Sensitive analytic ELISAs for subclass herpes virus IgG.

abstract：:A simple method for evaluating the virucidal activity of water-soluble antiseptic and disinfectant products using poliovirus type 1 (Sabin strain) is described. Using a commercial concentrator, kinetic studies of four products were carried out. It was shown that 2% glutaraldehyde and 5% povidone iodine are rapidly vir...

journal_title：Journal of virological methods

authors： Boudouma M,Enjalbert L,Didier J

更新日期：1984-12-01 00:00:00

abstract：:Polymerase chain reaction (PCR) assays were developed for the detection of RNA from the St. Louis encephalitis (SLE) virus. Using computer-assisted analysis of the MSI-7 strain SLE virus genome, two primer pairs were selected from the capsid-coding and the membrane associated protein-coding genes, and one from the env...

journal_title：Journal of virological methods

authors： Howe DK,Vodkin MH,Novak RJ,Shope RE,McLaughlin GL

更新日期：1992-01-01 00:00:00

abstract：:The amplification of the 468 bp fragment of the BHV-1 genome by PCR is described. The 22 bp oligomers from the BHV-1 gI gene were used as primers. For successful amplification the thermal denaturation (100 degrees C/8 min, ice) of the DNA sample was carried out prior to the cycling (95 degrees C for 1 min, 56 degrees ...

journal_title：Journal of virological methods

authors： Vilcek S

更新日期：1993-02-01 00:00:00

abstract：:A rapid and efficient method is described for isotype quantitation of antiviral antibodies in mice infected with Theiler's murine encephalomyelitis virus (TMEV). Serum antibodies were reacted with fluorochrome-labeled TMEV in a modified fluid-phase particle concentration fluorescence immunoassay (PCFIA). Biotin and av...

journal_title：Journal of virological methods

authors： Peterson JD,Miller SD,Waltenbaugh C

更新日期：1990-02-01 00:00:00

abstract：:An international collaborative study was performed to evaluate a set of PCR reference reagents for HIV diagnosis. Twenty-six laboratories from 9 countries analysed a proficiency panel of 10 coded DNA samples using the PCR reference reagents and protocols. For comparison, these coded samples were then assessed using a ...

journal_title：Journal of virological methods

authors： Bootman JS,Kitchin PA

更新日期：1992-04-01 00:00:00

abstract：:An alternative enzyme linked immunosorbent assay (ELISA) system was developed to analyze antibodies to human papillomavirus capsid antigens. The assay uses glutathione crosslinked to casein to capture the major capsid protein L1 from human papillomavirus (HPV) types 6b, 16 and 18 fused to glutathione S-transferase (GS...

journal_title：Journal of virological methods

authors： Sehr P,Müller M,Höpfl R,Widschwendter A,Pawlita M

更新日期：2002-10-01 00:00:00

abstract：:Reverse transcription loop-mediated isothermal amplification (RT-LAMP) was applied to the detection of equine influenza virus (EIV). Because equine influenza is caused currently by EIV of the H3H8 subtype, the RT-LAMP primer set was designed to target the hemagglutinin gene of this subtype. The detection limit of the ...

journal_title：Journal of virological methods

authors： Nemoto M,Yamanaka T,Bannai H,Tsujimura K,Kondo T,Matsumura T

更新日期：2011-12-01 00:00:00

abstract：:Portable user-friendly diagnostic tests can benefit detection and surveillance of wildlife diseases. Here, the performance of a compact POCKIT™ Nucleic Acid Analyzer for detection of Ambystoma tigrinum virus (ATV), an emerging Iridovirus that is associated with high host mortality in the western tiger salamander (Amby...

journal_title：Journal of virological methods

authors： Lung O,Reimer SA,Goater CP

更新日期：2017-11-01 00:00:00

abstract：:The development of a rapid and sensitive assay for detection of replication-competent adenoviruses (RCAs) is described. This RCA assay consists of an incubation step of 4 days of adenoviral vectors on A549 cells in a microcarrier cell culture system followed by detection of amplified RCAs by E1-specific quantitative P...

journal_title：Journal of virological methods

authors： Schalk JA,de Vries CG,Orzechowski TJ,Rots MG

更新日期：2007-11-01 00:00:00

abstract：:Single-stranded conformation polymorphism (SSCP) is a technique used widely for the detection of differences in DNA sequence based on PCR technology. Developed by geneticists for the detection of mutations causing disease, it has been adopted more recently for the analysis of the quasi-species of viral genomes, such a...

journal_title：Journal of virological methods

authors： McKechnie VM,McCruden EA

更新日期：2001-04-01 00:00:00

abstract：:A technique for coupling foot-and-mouth disease virus (FMDV) to tanned sheep red blood cells (SRBC) is reported. Different parameters influencing the procedure were studied. Subtypes C2, C3, O1 and A24 were used as antigens, and guinea pig hyperimmune sera obtained were tested for specific antibody in passive hemagglu...

