Detection of herpes simplex virus by measurement of luciferase activity in an infected-cell lysate.

abstract：:The genus Caulimovirus consists of several distinct virus species with a double-stranded DNA genome that infect diverse plant species. A comparative analysis of the sequences of known Caulimovirus species revealed two regions that are conserved in all Caulimovirus species with the exception of Strawberry vein banding ...

journal_title：Journal of virological methods

authors： Pappu HR,Druffel KL

更新日期：2009-04-01 00:00:00

abstract：:One of the most important steps when preparing a live attenuated vaccine is the assessment of the level of attenuation in target animals. It is costly and time consuming as it requires, on each occasion, a large number of susceptible animals and contained accommodation. This study assessed the consistency of the bovin...

journal_title：Journal of virological methods

authors： Franchi P,Mercante MT,Ronchi GF,Armillotta G,Ulisse S,Molini U,Di Ventura M,Lelli R,Savini G,Pini A

更新日期：2008-11-01 00:00:00

abstract：:To develop reverse genetics system of RNA viruses, cloning of full-length viral genome is required which is often challenging due to many steps involved. In this study, we report cloning of full-length cDNA from an Indian field isolate (CSFV/IVRI/VB-131) of classical swine fever virus (CSFV) using in vitro overlap ext...

journal_title：Journal of virological methods

authors： Kamboj A,Saini M,Rajan LS,Patel CL,Chaturvedi VK,Gupta PK

更新日期：2015-12-15 00:00:00

abstract：:As a prelude to the development of nucleic acid probes specific for non-A, non-B hepatitis virus(es) (NANBV), plasmas with alanine aminotransferase levels greater than or equal to 110 IU were assayed for DNA by a radioimmunoassay. Approximately 50% of such plasmas are expected to contain NANBV. One-hundred and seventy...

journal_title：Journal of virological methods

authors： Neurath AR,Strick N,Miller K,Waldman AA

更新日期：1984-02-01 00:00:00

abstract：:Polymerase chain reaction (PCR) assays were developed for the detection of RNA from the St. Louis encephalitis (SLE) virus. Using computer-assisted analysis of the MSI-7 strain SLE virus genome, two primer pairs were selected from the capsid-coding and the membrane associated protein-coding genes, and one from the env...

journal_title：Journal of virological methods

authors： Howe DK,Vodkin MH,Novak RJ,Shope RE,McLaughlin GL

更新日期：1992-01-01 00:00:00

abstract：:Congenital human cytomegalovirus infections are the major infectious cause of birth defects in the United States. How this virus crosses the placenta and causes fetal disease is poorly understood. Guinea pig cytomegalovirus (GPCMV) is a related virus that provides an important model for studying cytomegaloviral congen...

journal_title：Journal of virological methods

authors： Cui X,McGregor A,Schleiss MR,McVoy MA

更新日期：2008-05-01 00:00:00

abstract：:A rapid rate-kinetic turbidometric assay for the quantitation of viral complement fixing antibodies has been developed, using adenovirus as a model. The procedure is based on the turbidometric quantitation of intact sheep erythrocytes and measures the rate of hemolysis (change in absorbance at 640 nm/min/), at maximum...

journal_title：Journal of virological methods

authors： Fulton RE,Dininno VL

更新日期：1985-10-01 00:00:00

abstract：:The development of a rapid and highly sensitive PCR assay for the detection of human rhinoviruses (HRVs) in nasopharyngeal aspirates is described. Two simple and fast commercial RNA extraction methods and four primer pairs were compared. The most sensitive RNA extraction method (Ultraspec) and primer pair (A) were app...

journal_title：Journal of virological methods

authors： Santti J,Hyypiä T,Halonen P

更新日期：1997-06-01 00:00:00

abstract：:Double-stranded RNA (dsRNA) is in many cases the only available template for molecular and diagnostic studies of RNA viruses. A novel mycovirus with a five dsRNAs segmented-genome served as a model system for the amplification and cloning of dsRNA segments using several PCR-based methods. Sequences obtained by the cla...

journal_title：Journal of virological methods

authors： Darissa O,Willingmann P,Adam G

更新日期：2010-11-01 00:00:00

abstract：:A high-throughput method of isolating and cloning geminivirus genomes from dried plant material, by combining an Extract-n-Amp-based DNA isolation technique with rolling circle amplification (RCA) of viral DNA, is presented. Using this method an attempt was made to isolate and clone full geminivirus genomes/genome com...

journal_title：Journal of virological methods

authors： Shepherd DN,Martin DP,Lefeuvre P,Monjane AL,Owor BE,Rybicki EP,Varsani A

