Use of the immuno-gold technique for in situ localization of cauliflower mosaic virus (CaMV) particles and the major protein of the inclusion bodies.

abstract：:Introduction of amino acids substitutions in murine leukemia virus genome is a powerful method to determine the relative importance of various viral factors in pathogenesis. However, introduction of such amino acids substitution could result in viruses at a selective disadvantage, and eventual selection of revertants....

journal_title：Journal of virological methods

authors： Danis C,Rassart E,Lemay G

更新日期：2005-05-01 00:00:00

abstract：:Cervical carcinoma is one of the major consequences of human papillomavirus (HPV) infections. Although HPV infections of cervix do not always progress to cancer, 90% cases of cervical cancer have been found associated with high risk HPV (hrHPV) infection. Usually, HPV infection is asymptomatic; however, this asymptoma...

journal_title：Journal of virological methods

authors： Ramzan M,Noor ul Ain,Ilyas S,Umer M,Bano S,Sarwar S,Shahzad N,Shakoori AR

更新日期：2015-09-15 00:00:00

abstract：:Previous work demonstrated recently the adaptation of the Escherichia coli biotin ligase BirA - biotin acceptor sequence (BAS) labeling system to produce human immunodeficiency virus type 1 viruses with biotinylated integrase (NLXIN(B)) and matrix (NLXMA(B)) proteins (Belshan et al., 2009). This report describes the c...

journal_title：Journal of virological methods

authors： Belshan M,Matthews JM,Madson CJ

更新日期：2011-01-01 00:00:00

abstract：:An H5N1 avian influenza virus (AIV) hemagglutinin (HA) protein pseudotyped lentivirus, HIV/H5-HA, was generated, characterized in vitro and evaluated for its ability to induce protective immunity against virulent wild type AIV in mice. The HIV/H5-HA virus was able to infect 293T, BHK, Vero, PK-15, MDCK cells but not I...

journal_title：Journal of virological methods

authors： Zhang S,Xiao L,Zhou H,Yu Z,Chen H,Guo A,Jin M

更新日期：2008-12-01 00:00:00

abstract：:TaqMan and SYBR Green quantitative PCR (qPCR) methods were developed as DNA-based approaches to reproducibly enumerate M13 and T7 phages from phage display selection experiments individually and simultaneously. The genome copies of M13 and T7 phages were quantified by TaqMan or SYBR Green qPCR referenced against M13 a...

journal_title：Journal of virological methods

authors： Peng X,Nguyen A,Ghosh D

更新日期：2018-02-01 00:00:00

abstract：:Immunofluorescence assays (IFA) that simultaneously distinguish between antibodies against closely related human immunodeficiency virus type 1 (HIV-1) and type 2 (HIV-2) infections have not been readily available. Serum specimens from 95 HIV-1-infected, 26 HIV-2-infected and 3 HIV-1/HIV-2 dually infected individuals a...

journal_title：Journal of virological methods

authors： Tanabe-Tochikura A,Ang Singh MT,Tsuchie H,Zhang J,Paladin FJ,Kurimura T

更新日期：1995-04-01 00:00:00

abstract：:Hepatitis D virus (HDV) is a defective virus which requires hepatitis B virus (HBV) surface antigen (HBsAg) for its assembly. Hepatitis B infected individuals co-infected or superinfected with HDV often present with more severe hepatitis, progress faster to liver disease, and have a higher mortality rate than individu...

journal_title：Journal of virological methods

authors： Kodani M,Martin A,Mixson-Hayden T,Drobeniuc J,Gish RR,Kamili S

更新日期：2013-11-01 00:00:00

abstract：:Certification represents the first line of defense against fruit tree viruses. For certification or surveys dealing with large number of samples, ELISA is still considered the technique of choice and requires a continuous supply of good quality antibodies. Prune dwarf virus (PDV) is among the major viruses affecting s...

journal_title：Journal of virological methods

authors： Abou-Jawdah Y,Sobh H,Cordahi N,Kawtharani H,Nemer G,Maxwell DP,Nakhla MK

更新日期：2004-10-01 00:00:00

abstract：:The disruption of the viral coat of human immunodeficiency virus by Triton X-100, a nonionic detergent, is a time-dependent process which requires incubation times of 30 min or longer. Conditions for the production of a noninfectious sample from a viral pellet that can be used to measure reverse transcriptase activity...

journal_title：Journal of virological methods

authors： White EL,Bowdon BJ,Shannon WM

更新日期：1998-01-01 00:00:00

abstract：:A simple and rapid solid-phase reverse transcriptase assay was developed based on the use of poly(rA):oligo(dT)12-18 as template primer immobilized on DEAE cellulose paper squares to detect human immunodeficiency virus (HIV) and/or other retroviruses in cell culture supernatants. It was found that PEG (per se) -up to ...