journal_title：Journal of virological methods

authors： Pujol CA,Feledi CA,Massouh EJ

更新日期：1988-08-01 00:00:00

abstract：:The availability of whole genome sequencing has contributed to many aspects of dengue research, and its use in dengue virus (DENV) surveillance for early epidemic warning has been proposed. Methods to sequence the genomes of individual dengue serotypes have been described previously, but no single method is known to b...

journal_title：Journal of virological methods

authors： Christenbury JG,Aw PP,Ong SH,Schreiber MJ,Chow A,Gubler DJ,Vasudevan SG,Ooi EE,Hibberd ML

更新日期：2010-10-01 00:00:00

abstract：:Lactate dehydrogenase-elevating virus (LDV) can infect transplantable mouse tumors or xenograft tumors in mice through LDV-contaminated mouse biological materials, such as Matrigel, or through mice infected with LDV. LDV infects specifically mouse macrophages and alters immune system and tumor phenotype. The tradition...

journal_title：Journal of virological methods

authors： Liu H,Bockhorn J,Dalton R,Chang YF,Qian D,Zitzow LA,Clarke MF,Greene GL

更新日期：2011-05-01 00:00:00

abstract：:A simple immunoperoxidase assay (IPA), adapted for detection of serum IgM antibodies to cytomegalovirus (CMV) is described. The antigen consisted of CMV infected human embryonic fibroblasts or isolated nuclei. The sera were absorbed with aggregated gamma-globulins prior to testing. Rabbit anti-human IgM peroxidase con...

journal_title：Journal of virological methods

authors： Singer Y,Kimmel N,Sarov I

更新日期：1985-05-01 00:00:00

abstract：:Viral susceptibility testing has been traditionally performed by the plaque reduction assay (PRA) which is laborious, time consuming, relatively expensive, and requires subjective input by the reader. An in situ cellular enzyme-linked immunosorbent assay (ELISA) has been developed with the potential to overcome many o...

journal_title：Journal of virological methods

authors： Myc A,Anderson MJ,Baker JR Jr

更新日期：1999-02-01 00:00:00

abstract：:The production of monoclonal antibodies (mAbs) to a common antigenic region on rabbit haemorrhagic disease virus (RHDV) has enabled the development of a capture ELISA for virus detection. The assay was shown to detect reliably the presence of viral antigen in crude homogenates of a range of rabbit tissues and has prov...

journal_title：Journal of virological methods

authors： Collins BJ,White JR,Lenghaus C,Morrissy CJ,Westbury HA

更新日期：1996-04-26 00:00:00

abstract：:Hepatitis E virus (HEV) is the causative agent of hepatitis E, a food- and water-borne disease. In developed countries, consumption of meats from pigs, wild boars and deer is a major source of infection. Although HEV and HEV-related viruses have been detected in many animal species, their zoonotic potential and preval...

journal_title：Journal of virological methods

authors： Yonemitsu K,Terada Y,Kuwata R,Nguyen D,Shiranaga N,Tono S,Matsukane T,Yokoyama M,Suzuki K,Shimoda H,Takano A,Muto M,Maeda K

更新日期：2016-12-01 00:00:00

abstract：:Certification represents the first line of defense against fruit tree viruses. For certification or surveys dealing with large number of samples, ELISA is still considered the technique of choice and requires a continuous supply of good quality antibodies. Prune dwarf virus (PDV) is among the major viruses affecting s...

journal_title：Journal of virological methods

authors： Abou-Jawdah Y,Sobh H,Cordahi N,Kawtharani H,Nemer G,Maxwell DP,Nakhla MK

更新日期：2004-10-01 00:00:00

abstract：:The consumption of bivalve shellfish is a common cause of foodborne outbreaks of viral origin. The evaluation of the sanitary quality of these products, however, is still based on bacterial indicators of fecal contamination (Reg. (EC) No. 2073/2005 and No.1441/2007) even if it is known that they are not reliable indic...

journal_title：Journal of virological methods

authors： Di Pasquale S,Paniconi M,De Medici D,Suffredini E,Croci L

更新日期：2010-01-01 00:00:00

abstract：:Diagnostic tests are usually evaluated in terms of simple qualitative measures of sensitivity and specificity. When comparing different quantitative assays such as ELISAs, it is often more useful to deal with actual values (sample optical density/cut-off optical density ratio (OD ratio] rather than the qualitative rel...

journal_title：Journal of virological methods

authors： Crofts N,Maskill W,Gust ID

更新日期：1988-10-01 00:00:00

abstract：:Antigenaemia due to human immunodeficiency virus (HIV) is thought to be significant either before the appearance of a specific antibody response, or after its decline during terminal stages. In order to increase the rate of detection of HIV antigen carriers, regardless of the stage or despite the presence of specific ...