更新日期：2008-04-01 00:00:00

abstract：:Lumpy skin disease virus (LSDV) is a poxvirus that causes systemic disease in cattle, resulting in substantial economic loss to affected communities. LSDV is a rapidly emerging pathogen of growing global concern that recently spread from Africa and the Middle East into Europe and Asia, impacting the cattle population ...

journal_title：Journal of virological methods

authors： Fay PC,Cook CG,Wijesiriwardana N,Tore G,Comtet L,Carpentier A,Shih B,Freimanis G,Haga IR,Beard PM

更新日期：2020-11-01 00:00:00

abstract：:Marek's disease virus (MDV) is a highly oncogenic alphaherpesvirus that induces rapid-onset T-cell lymphoma in poultry. The complete genome of the avirulent vaccine strain MDV-814 was cloned as an infectious bacterial artificial chromosome (BAC) using an 8.8-kb fragment containing the self-designed selective marker gu...

journal_title：Journal of virological methods

authors： Cui HY,Wang YF,Shi XM,An TQ,Tong GZ,Lan DS,He L,Liu CJ,Wang M

更新日期：2009-03-01 00:00:00

abstract：:Grapevine shoot cultures infected by Grapevine vitivirus A (GVA) were grown on Quorin-Lepoivre basic medium and submitted to in vitro chemotherapy and thermotherapy sanitation techniques. Ribavirin (Rb) at 20gml(-1), dihydroxypropyladenine (DHPA) at 60gml(-1) and their combination (RbDH) were added to the proliferatin...

journal_title：Journal of virological methods

authors： Panattoni A,D'Anna F,Cristani C,Triolo E

更新日期：2007-12-01 00:00:00

abstract：:Hepatitis E virus (HEV) is the causative agent of hepatitis E, a food- and water-borne disease. In developed countries, consumption of meats from pigs, wild boars and deer is a major source of infection. Although HEV and HEV-related viruses have been detected in many animal species, their zoonotic potential and preval...

journal_title：Journal of virological methods

authors： Yonemitsu K,Terada Y,Kuwata R,Nguyen D,Shiranaga N,Tono S,Matsukane T,Yokoyama M,Suzuki K,Shimoda H,Takano A,Muto M,Maeda K

更新日期：2016-12-01 00:00:00

abstract：:Rapid detection of CMV-DNA in urine specimens by dot-blot hybridization was compared to conventional virus isolation and to virus identification using solid-phase immunoelectron microscopy (SPIEM). To detect viral DNA, 32P-labeled EcoR1 J fragment of CMV-DNA was used as a probe in the hybridization assay. In addition,...

journal_title：Journal of virological methods

authors： Vonsover A,Gotlieb-Stematsky T,Sayar Y,Bardov L,Manor Y,Siegal B

更新日期：1987-05-01 00:00:00

abstract：:Studies were performed to identify a pair of primers, specific for the tick-borne encephalitis (TBE) virus complex of the Flaviviridae, with which to develop a rapid and specific identification system based on reverse transcription and the polymerase chain reaction (RT-PCR). The specificity of a putative primer pair w...

journal_title：Journal of virological methods

authors： Whitby JE,Ni H,Whitby HE,Jennings AD,Bradley LM,Lee JM,Lloyd G,Stephenson JR,Barrett AD

更新日期：1993-11-01 00:00:00

abstract：:Expression of recombinant beak and feather disease virus (BFDV) capsid-associated protein (Cap) has relied on inefficient techniques that typically produce low yields or use specialized expression systems, which greatly increase the cost and expertise required for mass production. An Escherichia coli system was used t...

journal_title：Journal of virological methods

authors： Patterson EI,Swarbrick CM,Roman N,Forwood JK,Raidal SR

更新日期：2013-04-01 00:00:00

abstract：:MicroRNAs (miRNAs) are a large class of endogenous approximately 22-nucleotide long non-coding RNAs involved in post-transcriptional silencing of gene expression by translational repression or direct cleavage of the target mRNAs. Several hundreds of miRNAs have now been identified in a wide range of organisms includin...

journal_title：Journal of virological methods

authors： Xu H,Yao Y,Zhao Y,Smith LP,Baigent SJ,Nair V

更新日期：2008-05-01 00:00:00

abstract：:A sensitive and specific blocking enzyme-linked immunosorbent assay (ELISA) was developed to distinguish infectious bovine rhinotracheitis virus (IBRV)-infected animals from those immunized with a glycoprotein gIII deletion mutant, IBRV(NG)dltkdlgIII. For this ELISA, undiluted test sera are used to block the binding o...

