Abstract：
:Cell membranes were prepared from herpes simplex virus (HSV) type 1-infected cells and solubilized with a low concentration of sodium deoxycholate. The supernatant after ultracentrifugation was used as antigen in a newly developed solid phase assay, diffusion in gel-enzyme-linked immunosorbent assay (DIG-ELISA). Antigen solubilization was almost complete, all HSV glycoproteins were represented, the yield of antigen in the solubilization process was high, and the presence of a detergent, sodium deoxycholate, did not interfere with the adsorption of antigen to the solid phase. DIG-ELISA was compared with the neutralization test for the determination of HSC antibodies and zone diameters showed a good correlation with the titre obtained by the neutralization test.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Jeansson S,Elwing H,Nygren H,Olofsson Sdoi
10.1016/0166-0934(82)90045-3subject
Has Abstractpub_date
1982-04-01 00:00:00pages
167-76issue
3eissn
0166-0934issn
1879-0984pii
0166-0934(82)90045-3journal_volume
4pub_type
杂志文章abstract：:A method for co-amplification of multiple viral sequences of HIV-1 and HIV-2 by polymerase chain reaction was designed. The technique resulted in the specific detection of each type of virus and allowed the amplification of as few as two copies of target DNA. The amplification of multiple regions of the viral genome o...
journal_title：Journal of virological methods
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doi：10.1016/0166-0934(94)90053-1
更新日期：1994-08-01 00:00:00
abstract：:Sensitive, precise and rapid detection tests are needed in the quality control of rabies vaccine for rabies virus nucleoprotein. Previous studies for quantitation of rabies virus nucleoprotein focused on enzyme-linked immunosorbent assay (ELISA). A novel immunoassay for rapid determination of rabies virus nucleoprotei...
journal_title：Journal of virological methods
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abstract：:The polymerase chain reaction (PCR) methods enable the detection of large number of human papillomavirus (HPV) genotypes that infect the anogenital tract. In this study, two groups of cervical scrapes with abnormal cytomorphology were analysed. The first group was tested with three sets of consensus primers located wi...
journal_title：Journal of virological methods
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doi：10.1016/s0166-0934(00)00194-4
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journal_title：Journal of virological methods
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abstract：:The simple spot hybridization technique to detect HBV DNA in serum was modified to concentrate Dane particles by pelleting. This minimised interference by serum components and allowed larger volumes of sera (up to 500 microliters) to be used in serial dilution on borderline positive samples and increased the efficienc...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(85)90136-3
更新日期：1985-12-01 00:00:00
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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doi：10.1016/j.jviromet.2006.11.010
更新日期：2007-03-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
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更新日期：1992-08-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(94)90043-4
更新日期：1994-09-01 00:00:00
abstract：:Avian influenza viruses (AIVs) of the H9N2 subtype are a major economic problem in the poultry industry in Israel. Most field isolates from the last decade differ significantly from H9N2 isolates from Europe and the USA, rendering published detection methods inadequate. This study aimed to develop a real-time TaqMan((...
journal_title：Journal of virological methods
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2008.03.023
更新日期：2008-07-01 00:00:00
abstract：:A one-tube real-time TaqMan reverse transcription-polymerase chain reaction (RT-PCR) assay was developed for the detection of equine arteritis virus (EAV). The test was validated using the seminal plasma and nasal secretions of infected horses that were proven to contain EAV by traditional virus isolation in rabbit ki...
journal_title：Journal of virological methods
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(84)90042-9
更新日期：1984-02-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2021.114062
更新日期：2021-01-08 00:00:00
abstract：:A rapid rate-kinetic turbidometric assay for the quantitation of viral complement fixing antibodies has been developed, using adenovirus as a model. The procedure is based on the turbidometric quantitation of intact sheep erythrocytes and measures the rate of hemolysis (change in absorbance at 640 nm/min/), at maximum...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(85)90003-5
更新日期：1985-10-01 00:00:00
abstract：:A sensitive and specific blocking enzyme-linked immunosorbent assay (ELISA) was developed to distinguish infectious bovine rhinotracheitis virus (IBRV)-infected animals from those immunized with a glycoprotein gIII deletion mutant, IBRV(NG)dltkdlgIII. For this ELISA, undiluted test sera are used to block the binding o...
