Abstract：
:Standard methods for assessing the thermal stability of viruses can be time consuming and rather qualitative yet such data is a necessary requisite for vaccine formulation. In this study a novel plate-based thermal scanning assay for virus particle stability has been developed (PaSTRy: Particle Stability Thermal Release Assay). Two environment-sensitive fluorescent dyes, with non-overlapping emission spectra and different affinities, are used to accrue simultaneously independent data for the overall stability of the virus capsid, as judged by the exposure of the genome, and for capsid protein stability according to the exposure of hydrophobic side chains which are normally buried. This offers a fast and efficient high-throughput method to optimise vaccine formulation and to investigate the processes of virus uncoating.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Walter TS,Ren J,Tuthill TJ,Rowlands DJ,Stuart DI,Fry EEdoi
10.1016/j.jviromet.2012.06.014subject
Has Abstractpub_date
2012-10-01 00:00:00pages
166-70issue
1eissn
0166-0934issn
1879-0984pii
S0166-0934(12)00223-6journal_volume
185pub_type
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