The use of an enzyme-linked immunosorbent assay to detect IgG antibodies to serotype-specific and group-specific antigens of fowl adenovirus serotypes 2, 3 and 4.

Abstract:

:A sensitive enzyme-linked immunosorbent assay (ELISA) has been developed which can detect serotype and group-specific antibodies (IgG) to fowl adenovirus serotypes 2, 3 and 4. The chickens produced principally type-specific antibodies after a single oral inoculation of virus which enabled that strain to be identified by the ELISA. However, inoculation of an heterologous serotype, although inducing strain-specific antibodies to itself, also induced high levels of antibody to the group-specific antigens. This masked the serotype-specific antibodies in the ELISA to the first serotype. The ELISA, which has similar sensitivity to the serum neutralisation test, could be used as a rapid, easily performed test to identify fowl adenovirus-specific antibodies.

journal_name

J Virol Methods

authors

Mockett AP,Cook JK

doi

10.1016/0166-0934(83)90086-1

subject

Has Abstract

pub_date

1983-12-01 00:00:00

pages

327-35

issue

5-6

eissn

0166-0934

issn

1879-0984

pii

0166-0934(83)90086-1

journal_volume

7

pub_type

杂志文章
  • In situ hybridisation and in situ polymerase chain reaction detection of parvovirus B19 DNA within cells.

    abstract::Modification of an in situ polymerase chain reaction (ISPCR) technique is described for the detection of B19 parvovirus infection. Specific amplification of B19 DNA inside fixed cells was followed by hybridisation with a digoxigenin-labelled probe and then visualised by immunochemical reaction. The assay had higher se...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/0166-0934(94)90164-3

    authors: Gallinella G,Young NS,Brown KE

    更新日期:1994-12-01 00:00:00

  • Coupling generation of cytomegalovirus deletion mutants and amplification of viral BAC clones.

    abstract::Human cytomegalovirus (HCMV) genome manipulation has always been difficult. Recently, the introduction of full-length HCMV DNA into Escherichia coli as an artificial bacterial chromosome (BAC) clone has allowed reliable targeted mutagenesis. Here, we show the next generation of improvement in designing recombinant HCM...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2004.06.009

    authors: Wang W,Patterson CE,Yang S,Zhu H

    更新日期:2004-11-01 00:00:00

  • Clinical utility of 2',5'-oligoadenylate synthetase activity measurement: using whole blood as a highly sensitive method to detect the effects of IFN.

    abstract::Research progress on the pleiotropic effects of interferons (IFN) has thus far required detecting responses by weak IFN signals. The activity of 2',5'-oligoadenylate synthetase (2-5OAS) is a valuable indicator in the prognosis and IFN treatment of patients with viral diseases such as hepatitis B and C. Although serum ...

    journal_title:Journal of virological methods

    pub_type: 临床试验,杂志文章

    doi:10.1016/j.jviromet.2006.05.005

    authors: Uno K,Suginoshita Y,Kakimi K,Moriyasu Y,Nakano K,Nakamura N,Fujita T,Horino Y,Sato T,Kishida T

    更新日期:2006-09-01 00:00:00

  • Establishment and validation of an ELISA for the quantitation of HBsAg in recombinant hepatitis B vaccines.

    abstract::Commercial enzyme-linked immunosorbent assay (ELISA) kits for the determination of the in vitro potency of recombinant hepatitis B vaccines, which detect hepatitis B surface antigen (HBsAg), have been used frequently as an alternative for traditional in vivo potency tests. With the constant need for validation procedu...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2010.12.010

    authors: Costa CI,Delgado IF,da Costa JA,de Carvalho RF,Mouta Sda S Jr,Vianna CO,de Moraes MT

    更新日期:2011-03-01 00:00:00

  • Evaluation of the cobas® HCV test for quantifying HCV RNA in dried plasma spots collected using the cobas® Plasma Separation Card.

    abstract:OBJECTIVE:This study evaluated the performance of the cobas® hepatitis C virus (HCV) Test for use on the cobas® 6800/8800 Systems for the detection and quantification of HCV RNA collected using the cobas® Plasma Separation Card (PSC) compared with plasma samples. METHODS:Whole EDTA-venous blood was collected from 50 H...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2020.113820

    authors: Marins EG,Krey N,Becker A,Melzer S,Hoppler M

    更新日期:2020-04-01 00:00:00

  • Improved detection of simian immunodeficiency virus RNA by in situ hybridization in fixed tissue sections: combined effects of temperatures for tissue fixation and probe hybridization.

    abstract::In situ hybridization detection of viral RNAs in formaldehyde-fixed tissue specimens is used frequently to characterize the extent of viral replication within host tissues. The ability to determine the level of expression of viral RNAs in situ is dependent upon many factors including the extent of cross-linking during...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/s0166-0934(01)00378-0

    authors: Fallert BA,Reinhart TA

    更新日期:2002-01-01 00:00:00

  • Universal diagnostic RT-PCR protocol for arboviruses.