journal_title：Journal of virological methods

authors： Ruiz-Arguelles A,Ponce-de-Leon S,Presno-Bernal M,Ponce-de-Leon A,Ortiz-Lopez R,Rojas G

更新日期：1988-12-01 00:00:00

abstract：:Rapid detection of novel swine origin influenza A virus (S-OIV) (H1N1) is crucial for timely implementation of infection control measures. In this study, a haemagglutinin (HA) gene-based real-time nucleic acid sequence-based amplification (NASBA) assay was developed for the specific detection of S-OIV (H1N1). The assa...

journal_title：Journal of virological methods

authors： Ge Y,Cui L,Qi X,Shan J,Shan Y,Qi Y,Wu B,Wang H,Shi Z

更新日期：2010-02-01 00:00:00

abstract：:The inactivation of hepatitis B virus (HBV) using two commercially available disinfectants was analysed. Indirect evidence of virus inactivation was obtained by examining the decrease in HBV-associated DNA polymerase and HBcAg activities after treatment with increasing concentrations of disinfectant. Inactivation was ...

journal_title：Journal of virological methods

authors： Howard CR,Dixon J,Young P,van Eerd P,Schellekens H

更新日期：1983-09-01 00:00:00

abstract：:A quantitative PCR method was developed in order to quantitate the number of copies of Pseudorabies virus (PRV) genome present in tissues from infected pigs. The method is based on the use of an internal standard that differs from the target DNA by a deletion of ten base pairs, and that is co-amplified with the target...

journal_title：Journal of virological methods

authors： Thiery R,Pannetier C,Rziha HJ,Jestin A

更新日期：1996-09-01 00:00:00

abstract：:Rapid alternative methods are required to evaluate easily acyclovir (ACV) sensitivity of clinical herpes simplex virus (HSV) isolates. The objective of this study was to screen 54 ACV-sensitive and 41 ACV-resistant clinical HSV-1 isolates, well characterized by phenotypic and genotypic methods, for the phosphorylation...

journal_title：Journal of virological methods

authors： Sauerbrei A,Vödisch S,Bohn K,Schacke M,Gronowitz S

更新日期：2013-03-01 00:00:00

abstract：:A combined reverse-transcription polymerase chain reaction (RT-PCR) method was developed for the detection and differentiation of wild-type and vaccine strains of the canine distemper virus (CDV). A pair of primers (P1/P2) was used to detect both CDV wild-type strains and vaccines. Another pair (P3/P4) was used to det...

journal_title：Journal of virological methods

authors： Yi L,Cheng S,Xu H,Wang J,Cheng Y,Yang S,Luo B

更新日期：2012-01-01 00:00:00

abstract：:The species cowpox virus (CPXV), genus Orthopoxvirus (OPV), consists of isolates highly variable in their biological properties and their genotypes. A TaqMan PCR assay for the specific detection of CPXV DNA based on sequences of the ORF D11L has been developed recently. (Gavrilova et al., 2010; Shchelkunov et al., 201...

journal_title：Journal of virological methods

authors： Maksyutov RA,Gavrilova EV,Meyer H,Shchelkunov SN

更新日期：2015-01-01 00:00:00

abstract：:A loop-mediated isothermal amplification (LAMP) assay was developed for the detection of abalone herpesvirus DNA. Two pairs of primers were designed, based on the sequence of the DNA polymerase gene of abalone herpesvirus. The reaction temperature and time were optimized to 63°C and 60min, respectively. LAMP amplicons...

journal_title：Journal of virological methods

authors： Chen MH,Kuo ST,Renault T,Chang PH

更新日期：2014-02-01 00:00:00

abstract：:To develop reverse genetics system of RNA viruses, cloning of full-length viral genome is required which is often challenging due to many steps involved. In this study, we report cloning of full-length cDNA from an Indian field isolate (CSFV/IVRI/VB-131) of classical swine fever virus (CSFV) using in vitro overlap ext...

journal_title：Journal of virological methods

authors： Kamboj A,Saini M,Rajan LS,Patel CL,Chaturvedi VK,Gupta PK

更新日期：2015-12-15 00:00:00

abstract：:Human papillomavirus (HPV) infection is the primary cause of cervical cancer. The Quantivirus(®) HPV E6/E7 RNA 3.0 assay (DiaCarta, CA, USA) detects E6/E7 mRNA of 13 high risk subtypes and 6 low risk subtypes. Cervical specimens collected in PreservCyt were processed for HPV detection. Cervical biopsies were taken onl...

journal_title：Journal of virological methods

authors： Shen Y,Gong J,He Y,Cheng G,Okunieff P,Li X

更新日期：2013-02-01 00:00:00