Abstract：
:Adequate treatment of influenza requires identification of viral type as well as detection of mutation(s) conferring drug resistance. Reverse hybridization-based line probe assays (LiPA) can be performed using several probes immobilized on nitrocellulose, strips enabling LiPA to determine simultaneously viral subtypes and detect the presence or absence of the H274Y mutation, which confers oseltamivir resistance of H1N1 influenza viruses. LiPA was developed for identification of H1N1 influenza virus subtypes (pandemic 2009 and seasonal types), as well as H3N2 and B subtypes, and to detect the H274Y mutation. The diagnostic capability of this assay was evaluated using cultured virus isolates as well as nasal swabs obtained from patients suspected of infection with influenza. In examining 354 cultured virus isolates, the LiPA showed 100% specificity for virus typing and 99% specificity for detecting the H274Y mutation. In 49 nasal swabs from a clinical study, the assay showed 100% specificity for virus typing and 88% specificity for detecting the absence of the H274Y mutation, although none of these swabs was PCR-positive for this mutation. These findings indicate that LiPA for influenza viruses may be used to monitor viral trends during the influenza season.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Miyoshi-Akiyama T,Akasaka Y,Oogane T,Kondo Y,Matsushita T,Funatogawa K,Kirikae Tdoi
10.1016/j.jviromet.2012.06.018subject
Has Abstractpub_date
2012-11-01 00:00:00pages
276-80issue
2eissn
0166-0934issn
1879-0984pii
S0166-0934(12)00231-5journal_volume
185pub_type
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