positives. The method is capable of detecting as little as 25 fg of a contaminating serotype and is potentially extensible to other AAV serotypes.

journal_name

J Virol Methods

authors

Mitchell DA,O'Donnell J,Hare JT,Chapman MS

doi

10.1016/j.jviromet.2006.05.012

subject

Has Abstract

pub_date

2006-09-01 00:00:00

pages

277-82

issue

1-2

eissn

0166-0934

issn

1879-0984

pii

S0166-0934(06)00174-1

journal_volume

136

pub_type

杂志文章
  • Mass spectrometric identification and characterisation of the nucleocapsid protein of Menangle virus.

    abstract::The recent emergence of novel viruses requires reliable methodology for their identification and confirmation both on a cellular and molecular level. Mass spectrometry offers a suitable approach for the identification and characterisation of viral proteins and its application is demonstrated in this study. Menangle vi...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/s0166-0934(01)00441-4

    authors: Shiell BJ,Beddome G,Michalski WP

    更新日期:2002-04-01 00:00:00

  • Detection of rhinovirus and enterovirus in upper respiratory tract samples using a multiplex nested PCR.

    abstract::Human enteroviruses and rhinoviruses are respiratory pathogens whose role in acute respiratory infection is underestimated due to the use of diagnostic procedures with poor sensitivity. To determine the prevalence of these two pathogens in the upper respiratory tract infections, a multiplex procedure was developed tha...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/s0166-0934(03)00038-7

    authors: Billaud G,Peny S,Legay V,Lina B,Valette M

    更新日期:2003-03-01 00:00:00

  • DNA: DNA hybridization method for the diagnosis of hepatitis B infection.

    abstract::Hepatitis B viral (HBV) DNA was detected in a hepatoma cell line which produces hepatitis B surface antigen (HBsAg) and in patients with acute hepatitis B. The serum of one patient with acute hepatitis B was found to be infectious when injected i.v. into a chimpanzee up to a dilution of 10(-8). Hepatitis B surface ant...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/0166-0934(84)90014-4

    authors: Feinman SV,Berris B,Guha A,Sooknanan R,Bradley DW,Bond WW,Maynard JE

    更新日期:1984-05-01 00:00:00

  • In vitro replication of Macrobrachium rosenbergii nodavirus and extra small virus in C6/36 mosquito cell line.

    abstract::White tail disease (WTD) is a serious problem in hatcheries and nursery ponds of Macrobrachium rosenbergii in India and many parts of the world. The pathogenic agents have been identified as M. rosenbergii nodavirus (MrNV) associated with extra small virus (XSV), which is 27nm and 15nm in diameter, respectively. Repli...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2007.06.011

    authors: Sudhakaran R,Parameswaran V,Sahul Hameed AS

    更新日期:2007-12-01 00:00:00

  • A generic real-time TaqMan assay for specific detection of lapinized Chinese vaccines against classical swine fever.

    abstract::Classical swine fever (CSF) is a highly contagious disease, causing severe economic losses in the pig industry worldwide. Vaccination of pigs with lapinized Chinese vaccines is still practised in some regions of the world, where the virus is enzootic, in order to prevent and control the disease. However, a single real...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2011.05.003

    authors: Liu L,Xia H,Everett H,Sosan O,Crooke H,Meindl-Böhmer A,Qiu HJ,Moennig V,Belák S,Widén F

    更新日期:2011-08-01 00:00:00

  • Determination of specific cytomegalovirus IgM antibodies using infected air dried cells and isolated nuclei by immunoperoxidase assay.

    abstract::A simple immunoperoxidase assay (IPA), adapted for detection of serum IgM antibodies to cytomegalovirus (CMV) is described. The antigen consisted of CMV infected human embryonic fibroblasts or isolated nuclei. The sera were absorbed with aggregated gamma-globulins prior to testing. Rabbit anti-human IgM peroxidase con...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/0166-0934(85)90122-3

    authors: Singer Y,Kimmel N,Sarov I

    更新日期:1985-05-01 00:00:00

  • Expression and the antigenicity of recombinant coat proteins of tungro viruses expressed in Escherichia coli.

    abstract::Rice tungro disease (RTD) is a recurring disease affecting rice farming especially in the South and Southeast Asia. The disease is commonly diagnosed by visual observation of the symptoms on diseased plants in paddy fields and by polymerase chain reaction (PCR). However, visual observation is unreliable and PCR can be...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2016.12.001

    authors: Yee SF,Chu CH,Poili E,Sum MSH

    更新日期:2017-02-01 00:00:00

  • Development and evaluation of a generic tag array to detect and genotype noroviruses in water.

