Comparison of hemagglutination inhibition, serum neutralization and ELISA-PVM tests for the detection of antibodies to pneumonia virus of mice in rat sera.

abstract：:Clones isolated from a genomic library of the baculovirus PmSNPV (= MBV) were used to prepare DNA probes for detection of PmSNPV in shrimp tissue. Davidson's AFA fixed shrimp tissues were probed using DNA that had been labeled with the hapten, digoxigenin, by an in situ hybridization assay. The DNA probes readily dist...

journal_title：Journal of virological methods

authors： Poulos BT,Mari J,Bonami JR,Redman R,Lightner DV

更新日期：1994-09-01 00:00:00

abstract：:The Hawaii agent is a Norwalk-like virus of acute gastroenteritis in humans which is antigenically distinct from the prototype Norwalk agent. We established a solid phase sandwich type microtiter enzyme immunoassay (EIA) for Hawaii antigen employing sera and stools from experimentally challenged volunteers as reagents...

journal_title：Journal of virological methods

authors： Treanor JJ,Madore HP,Dolin R

更新日期：1988-12-01 00:00:00

abstract：:Chronic HCV infection is one of the leading causes of liver-related death and in many countries it is a primary reason for having a liver transplant. HCV genotype identification has long been used in the clinical practice, since different genotypes have different response rates and required different doses and duratio...

journal_title：Journal of virological methods

authors： Dirani G,Paesini E,Mascetra E,Farabegoli P,Dalmo B,Bartolini B,Garbuglia AR,Capobianchi MR,Sambri V

更新日期：2018-01-01 00:00:00

abstract：:A colloid gold strip (CGS) test for detecting antibodies to H5 subtype Avian Influenza Virus (AIV) was developed. The test is based on membrane chromatography and uses colloidal gold conjugated with inactivated AIV-H5N1 as the tracer. On the test strip, a baculovirus expressed haemagglutinin (HA) protein was used as t...

journal_title：Journal of virological methods

authors： Cui S,Chen C,Tong G

更新日期：2008-09-01 00:00:00

abstract：:Reverse genetics systems, with the ability to manipulate viral genomes at the DNA molecular level, are an important platform for study of the assembly and function of viruses. Genome manipulation, such as gene recombination, mosaicism, and mutation may interfere with replication, assembly and release of viruses. An ef...

journal_title：Journal of virological methods

authors： Zheng H,Tian H,Jin Y,Wu J,Shang Y,Yin S,Liu X,Xie Q

更新日期：2009-03-01 00:00:00

abstract：:A near-full genome genotypic assay for HCV1b was developed, which may prove useful to investigate antiviral drug resistance, given new combination therapies for HCV1 infection. The assay consists of three partially overlapping PCRs followed by Sanger population or Illumina next-generation sequencing. Seventy-seven the...

journal_title：Journal of virological methods

authors： Cuypers L,Snoeck J,Vrancken B,Kerremans L,Vuagniaux G,Verbeeck J,Nevens F,Camacho RJ,Vandamme AM,Van Dooren S

更新日期：2014-12-01 00:00:00

abstract：:To investigate various aspects of the latency of pseudorabies virus in swine (PRV, suid herpesvirus 1) we developed in vitro nucleic acid amplification methods based upon the polymerase chain reaction. Primers flanking a 156-bp region of the pseudorabies virus gp II gene were annealed to purified PRV DNA as well as DN...

journal_title：Journal of virological methods

authors： Lokensgard JR,Thawley DG,Molitor TW

更新日期：1991-09-01 00:00:00

abstract：:Plum pox virus (PPV) causes the most destructive viral disease known as plum pox or Sharka disease in stone fruit trees. As an important regulated pathogen, detection of PPV is thus of critical importance to quarantine and eradication of the spreading disease. In this study, the innovative development of two AmplifyRP...

journal_title：Journal of virological methods

authors： Zhang S,Ravelonandro M,Russell P,McOwen N,Briard P,Bohannon S,Vrient A

更新日期：2014-10-01 00:00:00

abstract：:On June 11, 2009, the World Health Organization declared that the influenza A/H1N1/2009 virus had become the first influenza pandemic of the 21st century. Rapid detection and differentiation from seasonal and avian influenza would be beneficial for patient management and infection control. It was the aim of this study...

journal_title：Journal of virological methods

authors： Gunson R,Maclean A,Davies E,Bennett S,Miller R,Carman WF

更新日期：2010-02-01 00:00:00

abstract：:Influenza vaccines must be revised constantly on almost a yearly basis because of the sequential mutations (antigenic drift) that occur as the virus responds to immunologic pressure. New, high yield (hy) reassortant viruses have proved essential to meet production needs for the supply of new vaccines. We have devised ...