    abstract::A selected number of PCR protocols were evaluated to determine if they could serve as a universal protocol for detecting and identifying all arboviruses. In this study, four parameters that affect the efficacy of RT-PCR (RNA extraction method, choice of reverse transcriptase, choice of DNA polymerase and thermocycling...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/s0166-0934(98)00003-2

    authors: Kuno G

    更新日期:1998-05-01 00:00:00

  • Multiplex PCR for simultaneous detection and differentiation of sheeppox, goatpox and orf viruses from clinical samples of sheep and goats.

    abstract::A multiplex polymerase chain reaction (mPCR) was developed and evaluated for detection of pox viral infections simultaneously using clinical samples from sheep and goats. Specific primers for three pox viruses of sheep and goats including sheeppox virus (SPPV), goatpox virus (GTPV) and orf virus (ORFV) were designed t...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2013.10.009

    authors: Venkatesan G,Balamurugan V,Bhanuprakash V

    更新日期:2014-01-01 00:00:00

  • Coupling of foot-and-mouth disease virus to sheep red blood cells using tannic acid for immunological assays.

    abstract::A technique for coupling foot-and-mouth disease virus (FMDV) to tanned sheep red blood cells (SRBC) is reported. Different parameters influencing the procedure were studied. Subtypes C2, C3, O1 and A24 were used as antigens, and guinea pig hyperimmune sera obtained were tested for specific antibody in passive hemagglu...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/0166-0934(88)90131-0

    authors: Pujol CA,Feledi CA,Massouh EJ

    更新日期:1988-08-01 00:00:00

  • Optimization of allele-specific PCR using patient-specific HIV consensus sequences for primer design.

    abstract::Allele-specific PCR based on subtype consensus sequences is a powerful technique for detecting low frequency drug resistant mutants in HIV-1 infected patients. However, this approach can be limited by genetic variation in the region complementary to the primers, leading to variability in allele detection. The goals of...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2009.11.025

    authors: Boltz VF,Maldarelli F,Martinson N,Morris L,McIntyre JA,Gray G,Hopley MJ,Kimura T,Mayers DL,Robinson P,Mellors JW,Coffin JM,Palmer SE

    更新日期:2010-03-01 00:00:00

  • High frequency of mixed TT virus infections in healthy adults and children detected by a simplified heteroduplex mobility assay.

    abstract::Recombinant plasmids carrying 199 base pairs (bp) inserts from the non coding region (nucleotides (nt) 6-204) of the TT virus (TTV) genome were used to standardize an heteroduplex mobility assay able to detect mixed infections of a single individual with several TTV isolates. In this simplified heteroduplex mobility a...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/s0166-0934(01)00425-6

    authors: Saback FL,Gomes SA,Niel C

    更新日期:2002-03-01 00:00:00

  • Monoclonal antibody against Saint Louis encephalitis prM viral protein.

    abstract::Saint Louis encephalitis virus belongs to Flavivirus genus; Flaviviridae family jointly with other medically important flaviviruses including dengue virus and West Nile virus. The biological properties and functions of prM flavivirus protein are under investigation due to its importance in the generation of infectious...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2015.01.004

    authors: Pupo-Antúnez M,Vázquez S,Sosa AL,Caballero Y,Vásquez Y,Morier L,Álvarez M,Guzmán MG

    更新日期:2015-06-15 00:00:00

  • WNV Typer: a server for genotyping of West Nile viruses using an alignment-free method based on a return time distribution.

    abstract::West Nile virus (WNV), genus Flavivirus, family Flaviviridae, is a major cause of viral encephalitis with broad host range and global spread. The virus has undergone a series of evolutionary changes with emergence of various genotypic lineages that are known to differ in type and severity of the diseases caused. Curre...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2013.12.012

    authors: Kolekar P,Hake N,Kale M,Kulkarni-Kale U

    更新日期:2014-03-01 00:00:00

  • Extraction and purification of hepatitis B virus-like M particles from a recombinant Saccharomyces cerevisiae strain using alumina powder.

    abstract::A recombinant hepatitis B surface antigen (HBsAg) has been produced in the yeast Saccharomyces cerevisiae and used as a vaccine against hepatitis B virus (HBV) infection. The present study aimed to optimize the extraction of recombinant virus-like particles (rVLPs) to develop a simple purification procedure based on g...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2012.09.023

    authors: Hadiji-Abbes N,Martin M,Benzina W,Karray-Hakim H,Gergely C,Gargouri A,Mokdad-Gargouri R

    更新日期:2013-01-01 00:00:00

  • Simplified dengue virus microwell plaque assay using an automated quantification program.