    abstract::Noroviruses are the leading cause of nonbacterial gastroenteritis outbreaks in the United States, some of which are caused by the ingestion of contaminated water. Detection and genotypic characterization of noroviruses is commonly performed by reverse transcription-polymerase chain reaction (RT-PCR) followed by sequen...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2008.03.010

    authors: Brinkman NE,Fout GS

    更新日期:2009-03-01 00:00:00

  • Multiplex PCR for simultaneous detection and differentiation of sheeppox, goatpox and orf viruses from clinical samples of sheep and goats.

    abstract::A multiplex polymerase chain reaction (mPCR) was developed and evaluated for detection of pox viral infections simultaneously using clinical samples from sheep and goats. Specific primers for three pox viruses of sheep and goats including sheeppox virus (SPPV), goatpox virus (GTPV) and orf virus (ORFV) were designed t...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2013.10.009

    authors: Venkatesan G,Balamurugan V,Bhanuprakash V

    更新日期:2014-01-01 00:00:00

  • Genotype-specific fluorogenic RT-PCR assays for the detection and quantitation of canine coronavirus type I and type II RNA in faecal samples of dogs.

    abstract::Two genotype-specific fluorogenic RT-PCR assays were developed for the detection and quantitation of canine coronavirus (CCoV) type I and type II RNA in the faeces of dogs with diarrhoea. Both the fluorogenic assays showed high specificity, sensitivity and reproducibility, allowing a precise quantitation of CCoV type ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2005.06.005

    authors: Decaro N,Martella V,Ricci D,Elia G,Desario C,Campolo M,Cavaliere N,Di Trani L,Tempesta M,Buonavoglia C

    更新日期:2005-12-01 00:00:00

  • Gene transfer into hepatocytes mediated by herpes simplex virus-Epstein-Barr virus hybrid amplicons.

    abstract::Gene transfer into hepatocytes is highly desirable for the long-term goal of replacing deficient proteins and correcting metabolic disorders. Vectors based on herpes simplex virus type-1 (HSV-1) have been demonstrated to mediate efficient gene transfer into hepatocytes both in vitro and in vivo. Large transgene capaci...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2004.09.007

    authors: Müller L,Saydam O,Saeki Y,Heid I,Fraefel C

    更新日期:2005-01-01 00:00:00

  • Multiplication of Taura syndrome virus in primary hemocyte culture of shrimp (Penaeus vannamei).

    abstract::The propagation of Taura syndrome virus (TSV) in primary hemocyte culture of Pacific white shrimp (Penaeus vannamei) was investigated. Purified TSV was inoculated into a 24 h old primary hemocyte culture and the development of cytopathic effects was monitored. The cell morphology started changing within 6 h post-inocu...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2010.12.020

    authors: George SK,Kaizer KN,Betz YM,Dhar AK

    更新日期:2011-03-01 00:00:00

  • A real time RT-PCR assay for the specific detection of Peste des petits ruminants virus.

    abstract::Peste des petits ruminants virus (PPRV) causes a devastating disease of small ruminants present across much of Africa and Asia. Recent surveillance activities and phylogenetic analyses have suggested that the virus is an emerging problem as it is now being detected in areas previously free of the disease. As such, the...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2010.11.022

    authors: Batten CA,Banyard AC,King DP,Henstock MR,Edwards L,Sanders A,Buczkowski H,Oura CC,Barrett T

    更新日期:2011-02-01 00:00:00

  • Inoculation of plants with begomoviruses by particle bombardment without cloning: Using rolling circle amplification of total DNA from infected plants and whiteflies.

    abstract::A new system for inoculation of plants with begomoviral DNA without cloning or the use insect vectors is described. Total DNA extracted from begomovirus-infected plants was amplified by rolling circle amplification (RCA) using the bacteriophage phi29 DNA polymerase, and inoculated to plants by particle bombardment. In...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2010.04.022

    authors: Guenoune-Gelbart D,Sufrin-Ringwald T,Capobianco H,Gaba V,Polston JE,Lapidot M

    更新日期:2010-09-01 00:00:00

  • Disinfection protocols for necropsy equipment in rabies laboratories: Safety of personnel and diagnostic outcome.

    abstract::In the last decades, molecular techniques have gradually been adopted for the rapid confirmation of results obtained through gold standard methods. However, international organisations discourage their use in routine laboratory investigations for rabies post-mortem diagnosis, as they may lead to false positive results...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2016.03.017

    authors: Aiello R,Zecchin B,Tiozzo Caenazzo S,Cattoli G,De Benedictis P

    更新日期:2016-08-01 00:00:00

  • Detection of feline Coronavirus in effusions of cats with and without feline infectious peritonitis using loop-mediated isothermal amplification.

