当前位置： SCI文献检索 > JOURNAL OF VIROLOGICAL METHODS期刊下所有文献 > A quantitative duplex PCR technique for measuring amounts of cell-associated Marek's disease virus: differences in two populations of lymphoma cells.

A quantitative duplex PCR technique for measuring amounts of cell-associated Marek's disease virus: differences in two populations of lymphoma cells.

Abstract：

:A duplex polymerase chain reaction (PCR) was developed to measure Marek's disease virus (MDV) load in two subpopulations of Marek's disease (MD) lymphoma cells from chickens. PCR primers were designed using the sequence of the MDV-ICP4 gene and the chicken IFNgamma gene. Each set of primers was present in the same reaction tube so that the 327 bp ICP4 product and the 420 bp IFNgamma product were co-amplified. Two different fluorescent dyes were used to 5'-end label one PCR primer of each pair to distinguish the IFNgamma and ICP4 products by colour. The IFNgamma PCR product was used as an internal standard enabling comparisons of MDV-ICP4 products between different samples. Neither duplex PCR product was preferentially amplified and both reactions were in their exponential phases when stopped. The products could be distinguished by both size and colour. MD lymphoma cells were taken ex vivo and separated on the basis of expressing a novel host surface antigen recognised by the monoclonal antibody AV37. AV37 + lymphoma cells had greater MDV-loads than AV37 lymphoma cells. The principles used here should be applicable to any cell phenotype and/or cell-associated DNA virus.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Burgess SC,Davison TF

doi

10.1016/s0166-0934(99)00075-0

subject

Has Abstract

pub_date

1999-09-01 00:00:00

pages

27-37

issue

1

eissn

0166-0934

issn

1879-0984

pii

S0166-0934(99)00075-0

journal_volume

82

pub_type

杂志文章

相关文献

JOURNAL OF VIROLOGICAL METHODS文献大全
  • In situ hybridisation and in situ polymerase chain reaction detection of parvovirus B19 DNA within cells.

    abstract：:Modification of an in situ polymerase chain reaction (ISPCR) technique is described for the detection of B19 parvovirus infection. Specific amplification of B19 DNA inside fixed cells was followed by hybridisation with a digoxigenin-labelled probe and then visualised by immunochemical reaction. The assay had higher se...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(94)90164-3

    authors： Gallinella G,Young NS,Brown KE

    更新日期：1994-12-01 00:00:00

  • Development and validation of a foot-and-mouth disease virus SAT serotype-specific 3ABC assay to differentiate infected from vaccinated animals.

    abstract：:The effective control of foot-and-mouth disease (FMD) requires sensitive, specific and rapid diagnostic tools. However, the control and eradication of FMD in Africa is complicated by, among other factors, the existence of five of the seven FMD virus (FMDV) serotypes, including the SAT-serotypes 1, 2 and 3 that are gen...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2018.02.006

    authors： Chitray M,Grazioli S,Willems T,Tshabalala T,De Vleeschauwer A,Esterhuysen JJ,Brocchi E,De Clercq K,Maree FF

    更新日期：2018-05-01 00:00:00

  • Rapid and sensitive detection of hepatitis A virus in representative food matrices.

    abstract：:Hepatitis A virus (HAV) is an important cause of foodborne disease worldwide. The detection of this virus in naturally contaminated food products is complicated by the absence of a reliable culture method, low levels of contamination, and the presence of matrix-associated compounds which inhibit molecular detection. I...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2007.08.024

    authors： Papafragkou E,Plante M,Mattison K,Bidawid S,Karthikeyan K,Farber JM,Jaykus LA

    更新日期：2008-01-01 00:00:00

  • Stability of infectious human coronavirus NL63.

    abstract：:The human coronavirus NL63 was identified in 2004 and subsequent studies showed its worldwide distribution. Infection with this pathogen is associated with upper and lower respiratory tract diseases of mild to moderate severity. Furthermore, HCoV-NL63 is the main cause of croup in children. Within this study an optima...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2014.04.001

    authors： Florek D,Burmistrz M,Potempa J,Pyrc K

    更新日期：2014-09-01 00:00:00

  • The prevalence of porcine teschovirus in the pig population in northeast of China.

