Abstract：
:Genetic reassortment of influenza viruses is widely used for creating viruses with specific phenotypes. Reassortment of two influenza viruses, each with eight RNA segments potentially yields as many as 256 gene segment combinations. Therefore, confirmation that progeny viruses possess genomes corresponding to the specified phenotypes can be laborious and time-consuming. To establish a convenient method for genotyping influenza virus reassortants, we adapted single-strand conformation polymorphism analysis (SSCP) using standard laboratory equipment. By varying the concentration of polyacrylamide between 4-6% and the concentration of glycerol between 5-8% in the gel, together with adding PCR primers to the DNA sample during the denaturing step, optimal conditions can be found for SSCP with little effort. The described method has high accuracy and reliability, and provides a tool for rapid, cost-effective genetic screening and assessment of the purity and genetic stability of the reassortant viruses. This method should be useful in basic research applications and in preparing reassortant viruses for vaccine use.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Lugovtsev VY,Vodeiko GM,Strupczewski CM,Levandowski RAdoi
10.1016/j.jviromet.2004.11.024subject
Has Abstractpub_date
2005-03-01 00:00:00pages
203-10issue
1-2eissn
0166-0934issn
1879-0984pii
S0166-0934(04)00376-3journal_volume
124pub_type
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
pub_type： 杂志文章
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更新日期：2021-02-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
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journal_title：Journal of virological methods
pub_type： 杂志文章
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journal_title：Journal of virological methods
pub_type： 杂志文章
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2010.04.019
更新日期：2010-09-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Journal of virological methods
pub_type： 杂志文章
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
pub_type： 杂志文章
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