    abstract::Feline infectious peritonitis (FIP) is a fatal disease in cats worldwide. The aim of this study was to test two commercially available reaction mixtures in a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay to detect feline Coronavirus (FCoV) in body cavity effusions of cats with and withou...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2018.03.003

    authors: Günther S,Felten S,Wess G,Hartmann K,Weber K

    更新日期:2018-06-01 00:00:00

  • Improvement of a quantitative cell tropism assay for rapid and reliable characterization of human cytomegalovirus mutants.

    abstract::Endothelial cell tropism is an inherent property of isolates of human cytomegalovirus (HCMV), and has been proposed as a pathogenicity factor of HCMV. Mutational approaches can be applied for analysis of the molecular determinants of endothelial cell tropism, but the evaluation of mutants is limited by the low reliabi...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2010.03.031

    authors: Schuessler A,Sampaio KL,Sinzger C

    更新日期:2010-08-01 00:00:00

  • Easy-to-use rapid gene amplification method for direct detection of RNA and DNA viruses in sera and feces from various animals.

    abstract::The development of rapid and simple gene amplification tests is required for detection of pathogens to prevent transmission of infectious diseases between animals or from animals to humans. An easy-to-use rapid gene amplification method that can directly detect RNA and DNA viruses in clinical samples was developed. Th...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2014.01.019

    authors: Segawa T,Kobayashi Y,Sase Y,Itou T,Suzuki M,Endoh T,Nakanishi T,Sakai T

    更新日期:2014-06-01 00:00:00

  • The influence of incubation time on adenovirus quantitation in A549 cells by most probable number.

    abstract::Cell culture based assays used to detect waterborne viruses typically call for incubating the sample for at least two weeks in order to ensure that all the culturable virus present is detected. Historically, this estimate was based, at least in part, on the length of time used for detecting poliovirus. In this study, ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2016.09.002

    authors: Cashdollar JL,Huff E,Ryu H,Grimm AC

    更新日期:2016-11-01 00:00:00

  • Continuous porcine cell lines developed from alveolar macrophages: partial characterization and virus susceptibility.

    abstract::Porcine monomyeloid cell lines were established following transfection of primary porcine alveolar macrophage cultures with plasmid pSV3neo, carrying genes for neomycin resistance and SV40 large T antigen. The parental clone 3D4 exhibited a relatively rapid doubling time (25.5 h), high plating efficiency and mixed phe...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/s0166-0934(02)00085-x

    authors: Weingartl HM,Sabara M,Pasick J,van Moorlehem E,Babiuk L

    更新日期:2002-07-01 00:00:00

  • Detection of CMV in urine: comparison between DNA-DNA hybridization, virus isolation, and immunoelectron microscopy.

    abstract::Rapid detection of CMV-DNA in urine specimens by dot-blot hybridization was compared to conventional virus isolation and to virus identification using solid-phase immunoelectron microscopy (SPIEM). To detect viral DNA, 32P-labeled EcoR1 J fragment of CMV-DNA was used as a probe in the hybridization assay. In addition,...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/0166-0934(87)90028-0

    authors: Vonsover A,Gotlieb-Stematsky T,Sayar Y,Bardov L,Manor Y,Siegal B

    更新日期:1987-05-01 00:00:00

  • Virus isolation and full-length genome sequencing of a representative canine distemper virus wild type strain of the South America 2 clade.

    abstract::Canine Distemper Virus (CDV) is a highly contagious pathogen of dogs that causes severe respiratory, gastrointestinal and nervous signs. Although vaccines have been used to prevent infections, CDV has been reported worldwide, even in vaccinated animals. In the present study, a representative wild type CDV strain (Arg2...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2020.113857

    authors: Romanutti C,Keller L,La Torre J,Panzera Y,Fuques E,Pérez R,Gallo Calderon M

    更新日期:2020-05-01 00:00:00

  • Generic detection and differentiation of tobamoviruses by a spot nested RT-PCR-RFLP using dI-containing primers along with homologous dG-containing primers.

    abstract::A spot nested RT-PCR-RFLP method to detect and identify all members of the Tobamovirus genus is described. It involves a one-step RT-PCR, in which the combination of degenerate deoxyinosine (dI)-substituted primers amplified part of the polymerase region of tobamoviruses, followed by a nested PCR amplification that in...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2004.01.004

    authors: Dovas CI,Efthimiou K,Katis NI

    更新日期:2004-05-01 00:00:00

  • Characterization of real-time microarrays for simultaneous detection of HIV-1, HIV-2, and hepatitis viruses.