journal_title：Journal of virological methods

authors： Somogyi PA,Gyuris A,Földes I

更新日期：1990-03-01 00:00:00

abstract：:Standard methods for assessing the thermal stability of viruses can be time consuming and rather qualitative yet such data is a necessary requisite for vaccine formulation. In this study a novel plate-based thermal scanning assay for virus particle stability has been developed (PaSTRy: Particle Stability Thermal Relea...

journal_title：Journal of virological methods

authors： Walter TS,Ren J,Tuthill TJ,Rowlands DJ,Stuart DI,Fry EE

更新日期：2012-10-01 00:00:00

abstract：:Simian betaretroviruses (formerly Type D retroviruses; SRV) are a group of closely related retroviruses for which the natural host species are Asian monkeys of the genus Macaca. Five serotypes have been identified by classical neutralization assays and three additional untyped variants have been reported (SRV(Tsukuba)...

journal_title：Journal of virological methods

authors： White JA,Todd PA,Rosenthal AN,Yee JL,Grant R,Lerche NW

更新日期：2009-12-01 00:00:00

abstract：:Herpes simplex virus type 1 (HSV-1) amplicon preparations are usually quantified as transducing units/ml (TU/ml), with little information on genomic copy/TU ratios. In the present study, two HSV-1 amplicons expressing enhanced green fluorescent protein (EGFP) were analysed by quantitative PCR (qPCR) and transducing ac...

journal_title：Journal of virological methods

authors： El-Sherbini YM,Stevenson MM,Seymour LW,Wade-Martins R

更新日期：2009-08-01 00:00:00

abstract：:A fluorogenic reverse transcriptase-polymerase chain reaction (RT-PCR) system was developed for use as a rapid diagnostic test for determining dengue viremia. The dengue virus 3'-noncoding sequence was utilized to formulate serotype-specific RT-PCR assays for quantitative identification of the four different dengue vi...

journal_title：Journal of virological methods

authors： Houng HS,Chung-Ming Chen R,Vaughn DW,Kanesa-thasan N

更新日期：2001-06-01 00:00:00

abstract：:The appearance of cytomegalovirus (CMV) proteins in infected fibroblasts was determined by flow cytometry. The sequential production of immediate early (IE), early (E), and late (L) proteins reacting with respective monoclonal antibodies (mAbs) E13, 58/5, and 24/4 was determined in fibroblasts infected with the AD-169...

journal_title：Journal of virological methods

authors： Torigoe S,Campbell DE,Torigoe F,Michelson S,Starr SE

更新日期：1993-12-15 00:00:00

abstract：:A multiplex polymerase chain reaction (mPCR) assay was developed to detect six RNA and one DNA citrus virus: Citrus leaf rugose virus (CLRV), Citrus psorosis virus (CPsV), Citrus tatter leaf virus (CTLV), Citrus tristeza virus (CTV), Citrus variegation virus (CVV), Citrus yellow mosaic virus (CYMV), and Indian citrus ...

journal_title：Journal of virological methods

authors： Roy A,Fayad A,Barthe G,Brlansky RH

更新日期：2005-10-01 00:00:00

abstract：:A competitive reverse transcription-polymerase chain reaction (RT-PCR) was developed to detect and to quantitate the RNA of group A rotaviruses. In the assay, a 433 bp fragment is amplified by a one-tube RT-PCR protocol using primers with binding sites located in a highly conserved region of segment 6 of the rotavirus...

journal_title：Journal of virological methods

authors： Schwarz BA,Bange R,Vahlenkamp TW,Johne R,Müller H

更新日期：2002-09-01 00:00:00

abstract：:Most transplant centers screen kidney transplant recipients for BK virus (BKV) infection using molecular techniques for the virus load determination. However, there is no consensus about the pre-analytical methods involved in the viral detection. In this study BK viral load was compared by the means of two urine treat...

journal_title：Journal of virological methods

authors： Pinto GG,Poloni JA,Carneiro LC,Baethgen LF,Barth AL,Pasqualotto AC

更新日期：2013-03-01 00:00:00

abstract：:In March/April 2009, Mexico experienced an outbreak of respiratory illness, due to a new influenza of swine origin virus, which spread rapidly via human-to-human transmission, and became pandemic (A/H1N1pdm). Because of its unique genome composition, which includes gene segments of swine, avian and human origin, and t...

journal_title：Journal of virological methods

authors： Lalle E,Bordi L,Castilletti C,Meschi S,Selleri M,Carletti F,Lapa D,Travaglini D,Ippolito G,Capobianchi MR,Di Caro A

更新日期：2010-02-01 00:00:00

abstract：:Reverse genetics systems, with the ability to manipulate viral genomes at the DNA molecular level, are an important platform for study of the assembly and function of viruses. Genome manipulation, such as gene recombination, mosaicism, and mutation may interfere with replication, assembly and release of viruses. An ef...

journal_title：Journal of virological methods

authors： Zheng H,Tian H,Jin Y,Wu J,Shang Y,Yin S,Liu X,Xie Q

更新日期：2009-03-01 00:00:00

abstract：:As a prelude to the development of nucleic acid probes specific for non-A, non-B hepatitis virus(es) (NANBV), plasmas with alanine aminotransferase levels greater than or equal to 110 IU were assayed for DNA by a radioimmunoassay. Approximately 50% of such plasmas are expected to contain NANBV. One-hundred and seventy...