    abstract::The plaque assay is essential for virion quantitation but the classic protocol requires considerable efforts. A simplified dengue 96-well plaque assay with automated quantitation program is an alternative to access the level of infectious virus. Dengue plaque assay was simplified using LLC/MK2 cells and virus mixing s...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2016.08.009

    authors: Boonyasuppayakorn S,Suroengrit A,Srivarangkul P,Yuttithamnon W,Pankaew S,Saelee T,Prompetchara E,Salakij S,Bhattarakosol P

    更新日期:2016-11-01 00:00:00

  • Detection of adenovirus by rapid 24-well plate centrifugation and conventional cell culture with dexamethasone.

    abstract::Two methods for rapid detection of adenovirus were tested: (i) 24-well plate centrifugation followed by staining with a monoclonal antibody after incubation for 24 h and 48 h, and (ii) pretreatment of A549 cells used in conventional cell culture and 24-well plate centrifugation with 10(-5)M dexamethasone. Twenty-seven...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/0166-0934(88)90144-9

    authors: Woods GL,Yamamoto M,Young A

    更新日期:1988-06-01 00:00:00

  • Baculovirus expressed 2C of foot-and-mouth disease virus has the potential for differentiating convalescent from vaccinated animals.

    abstract::Determining whether animals have been infected with foot-and-mouth disease virus or vaccinated is important because infected animals frequently become carriers of the virus, shed it intermittently and thus may be the source of new outbreaks of the disease. We had shown previously that the sera of convalescent animals ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/s0166-0934(96)02165-9

    authors: Meyer RF,Babcock GD,Newman JF,Burrage TG,Toohey K,Lubroth J,Brown F

    更新日期:1997-04-01 00:00:00

  • Development of a generic real-time PCR assay for simultaneous detection of proviral DNA of simian Betaretrovirus serotypes 1, 2, 3, 4 and 5 and secondary uniplex assays for specific serotype identification.

    abstract::Simian betaretroviruses (formerly Type D retroviruses; SRV) are a group of closely related retroviruses for which the natural host species are Asian monkeys of the genus Macaca. Five serotypes have been identified by classical neutralization assays and three additional untyped variants have been reported (SRV(Tsukuba)...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2009.07.030

    authors: White JA,Todd PA,Rosenthal AN,Yee JL,Grant R,Lerche NW

    更新日期:2009-12-01 00:00:00

  • One-step real-time PCR assay for detection and quantitation of hepatitis D virus RNA.

    abstract::Hepatitis D virus (HDV) is a defective virus which requires hepatitis B virus (HBV) surface antigen (HBsAg) for its assembly. Hepatitis B infected individuals co-infected or superinfected with HDV often present with more severe hepatitis, progress faster to liver disease, and have a higher mortality rate than individu...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2013.07.033

    authors: Kodani M,Martin A,Mixson-Hayden T,Drobeniuc J,Gish RR,Kamili S

    更新日期:2013-11-01 00:00:00

  • Comparison of ELISA and RT-PCR for the detection of Prunus necrotic ring spot virus and prune dwarf virus in almond (Prunus dulcis).

    abstract::A technique based on the reverse transcriptase-polymerase chain reaction (RT-PCR) has been developed to detect the presence of Prunus necrotic ringspot virus (PNRSV) and prune dwarf virus (PDV) simultaneously in almond. This paper presents the results of a 3-year study comparing both enzyme-linked immunosorbent assay ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2003.08.014

    authors: Mekuria G,Ramesh SA,Alberts E,Bertozzi T,Wirthensohn M,Collins G,Sedgley M

    更新日期:2003-12-01 00:00:00

  • Disparate detection outcomes for anti-HCV IgG and HCV RNA in dried blood spots.

    abstract::Dried blood spots (DBS) expedite the collection, storage and shipping of blood samples, thereby facilitating large-scale serologic studies. We evaluated the sensitivity of anti-HCV IgG testing and HCV-RNA quantitation using freshly prepared and stored DBS derived from HCV-infected patients. Protocols for elution were ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2014.10.018

    authors: Tejada-Strop A,Drobeniuc J,Mixson-Hayden T,Forbi JC,Le NT,Li L,Mei J,Terrault N,Kamili S

    更新日期:2015-02-01 00:00:00

  • The use of markers in immune electron microscopy.

    abstract::Immune electron microscopy (IEM) cannot be used successfully for structures that do not have recognisable morphology. However, at least some of these structures or components are related antigenically to recognisable antigens or viruses. We have therefore mixed unknown antigens with known markers and looked for the pr...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/0166-0934(81)90035-5

    authors: Almeida JD,Skelly J,Howard CR,Zuckerman AJ

    更新日期:1981-02-01 00:00:00

  • Solid-phase enzyme immunoassay for rotavirus antigen: faecal protease activity as a reason for false-negative results.