    abstract：:The prevalence of porcine teschovirus (PTV) in swine herds in northeast China was investigated. In 2008-2009, 1384 samples of pig sera were collected from 42 farms in Shandong, Hebei, Heilongjiang, and Jilin provinces and in Tianjin City and were tested for specific antibody against PTV-8 by immunofluorescence assay. ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2013.06.005

    authors： Qiu Z,Wang Z,Zhang B,Zhang J,Cui S

    更新日期：2013-10-01 00:00:00

  • Structural and biological properties of Cucumber mosaic virus particles carrying hepatitis C virus-derived epitopes.

    abstract：:The Cucumber mosaic virus (CMV) is a three-component isodiametric plant virus with an extremely wide host range, present worldwide. A pseudorecombinant form has been described, deriving from the RNA3 component of the CMV-S strain, carrying the coat protein (CP) gene, and the RNA 1, 2 components of the CMV-D strain. Th...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2008.10.005

    authors： Nuzzaci M,Bochicchio I,De Stradis A,Vitti A,Natilla A,Piazzolla P,Tamburro AM

    更新日期：2009-02-01 00:00:00

  • Generation and molecular docking analysis of specific single-chain variable fragments selected by phage display against the recombinant nucleocapsid protein of fig mosaic virus.

    abstract：:The mosaic disease caused by fig mosaic virus (FMV) is considered the plague of fig worldwide. A naïve phage display library, raised against the recombinant nucleocapsid protein of FMV (FMV-Np) was screened to obtain specific monoclonal recombinant antibodies in the form of single chain variable fragments (scFvs). Aft...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2019.113796

    authors： Shahmirzaie M,Safarnejad MR,Rakhshandehroo F,Safarpour H,Shirazi FH,Zamanizadeh HR,Elbeaino T

    更新日期：2020-02-01 00:00:00

  • Development and evaluation of a one-step reverse transcription loop-mediated isothermal amplification for detection of Citrus leaf blotch virus.

    abstract：:Citrus leaf blotch virus (CLBV) is a type member of the genus Citrivirus belonging to Betaflexiviridae. In this study, a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method was developed to detect CLBV; this technology has been widely used in the detection of various plant pathogenic microorg...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2019.05.009

    authors： Liu H,Wu W,Tan J,Li Y,Mi W,Jiang L,Wu Y

    更新日期：2019-08-01 00:00:00

  • An evaluation of nine commercial EIA kits for the detection of measles specific IgG.

    abstract：:Nine commercial EIAs for measles-specific IgG were compared with haemagglutination inhibition (HI) and plaque reduction neutralization (PRN). A total of 174 sera selected, to give approximately half of the sera without measles antibody by HI, were tested by all EIAs and HI. However, there was sufficient volume of only...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(97)02210-6

    authors： Hesketh L,Charlett A,Farrington P,Miller E,Forsey T,Morgan-Capner P

    更新日期：1997-06-01 00:00:00

  • Infectious entry of reovirus cores into mammalian cells enhanced by transfection.

    abstract：:The reovirus virion is a moderately complex structure that contains eight structural proteins organized in multiple concentric capsid layers. Mammalian orthoreovirus virions undergo partial uncoating to produce infectious subvirion particles (ISVPs) and cores. Virions and ISVPs are infectious, whereas cores are transc...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2005.04.005

    authors： Jiang J,Coombs KM

    更新日期：2005-09-01 00:00:00

  • Competitive polymerase chain reaction assay for quantitation of HIV-1 DNA and RNA.