    abstract::Real-time PCR assays for nucleic acid testing (NAT) of hepatitis viruses A-E and for HIV-1 and HIV-2 have been developed; however, a multiplex assay that can simultaneously detect all of these agents is not yet available. Standardized TaqMan assays for detection of hepatitis viruses A-E have been described and applied...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2018.06.001

    authors: Granade TC,Kodani M,Wells SK,Youngpairoj AS,Masciotra S,Curtis KA,Kamili S,Owen SM

    更新日期:2018-09-01 00:00:00

  • Detection and quantitation of group A rotaviruses by competitive and real-time reverse transcription-polymerase chain reaction.

    abstract::A competitive reverse transcription-polymerase chain reaction (RT-PCR) was developed to detect and to quantitate the RNA of group A rotaviruses. In the assay, a 433 bp fragment is amplified by a one-tube RT-PCR protocol using primers with binding sites located in a highly conserved region of segment 6 of the rotavirus...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/s0166-0934(02)00118-0

    authors: Schwarz BA,Bange R,Vahlenkamp TW,Johne R,Müller H

    更新日期:2002-09-01 00:00:00

  • An indirect ELISA for the detection of antibodies against porcine reproductive and respiratory syndrome virus using recombinant nucleocapsid protein as antigen.

    abstract::An indirect enzyme-linked immunosorbent assay termed rnPRRS ELISA using baculovirus-expressed and affinity-purified viral nucleocapsid protein (rNC) antigen was developed for detecting antibodies against porcine reproductive and respiratory syndrome virus (PRRSV) in swine sera. Sera (1395) originating from different E...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/s0166-0934(97)02186-1

    authors: Denac H,Moser C,Tratschin JD,Hofmann MA

    更新日期:1997-05-01 00:00:00

  • Evaluation of currently available bovine viral diarrhoea virus (BVDV) and HoBi-like pestivirus (HoBiPeV) specific diagnostic tests in detection of highly divergent HoBiPeVs in cattle.

    abstract::The emergence of novel and divergent HoBi-like pestivirus (HoBiPeV) strains in cattle in Asia recently has raised concerns with regard to their reliable and accurate diagnosis. Hence, the aim of this study was to evaluate currently available BVDV diagnostic tests and HoBiPeV-specific diagnostic tests in detection of g...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2019.113707

    authors: Moorthy D,Mishra N,Kalaiyarasu S,Jhade SK,Singh VP

    更新日期:2019-10-01 00:00:00

  • Mapping of epitopes of VP2 protein of chicken anemia virus using monoclonal antibodies.

    abstract::To map the epitopes of VP2 protein of chicken anemia virus (CAV), VP2 was expressed as a fusion protein in Escherichia coli BL21 (DE3). The Western blot demonstrated that recombinant VP2 protein could be recognized by sera of chickens infected with CAV. Female BALB/c mice were immunized with purified recombinant VP2 p...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2007.03.016

    authors: Wang X,Gao H,Gao Y,Fu C,Wang Z,Lu G,Cheng Y,Wang X

    更新日期:2007-08-01 00:00:00

  • Generation of a human hepatoma cell line supporting efficient replication of a lamivudine resistant hepatitis B virus.

    abstract::Emergence of lamivudine (LAM) resistance causes treatment failure in patients with chronic hepatitis B and compromise the efficacy of subsequent salvage therapies with other nucleot(s)ide analogs (NAs). Establishment of cell-based assays supporting LAM-resistant hepatitis B virus (HBV) replication will not only provid...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2014.02.008

    authors: Zhang Y,Zhang Y,Kang Y,Wang J,Liu H,Zhu H,Qin Y,Mao R,Lin X,Lu M,Zhang J

    更新日期:2014-06-01 00:00:00

  • Transient replication of a hepatitis C virus genotype 1b replicon chimera encoding NS5A-5B from genotype 3a.

    abstract::Although hepatitis C virus (HCV) is a pathogen of global significance, experimental therapies in current clinical development include highly efficacious all-oral combinations of HCV direct-acting antivirals (DAAs). If approved for use, these new treatment regimens will impact dramatically upon our capacity to eradicat...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2013.09.017

    authors: Kylefjord H,Danielsson A,Sedig S,Belda O,Wiktelius D,Vrang L,Targett-Adams P

    更新日期:2014-01-01 00:00:00