    abstract::Rabbits and guinea pigs were immunized with purified bovine rotavirus. Immunoglobulin G fractions of the resulting antisera were used in a standard four-layer solid-phase enzyme immunoassay (EIA) for rotavirus antigen in human faecal specimens. Samples negative for rotavirus in electron microscopy, when diluted in sta...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/0166-0934(82)90096-9

    authors: Hovi T,Väisänen V,Ukkonen P,von Bonsdorff CH

    更新日期:1982-09-01 00:00:00

  • Detection of Borna disease virus RNA in naturally infected animals by a nested polymerase chain reaction.

    abstract::Borna disease virus in naturally infected horses, a donkey and sheep was detected for the first time by amplification of viral RNA using PCR. In contrast to a control group of healthy horses, brain tissue was positive by this assay in all animals with neurological symptoms. The use of a second round of PCR with nested...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/0166-0934(94)90098-1

    authors: Zimmermann W,Dürrwald R,Ludwig H

    更新日期:1994-02-01 00:00:00

  • Development of automated immunoassays for immune status screening and serodiagnosis of rubella virus infection.

    abstract::The fully automated IMx immunoassay analyzer was used to develop a system for the detection of IgG and IgM antibodies to rubella virus for immune status screening and diagnosis of primary infections. Reagents and assay protocol software were developed using rubella virus sensitized microparticles as the solid phase to...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/0166-0934(90)90139-7

    authors: Abbott GG,Safford JW,MacDonald RG,Craine MC,Applegren RR

    更新日期:1990-02-01 00:00:00

  • Simultaneous detection of hemagglutinin and neuraminidase genes of novel influenza A (H7N9) by duplex real-time reverse transcription polymerase chain reaction.

    abstract::A novel reassortant influenza A (H7N9) virus emerged recently in China. In this study, a duplex real-time reverse transcription polymerase chain reaction (rRT-PCR) assay was developed for the simultaneous detection of hemagglutinin (HA) and neuraminidase (NA) genes of H7N9 influenza viruses. The sensitivity of the ass...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2013.08.021

    authors: Li Y,Wu T,Qi X,Ge Y,Guo X,Wu B,Yu H,Zhu Y,Shi Z,Wang H,Cui L,Zhou M

    更新日期:2013-12-01 00:00:00

  • Quantification of recombinant core-like particles of bluetongue virus using immunosorbent electron microscopy.

    abstract::Immunosorbent electron microscopy was used to quantify recombinant baculovirus-generated bluetongue virus (BTV) core-like particles (CLP) in either purified preparations or lysates of recombinant baculovirus-infected cells. The capture antibody was an anti-BTV VP7 monoclonal antibody. The CLP concentration in purified...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/s0166-0934(98)00170-0

    authors: Zheng YZ,Hyatt A,Wang LF,Eaton BT,Greenfield PF,Reid S

    更新日期:1999-06-01 00:00:00

  • Simultaneous detection of enterovirus 70 and coxsackievirus A24 variant by multiplex real-time RT-PCR using an internal control.

    abstract::Epidemics of acute hemorrhagic conjunctivitis are always explosive and extensive, and have been recognized as a serious international public health problem. Enterovirus 70 and coxsackievirus A24 variant have been identified as the major etiological agents in acute hemorrhagic conjunctivitis outbreaks worldwide. A nove...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2009.02.022

    authors: Xiao XL,Wu H,Li YJ,Li HF,He YQ,Chen G,Zhang JW,Yang H,Li XF,Yang XQ,Yu YG

    更新日期:2009-07-01 00:00:00

  • A novel EBNA-1 titration method and putative anti-EBNA-1 protein.

    abstract::A novel and rapid EBNA-1 titration method has been developed which uses immunoprecipitation of specific DNA-protein complexes with EBNA-1-positive serum. The method is more sensitive than the conventional immunofluorescence method and has potential value as a diagnostic reagent for clinical laboratories. TPA induction...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/0166-0934(88)90062-6

    authors: Nonoyama M,Wen LT,Tabata T,Tanaka A

    更新日期:1988-09-01 00:00:00

  • Immunodiagnosis of Prune dwarf virus using antiserum produced to its recombinant coat protein.

    abstract::Certification represents the first line of defense against fruit tree viruses. For certification or surveys dealing with large number of samples, ELISA is still considered the technique of choice and requires a continuous supply of good quality antibodies. Prune dwarf virus (PDV) is among the major viruses affecting s...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2004.05.013

    authors: Abou-Jawdah Y,Sobh H,Cordahi N,Kawtharani H,Nemer G,Maxwell DP,Nakhla MK

    更新日期:2004-10-01 00:00:00