    abstract：:A quantitative PCR assay for the detection of HIV-1 nucleic acids is described. The assay is based on a competitive internal standard nucleic acid which can be discriminated from target sequences by the presence of a new restriction enzyme site. The method was used to quantitate plasmid molecules containing HIV-1 sequ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(91)90095-h

    authors： Stieger M,Démollière C,Ahlborn-Laake L,Mous J

    更新日期：1991-09-01 00:00:00

  • Sodium sulphite inhibition of potato and cherry polyphenolics in nucleic acid extraction for virus detection by RT-PCR.

    abstract：:Phenolic compounds from plant tissues inhibit reverse transcription-polymerase chain reaction (RT-PCR). Multiple-step protocols using several additives to inhibit polyphenolic compounds during nucleic acid extraction are common, but time consuming and laborious. The current research highlights that the inclusion of 0....

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(01)00391-3

    authors： Singh RP,Nie X,Singh M,Coffin R,Duplessis P

    更新日期：2002-01-01 00:00:00

  • An assay for quantifying infectious HIV particles.

    abstract：:A method for assessing the number of infectious particles in preparations of HIV has been developed. Virus was mixed with cells to allow binding of virus. The cells were then cast in an agar gel to block any further transfer of virus between the cells. After 4 days of incubation the cells initially infected with HIV e...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(90)90133-z

    authors： Tjøtta E,Hungnes O,Grinde B

    更新日期：1990-02-01 00:00:00

  • Human cytomegalovirus DNA kinetics using a novel HCMV DNA quantitative assay in white blood cells of immunocompromised patients under Ganciclovir therapy.

    abstract：:The clearance of human cytomegalovirus (HCMV) was evaluated in infected patients under Ganciclovir (GCV) treatment, using a novel HCMV DNA quantitation assay (HCMV DNA hybrid capture system, Murex Diagnostics). Peripheral white blood cells (WBC) from whole blood specimens of seven AIDS patients, three kidney and two a...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(97)02180-0

    authors： Payan C,Veal N,Sarol L,Villarmé M,Ngohou C,Riberi P,François S,Ifrah N,Loison J,Chennebault JM,Pichard E,Kouyoumdjian S,Lunel F

    更新日期：1997-04-01 00:00:00

  • Evaluation of an affordable HIV-1 virological failure assay and antiretroviral drug resistance genotyping protocol.

    abstract：:HIV-1 RNA viral load is the preferred tool to monitor virological failure during antiretroviral therapy (ART) exposure. Timely detection of virological failure can reduce the prevalence and complexity of HIV-1 drug resistance. This field evaluation further characterizes a two-step approach to identify virological fail...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2013.08.015

    authors： Bronze M,Aitken SC,Wallis CL,Steegen K,Stuyver LJ,de Wit TF,Stevens W

    更新日期：2013-12-01 00:00:00

  • Development and application of a universal Taqman real-time PCR for quantitation of duck hepatitis B virus DNA.

    abstract：:To develop a quantitative assay for universal detection of duck hepatitis B virus (DHBV) DNA, a Taqman real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) assay was developed using primers and probes based on genomic sequences located at nucleotide 241-414 of the DHBV Core region which possesses the ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2013.03.025

    authors： Wang Y,Li Y,Yang C,Hui L,Han Q,Ma L,Wang Q,Yang G,Liu Z

    更新日期：2013-07-01 00:00:00

  • Simultaneous detection of hemagglutinin and neuraminidase genes of novel influenza A (H7N9) by duplex real-time reverse transcription polymerase chain reaction.

    abstract：:A novel reassortant influenza A (H7N9) virus emerged recently in China. In this study, a duplex real-time reverse transcription polymerase chain reaction (rRT-PCR) assay was developed for the simultaneous detection of hemagglutinin (HA) and neuraminidase (NA) genes of H7N9 influenza viruses. The sensitivity of the ass...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2013.08.021

    authors： Li Y,Wu T,Qi X,Ge Y,Guo X,Wu B,Yu H,Zhu Y,Shi Z,Wang H,Cui L,Zhou M

    更新日期：2013-12-01 00:00:00

  • Production and characterization of monoclonal antibodies against binary ethylenimine inactivated Nipah virus.