journal_title：Journal of virological methods

authors： Kit S,Otsuka H,Kit M

更新日期：1992-10-01 00:00:00

abstract：OBJECTIVE:This study evaluated the performance of the cobas® hepatitis C virus (HCV) Test for use on the cobas® 6800/8800 Systems for the detection and quantification of HCV RNA collected using the cobas® Plasma Separation Card (PSC) compared with plasma samples. METHODS:Whole EDTA-venous blood was collected from 50 H...

journal_title：Journal of virological methods

authors： Marins EG,Krey N,Becker A,Melzer S,Hoppler M

更新日期：2020-04-01 00:00:00

abstract：:A novel DNA detection assay comprising Hybrid Capture sample preparation, GP5+/6+ PCR with modifications and Luminex 100 detection was developed and applied to genotyping of human papillomavirus (HPV) in cervical samples. Target-specific sample preparation was performed using magnetic beads conjugated with Hybrid Capt...

journal_title：Journal of virological methods

authors： Nazarenko I,Kobayashi L,Giles J,Fishman C,Chen G,Lorincz A

更新日期：2008-12-01 00:00:00

abstract：:Feline infectious peritonitis (FIP) is a fatal disease in cats worldwide. The aim of this study was to test two commercially available reaction mixtures in a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay to detect feline Coronavirus (FCoV) in body cavity effusions of cats with and withou...

journal_title：Journal of virological methods

authors： Günther S,Felten S,Wess G,Hartmann K,Weber K

更新日期：2018-06-01 00:00:00

abstract：:Sample preparation is an important step in the detection of viral DNA by the polymerase chain reaction (PCR) technique. The method used should achieve release of cellular DNA with the minimum of manipulation steps so as to reduce the possibility of contamination. The present report demonstrates that either microwaving...

journal_title：Journal of virological methods

authors： Lou YK,Qin H,Molodysky E,Morris BJ

更新日期：1993-09-01 00:00:00

abstract：:Contaminated recreational waters pose a public health concern, as the potential for waterborne diseases exists in water contaminated with human fecal waste. Worldwide, bacterial indicators such as Escherichia coli, enterococci, and total and fecal coliform are used as indicators of water quality. However, enteric viru...

journal_title：Journal of virological methods

authors： Allmann E,Pan L,Li L,Li D,Wang S,Lu Y

更新日期：2013-11-01 00:00:00

abstract：:Tomato necrotic stunt virus (ToNStV) is an emerging potyvirus that causes severe stunting to infected tomato plants. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for sensitive detection of ToNStV. The sensitivity of RT-LAMP was comparable to that of conventional RT-PCR, ...

journal_title：Journal of virological methods

authors： Li R,Ling KS

更新日期：2014-05-01 00:00:00

abstract：:This study describes the development and evaluation of a cost effective test rationale for the detection of anti-HCV in dried blood spots. Samples were screened using an 'in house' IgG ELISA that incorporated the recombinant proteins c22-3, c200 and NS5. Confirmation of specific antibody to HCV was by a modification o...

journal_title：Journal of virological methods

authors： Parker SP,Cubitt WD,Ades AE

更新日期：1997-11-01 00:00:00

abstract：:Diagnostic tests are usually evaluated in terms of simple qualitative measures of sensitivity and specificity. When comparing different quantitative assays such as ELISAs, it is often more useful to deal with actual values (sample optical density/cut-off optical density ratio (OD ratio] rather than the qualitative rel...

journal_title：Journal of virological methods

authors： Crofts N,Maskill W,Gust ID

更新日期：1988-10-01 00:00:00

abstract：:Nipah virus, a zoonotic paramyxovirus which emerged recently was chemically inactivated using binary ethylenimine (BEI). The inactivated virus was concentrated and purified by sucrose gradient centrifugation. The gradient fractions were examined by electron microscopy and Western immunoblot, and gradient fraction cont...

journal_title：Journal of virological methods

authors： Berhane Y,Berry JD,Ranadheera C,Marszal P,Nicolas B,Yuan X,Czub M,Weingartl H

更新日期：2006-03-01 00:00:00

abstract：:The consumption of bivalve shellfish is a common cause of foodborne outbreaks of viral origin. The evaluation of the sanitary quality of these products, however, is still based on bacterial indicators of fecal contamination (Reg. (EC) No. 2073/2005 and No.1441/2007) even if it is known that they are not reliable indic...

journal_title：Journal of virological methods

authors： Di Pasquale S,Paniconi M,De Medici D,Suffredini E,Croci L

更新日期：2010-01-01 00:00:00

abstract：:Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed livestock which has a drastic economic impact for affected countries. Although FMDV is distributed worldwide, many regional programs have been effective eradicating this agent. In Argentina, as in many other regions of South America, the comb...

journal_title：Journal of virological methods

authors： Jaworski JP,Compaired D,Trotta M,Perez M,Trono K,Fondevila N

更新日期：2011-12-01 00:00:00