journal_title：Journal of virological methods

authors： Brett I,Werber J,Kilbourne ED

更新日期：2002-02-01 00:00:00

abstract：:A technique based on the reverse transcriptase-polymerase chain reaction (RT-PCR) has been developed to detect the presence of Prunus necrotic ringspot virus (PNRSV) and prune dwarf virus (PDV) simultaneously in almond. This paper presents the results of a 3-year study comparing both enzyme-linked immunosorbent assay ...

journal_title：Journal of virological methods

authors： Mekuria G,Ramesh SA,Alberts E,Bertozzi T,Wirthensohn M,Collins G,Sedgley M

更新日期：2003-12-01 00:00:00

abstract：:5-min boiling of 3-fold diluted serum leads to the quantitative release of immune-complexed HIV antigen (Ag) that can be readily measured. The method is capable of detecting as little as 4 to 5 pg/ml Ag p24 depending on the Ag test used. Of a panel of 50 anti-HIV-positive sera, 30 (60%) were Ag-positive after heat den...

journal_title：Journal of virological methods

authors： Schüpbach J,Böni J

更新日期：1993-07-01 00:00:00

abstract：:The first case of pandemic influenza A/H1N1/2009 infection was reported in Mexico in mid-April, 2009, and to date the new H1N1 virus has spread to over 160 countries. Therefore, it is important to obtain reliable epidemiological data on the spread of this virus by novel molecular methods can be developed in detection ...

journal_title：Journal of virological methods

authors： Wu W,Kang X,Bai Z,Liu L,Li J,Wu X,Sun H,Hu T,Yang M,Wang P,Liu L,Yang Y,Di B,Chen W

更新日期：2010-05-01 00:00:00

abstract：:We have developed a murine monoclonal antibody reactive with a major immediate early 72,000 dalton protein of human cytomegalovirus and utilized this reagent in a rapid virus titration and microneutralization assay. Because of the early expression of this virus encoded protein, both assays could be accomplished within...

journal_title：Journal of virological methods

authors： Andreoni M,Faircloth M,Vugler L,Britt WJ

更新日期：1989-02-01 00:00:00

abstract：:Hexon is the major structural protein of adenovirus, and has significance in studies of virus structure and function, vaccine development, and immunodiagnosis. We describe a simple, single-step, anion-exchange high performance liquid chromatography (HPLC) method for the high yield purification of hexon. Purity of the ...

journal_title：Journal of virological methods

authors： Siegel SA,Hutchins JE,Witt DJ

更新日期：1987-09-01 00:00:00

abstract：:Two methods for rapid detection of adenovirus were tested: (i) 24-well plate centrifugation followed by staining with a monoclonal antibody after incubation for 24 h and 48 h, and (ii) pretreatment of A549 cells used in conventional cell culture and 24-well plate centrifugation with 10(-5)M dexamethasone. Twenty-seven...

journal_title：Journal of virological methods

authors： Woods GL,Yamamoto M,Young A

更新日期：1988-06-01 00:00:00

abstract：:A fluorescent DNA probe (DV2.P1) specific to the conserved distal 3'-noncoding region (nucleotides 10653-10678) of dengue 2 virus and a pair of flanking primers (DV2.L1 and DV2.U2) were designed to formulate a dengue 2-specific fluorogenic polymerase chain reaction (PCR). In addition, DV2.L1 was also used as a reverse...

journal_title：Journal of virological methods

authors： Houng HH,Hritz D,Kanesa-thasan N

更新日期：2000-04-01 00:00:00

abstract：:Herpes simplex virus type 1 (HSV-1) UL3 protein is a nuclear protein. In this study, the molecular mechanism of the subcellular localization of UL3 was characterized by fluorescence microscopy in living cells. A nuclear localization signal (NLS) and a nuclear export signal (NES) were also identified. UL3 was demonstra...

journal_title：Journal of virological methods

authors： Zheng C,Lin F,Wang S,Xing J

更新日期：2011-11-01 00:00:00

abstract：:Norovirus is associated commonly with human sewage and is responsible for numerous cases of waterborne and foodborne gastroenteritis every year. Assays using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) have been developed for norovirus, however, accurate detection and quantitation remain pro...

journal_title：Journal of virological methods

authors： Gregory JB,Webster LF,Griffith JF,Stewart JR

更新日期：2011-03-01 00:00:00

abstract：:A technique for coupling foot-and-mouth disease virus (FMDV) to tanned sheep red blood cells (SRBC) is reported. Different parameters influencing the procedure were studied. Subtypes C2, C3, O1 and A24 were used as antigens, and guinea pig hyperimmune sera obtained were tested for specific antibody in passive hemagglu...