    abstract：:Nipah virus, a zoonotic paramyxovirus which emerged recently was chemically inactivated using binary ethylenimine (BEI). The inactivated virus was concentrated and purified by sucrose gradient centrifugation. The gradient fractions were examined by electron microscopy and Western immunoblot, and gradient fraction cont...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2005.09.005

    authors： Berhane Y,Berry JD,Ranadheera C,Marszal P,Nicolas B,Yuan X,Czub M,Weingartl H

    更新日期：2006-03-01 00:00:00

  • Analytical evaluation of the microarray-based FluChip-8G Influenza A+B Assay.

    abstract：BACKGROUND:Influenza causes a significant annual disease burden, with characterization of the infecting virus important in clinical and public health settings. Rapid immunoassays are fast but insensitive, whereas real-time RT-PCR is sensitive but susceptible to genetic mutations and often requires multiple serial assay...

    journal_title：Journal of virological methods

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.jviromet.2019.113686

    authors： Taylor AW,Dawson ED,Blair RH,Johnson JE Jr,Slinskey AH,Smolak AW,Toth E,Liikanen K,Stoughton RS,Smith C,Talbot S,Rowlen KL

    更新日期：2019-11-01 00:00:00

  • Monoclonal antibody against Saint Louis encephalitis prM viral protein.

    abstract：:Saint Louis encephalitis virus belongs to Flavivirus genus; Flaviviridae family jointly with other medically important flaviviruses including dengue virus and West Nile virus. The biological properties and functions of prM flavivirus protein are under investigation due to its importance in the generation of infectious...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2015.01.004

    authors： Pupo-Antúnez M,Vázquez S,Sosa AL,Caballero Y,Vásquez Y,Morier L,Álvarez M,Guzmán MG

    更新日期：2015-06-15 00:00:00

  • Solid-phase enzyme immunoassay for rotavirus antigen: faecal protease activity as a reason for false-negative results.

    abstract：:Rabbits and guinea pigs were immunized with purified bovine rotavirus. Immunoglobulin G fractions of the resulting antisera were used in a standard four-layer solid-phase enzyme immunoassay (EIA) for rotavirus antigen in human faecal specimens. Samples negative for rotavirus in electron microscopy, when diluted in sta...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(82)90096-9

    authors： Hovi T,Väisänen V,Ukkonen P,von Bonsdorff CH

    更新日期：1982-09-01 00:00:00

  • A method for the detection and confirmation of antibodies to hepatitis C virus in dried blood spots.

    abstract：:This study describes the development and evaluation of a cost effective test rationale for the detection of anti-HCV in dried blood spots. Samples were screened using an 'in house' IgG ELISA that incorporated the recombinant proteins c22-3, c200 and NS5. Confirmation of specific antibody to HCV was by a modification o...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(97)00127-4

    authors： Parker SP,Cubitt WD,Ades AE

    更新日期：1997-11-01 00:00:00

  • Simultaneous detection of eight swine reproductive and respiratory pathogens using a novel GeXP analyser-based multiplex PCR assay.

    abstract：:A new high-throughput GenomeLab Gene Expression Profiler (GeXP) analyser-based multiplex PCR assay was developed for the detection of eight reproductive and respiratory pathogens in swine. The reproductive and respiratory pathogens include North American porcine reproductive and respiratory syndrome virus (PRRSV-NA), ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2015.08.001

    authors： Zhang M,Xie Z,Xie L,Deng X,Xie Z,Luo S,Liu J,Pang Y,Khan MI

    更新日期：2015-11-01 00:00:00

  • A non-radioisotopic reverse transcriptase assay using biotin-11-deoxyuridinetriphosphate on primer-immobilized microtiter plates.