journal_title：Journal of virological methods

authors： Neurath AR,Strick N,Miller K,Waldman AA

更新日期：1984-02-01 00:00:00

abstract：:In preparation for field trials, a nuclear polyhedrosis virus (NPV) of the pine beauty moth, Panolis flammea, was mass produced in vivo in an alternative (heterologous) host, the cabbage moth, Mamestra brassicae. Simultaneously, Mamestra NPV was also produced in M. brassicae. This homologous NPV/host system was a cons...

journal_title：Journal of virological methods

authors： Kelly PM,Entwistle PF

更新日期：1988-03-01 00:00:00

abstract：:Highly pathogenic avian influenza virus H5N1 is a continuous threat to public health and poultry industry. The recurrence of the H5N1 led us to develop a robust, specific, and rapid detection method for the virus. In this study, an impedance aptasensor was developed for the virus detection using specific H5N1 aptamer ...

journal_title：Journal of virological methods

authors： Karash S,Wang R,Kelso L,Lu H,Huang TJ,Li Y

更新日期：2016-10-01 00:00:00

abstract：:The study of replication of viruses that require high bio-secure facilities can be accomplished with less stringent containment using non-infectious 'replicon' systems. The FMDV replicon system (pT7rep) reported by Mclnerney et al. (2000) was modified by the replacement of sequences encoding chloramphenicol acetyl-tra...

journal_title：Journal of virological methods

authors： Tulloch F,Pathania U,Luke GA,Nicholson J,Stonehouse NJ,Rowlands DJ,Jackson T,Tuthill T,Haas J,Lamond AI,Ryan MD

更新日期：2014-12-01 00:00:00

abstract：:The World Health Organization recommends screening donor blood for HIV in centralized laboratories. This recommendation contributes to quality, but presents specimen transport challenges for resource-limited settings which may be relieved by using dried blood spots (DBS). In sub-Saharan Africa, most countries screen d...

journal_title：Journal of virological methods

authors： Okonji JA,Basavaraju SV,Mwangi J,Shiraishi RW,Odera M,Ouma K,Pitman JP,Marum LH,Ou CY,Zeh C

更新日期：2012-01-01 00:00:00

abstract：:Human enteroviruses and rhinoviruses are respiratory pathogens whose role in acute respiratory infection is underestimated due to the use of diagnostic procedures with poor sensitivity. To determine the prevalence of these two pathogens in the upper respiratory tract infections, a multiplex procedure was developed tha...

journal_title：Journal of virological methods

authors： Billaud G,Peny S,Legay V,Lina B,Valette M

更新日期：2003-03-01 00:00:00

abstract：:Acute bee paralysis virus (ABPV), Black queen cell virus (BQCV), Chronic bee paralysis virus (CBPV), Deformed wing virus (DWV), Sacbrood virus (SBV) and Varroa destructor virus 1 (VDV1) are the six main honeybee viruses reported in Europe. We assessed the accuracy (trueness and precision) of reverse transcriptase quan...

journal_title：Journal of virological methods

authors： Schurr F,Tison A,Militano L,Cheviron N,Sircoulomb F,Rivière MP,Ribière-Chabert M,Thiéry R,Dubois E

更新日期：2019-08-01 00:00:00

abstract：:A method for duplex nested RT-PCR (nRT-PCR) with internal control (IC) for the detection of Nipah virus RNA is described. Incorporation of IC RNA distinguished false and true negative results. The extrinsic RNA was added directly to the PCR master mix and co-amplified with virus specific RNA in a duplex reaction to de...

journal_title：Journal of virological methods

authors： Wacharapluesadee S,Hemachudha T

更新日期：2007-04-01 00:00:00

abstract：:The in vitro syncytium induction capacity of human immunodeficiency virus type 1 (HIV1) isolates is an important marker in the progression of the disease. Two methods have been widely used to determine the biological phenotype of HIV1. These two methods, the direct MT-2 assay and the supernatant assay, were compared f...

journal_title：Journal of virological methods

authors： Liesnard C,Delforge ML,Tchetcheroff M,De Maertelaer V,Farber CM,Van Vooren JP

更新日期：1997-03-01 00:00:00

abstract：:Bluetongue (BT) is an arboviral disease, which can often be fatal in naïve sheep and white tailed deer, but is usually less severe, or unapparent in other ruminants. Twenty-six bluetongue virus (BTV) serotypes have been recognised so far, two of which (BTV-25 and BTV-26) were recently identified by phylogenetic compar...

journal_title：Journal of virological methods

authors： Maan NS,Maan S,Belaganahalli M,Pullinger G,Montes AJ,Gasparini MR,Guimera M,Nomikou K,Mertens PP

更新日期：2015-03-01 00:00:00