journal_title：Journal of virological methods

authors： Pujol CA,Feledi CA,Massouh EJ

更新日期：1988-08-01 00:00:00

abstract：:Silver nanoparticles have demonstrated efficient inhibitory activities against human immunodeficiency virus (HIV) and hepatitis B virus (HBV). However, the effects of silver nanoparticles against H1N1 influenza A virus remain unexplored. In this study, the interaction of silver nanoparticles with H1N1 influenza A viru...

journal_title：Journal of virological methods

authors： Xiang DX,Chen Q,Pang L,Zheng CL

更新日期：2011-12-01 00:00:00

abstract：:In this study, we aim to determine what effects prolonged storage and repeated freeze/thaw cycles have on the stability of influenza A(H1N1)pdm09 (influenza A/H1N1)RNA. Cloned influenza A/H1N1 RNA transcripts were serially diluted from 8.0-1.0 log10 copies/μl. RT-qPCR was used to measure RNA loss in transcripts stored...

journal_title：Journal of virological methods

authors： Granados A,Petrich A,McGeer A,Gubbay JB

更新日期：2017-09-01 00:00:00

abstract：:Portable user-friendly diagnostic tests can benefit detection and surveillance of wildlife diseases. Here, the performance of a compact POCKIT™ Nucleic Acid Analyzer for detection of Ambystoma tigrinum virus (ATV), an emerging Iridovirus that is associated with high host mortality in the western tiger salamander (Amby...

journal_title：Journal of virological methods

authors： Lung O,Reimer SA,Goater CP

更新日期：2017-11-01 00:00:00

abstract：:Bovine herpesvirus 4 (BoHV-4) is a gamma herpesvirus with no clear disease association. Previous studies have demonstrated that macrophages can harbour persistent BoHV-4. Since mesenchymal stem cells in bone marrow regulate the differentiation and proliferation of adjacent haematopoietic precursors, such as macrophage...

journal_title：Journal of virological methods

authors： Donofrio G,Colleoni S,Galli C,Lazzari G,Cavirani S,Flammini CF

更新日期：2005-08-01 00:00:00

abstract：:Ten plasmids containing viral cDNA inserts of portions of the dengue virus type 2 (DEN-2) or Kunjin virus (KUN) genomes were biotinylated using photobiotin acetate and used as probes for the detection of flavivirus RNA in infected Vero cells. The viral cDNA inserts ranged in length from 0.19 to 2.7 kilobase pairs, and...

journal_title：Journal of virological methods

authors： Khan AM,Wright PJ

更新日期：1987-02-01 00:00:00

abstract：:Nipah virus, a zoonotic paramyxovirus which emerged recently was chemically inactivated using binary ethylenimine (BEI). The inactivated virus was concentrated and purified by sucrose gradient centrifugation. The gradient fractions were examined by electron microscopy and Western immunoblot, and gradient fraction cont...

journal_title：Journal of virological methods

authors： Berhane Y,Berry JD,Ranadheera C,Marszal P,Nicolas B,Yuan X,Czub M,Weingartl H

更新日期：2006-03-01 00:00:00

abstract：:The polymerase chain reaction (PCR) and restriction enzyme analysis were used to develop a rapid and simple procedure for identifying geographic subgroups of dengue virus within serotypes for epidemiologic investigations. The entire structural protein region of dengue viruses was amplified and the products were digest...

journal_title：Journal of virological methods

authors： Vorndam V,Kuno G,Rosado N

更新日期：1994-07-01 00:00:00

abstract：:A novel multiplex nested PCR (nPCR) method was developed for detecting and differentiating simultaneously the DNA of polyomaviruses JC, BK and SV40 in a single tube. In the first amplification step the same set of primers were used to amplify a conserved DNA region of the large T antigen gene of JCV, BKV and SV40. The...

journal_title：Journal of virological methods

authors： Fedele CG,Ciardi M,Delia S,Echevarria JM,Tenorio A

更新日期：1999-10-01 00:00:00

abstract：:A method for duplex nested RT-PCR (nRT-PCR) with internal control (IC) for the detection of Nipah virus RNA is described. Incorporation of IC RNA distinguished false and true negative results. The extrinsic RNA was added directly to the PCR master mix and co-amplified with virus specific RNA in a duplex reaction to de...

journal_title：Journal of virological methods

authors： Wacharapluesadee S,Hemachudha T

更新日期：2007-04-01 00:00:00

abstract：:In order to identify new potential antiviral drugs, small amounts of extracts or compounds have to be examined for cytotoxicity and antiviral activity in primary screening using a rapid, easy, inexpensive, and highly standardised test system. In this study, high-throughput cytopathic effect (CPE) inhibitory assays wer...

journal_title：Journal of virological methods

authors： Schmidtke M,Schnittler U,Jahn B,Dahse H,Stelzner A

更新日期：2001-06-01 00:00:00