Abstract:
:A sensitive enzyme-linked immunosorbent assay (ELISA) has been developed which can detect serotype and group-specific antibodies (IgG) to fowl adenovirus serotypes 2, 3 and 4. The chickens produced principally type-specific antibodies after a single oral inoculation of virus which enabled that strain to be identified by the ELISA. However, inoculation of an heterologous serotype, although inducing strain-specific antibodies to itself, also induced high levels of antibody to the group-specific antigens. This masked the serotype-specific antibodies in the ELISA to the first serotype. The ELISA, which has similar sensitivity to the serum neutralisation test, could be used as a rapid, easily performed test to identify fowl adenovirus-specific antibodies.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Mockett AP,Cook JKdoi
10.1016/0166-0934(83)90086-1subject
Has Abstractpub_date
1983-12-01 00:00:00pages
327-35issue
5-6eissn
0166-0934issn
1879-0984pii
0166-0934(83)90086-1journal_volume
7pub_type
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journal_title:Journal of virological methods
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