journal_title：Journal of virological methods
pub_type： 杂志文章
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abstract：BACKGROUND:Molecular assays for diagnosis of Flu A, Flu B, and RSV with short turn-around-time (TAT) are of considerable clinical importance. In addition, rapid and accurate diagnosis of a large panel of viral and atypical pathogens can be crucial in immunocompromised patients. OBJECTIVES:First, to evaluate the perfor...
journal_title：Journal of virological methods
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doi：10.1016/j.jviromet.2017.03.013
更新日期：2017-07-01 00:00:00
abstract：:Transmission electron microscopy can be used to observe the ultrastructure of viruses and other microbial pathogens with nanometer resolution. In a transmission electron microscope (TEM), the image is created by passing an electron beam through a specimen with contrast generated by electron scattering from dense eleme...
journal_title：Journal of virological methods
pub_type： 杂志文章
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2005.05.008
更新日期：2005-10-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2005.01.010
更新日期：2005-05-01 00:00:00
abstract：:Gene transfer into hepatocytes is highly desirable for the long-term goal of replacing deficient proteins and correcting metabolic disorders. Vectors based on herpes simplex virus type-1 (HSV-1) have been demonstrated to mediate efficient gene transfer into hepatocytes both in vitro and in vivo. Large transgene capaci...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2004.09.007
更新日期：2005-01-01 00:00:00
abstract：:A rapid coliphage detection assay was developed, based on the phage-induced release of beta-galactosidase from cells of Escherichia coli. The assay could detect as few as five coliphage per sample without an overnight incubation period. The range of acceptable assay parameters was identified. ...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/s0166-0934(00)00253-6
更新日期：2001-01-01 00:00:00
abstract：:Previous work demonstrated recently the adaptation of the Escherichia coli biotin ligase BirA - biotin acceptor sequence (BAS) labeling system to produce human immunodeficiency virus type 1 viruses with biotinylated integrase (NLXIN(B)) and matrix (NLXMA(B)) proteins (Belshan et al., 2009). This report describes the c...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2010.11.005
更新日期：2011-01-01 00:00:00
abstract：:The production of monoclonal antibodies (mAbs) to a common antigenic region on rabbit haemorrhagic disease virus (RHDV) has enabled the development of a capture ELISA for virus detection. The assay was shown to detect reliably the presence of viral antigen in crude homogenates of a range of rabbit tissues and has prov...
journal_title：Journal of virological methods
pub_type： 杂志文章
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更新日期：1996-04-26 00:00:00
abstract：:Noroviruses are the leading cause of nonbacterial gastroenteritis outbreaks in the United States, some of which are caused by the ingestion of contaminated water. Detection and genotypic characterization of noroviruses is commonly performed by reverse transcription-polymerase chain reaction (RT-PCR) followed by sequen...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2008.03.010
更新日期：2009-03-01 00:00:00
abstract：:A simple immunoperoxidase assay (IPA), adapted for detection of serum IgM antibodies to cytomegalovirus (CMV) is described. The antigen consisted of CMV infected human embryonic fibroblasts or isolated nuclei. The sera were absorbed with aggregated gamma-globulins prior to testing. Rabbit anti-human IgM peroxidase con...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(85)90122-3
更新日期：1985-05-01 00:00:00
abstract：:Rapid and accurate detection of measles virus is important for case diagnosis and public health management. This study compared the performance of two monoplex RT-PCR reactions targeting the H and N genes to a duplex RT-PCR targeting both genes simultaneously. The duplex simplified processing without compromising assa...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2016.11.003
更新日期：2017-01-01 00:00:00
abstract：:Real-time polymerase chain reaction (real-time PCR) is an accurate, rapid and reliable method that can be used for the detection and also for the quantitation of specific DNA molecules. It can be non-specific, with intercalating dyes (SYBR Green I dye) able to bind to any dsDNA, or specific with a probe (TaqMan), wher...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2012.05.010
更新日期：2012-09-01 00:00:00