    abstract：:We developed a non-radioisotopic (non-RI) reverse transcriptase assay (RTA). The reverse transcriptase (RT) incorporates biotin-11-deoxyuridine-triphosphate (bio-dUTP) using a poly(rA) template hybridized with oligo(dT) primer that is immobilized on the surface of a 96-well microtiter plate. This assay is thus semi-au...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(92)90063-j

    authors： Urabe T,Sano K,Tanno M,Mizoguchi J,Otani M,Lee MH,Takasaki T,Kusakabe H,Imagawa DT,Nakai M

    更新日期：1992-11-01 00:00:00

  • Paramyxovirus infections in ex vivo lung slice cultures of different host species.

    abstract：:Paramyxoviruses, including measles virus (MV), human metapneumovirus (HMPV), human respiratory syncytial virus (HRSV) and canine distemper virus (CDV), are transmitted via the respiratory route. Despite their close phylogenetic relationship, the pathogenesis of these viruses is very different. To study viral tropism a...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2013.06.016

    authors： Nguyen DT,de Vries RD,Ludlow M,van den Hoogen BG,Lemon K,van Amerongen G,Osterhaus AD,de Swart RL,Duprex WP

    更新日期：2013-10-01 00:00:00

  • Determination of hepatitis C virus genotype by Pyrosequencing.

    abstract：:A simple sequencing-based assay is described for genotyping of hepatitis C virus (HCV). RT-PCR was employed to amplify a 237-nucleotide-long fragment from the 5' untranslated region (UTR) of the genome using one biotinylated and one normal primer. Subsequent to capture of the PCR products on streptavidin-coated beads,...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(03)00068-5

    authors： Elahi E,Pourmand N,Chaung R,Rofoogaran A,Boisver J,Samimi-Rad K,Davis RW,Ronaghi M

    更新日期：2003-05-01 00:00:00

  • Use of the polymerase chain reaction for the sensitive detection of St. Louis encephalitis viral RNA.

    abstract：:Polymerase chain reaction (PCR) assays were developed for the detection of RNA from the St. Louis encephalitis (SLE) virus. Using computer-assisted analysis of the MSI-7 strain SLE virus genome, two primer pairs were selected from the capsid-coding and the membrane associated protein-coding genes, and one from the env...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(92)90161-6

    authors： Howe DK,Vodkin MH,Novak RJ,Shope RE,McLaughlin GL

    更新日期：1992-01-01 00:00:00

  • Rapid detection of avian influenza virus H5N1 in chicken tracheal samples using an impedance aptasensor with gold nanoparticles for signal amplification.

    abstract：:Highly pathogenic avian influenza virus H5N1 is a continuous threat to public health and poultry industry. The recurrence of the H5N1 led us to develop a robust, specific, and rapid detection method for the virus. In this study, an impedance aptasensor was developed for the virus detection using specific H5N1 aptamer ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2016.07.018

    authors： Karash S,Wang R,Kelso L,Lu H,Huang TJ,Li Y

    更新日期：2016-10-01 00:00:00

  • A nonisotopic assay method for hepatitis C virus NS5B polymerase.

    abstract：:The RNA-dependent RNA polymerase of hepatitis C virus (HCV) is responsible for replication of genomic RNA. A novel nonisotopic assay method is described for detecting its enzymatic activity. The 5' end of the in vitro-transcribed template RNA was attached covalently to the surface of a Covalink module using carbodiimi...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(01)00443-8

    authors： Park C,Kee Y,Park J,Myung H

    更新日期：2002-03-01 00:00:00

  • Comparison of the Abbott RealTime High Risk HPV test and INNO-LiPA HPV Genotyping Extra test for the detection of human papillomaviruses in formalin-fixed, paraffin-embedded cervical cancer specimens.

    abstract：:Comparative evaluation of Abbott RealTime and Innogenetics INNO-LiPA on alternately processed formalin-fixed, paraffin-embedded specimens of 31 cervical cancers and 31 uterine myomas showed complete agreement in the detection of 14 assay-common HPV genotypes and partial genotyping of HPV-16 and HPV-18. The tissue prep...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2011.04.006

    authors： Kocjan BJ,Seme K,Poljak M

    更新日期：2011-07-01 00:00:00