解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Extracts of Salvia species are used in traditional medicine to treat various diseases. The economic importance of this genus has increased in recent years due to evidence that some of its secondary metabolites have valuable pharmaceutical and nutraceutical properties.The bioactivity of sage extracts is mainly due to t...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-013-1350-z
更新日期:2014-02-01 00:00:00
abstract::Cellobiose is a major intermediate from cellulase hydrolysis of pretreated plant biomass. Engineering biocatalysts for direct use of cellobiose could eliminate the need for exogenous β-glucosidase. Additionally, rapid removal of cellobiose in a simultaneous saccharification and fermentation facilitates enzymatic hydro...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1355-7
更新日期:2014-02-01 00:00:00
abstract::The nitrilase from Rhodococcus sp. CCZU10-1 catalyses the hydrolysis of dinitriles to acids without the formation of amides and cyanocarboxylic acids. It was induced by benzonitrile and its analogues (tetrachloroterephthalonitrile > ε-caprolactam > benzonitrile > phenylacetonitrile), and had activity towards aromatic ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1367-3
更新日期:2014-02-01 00:00:00
abstract::miR-21 is highly expressed in a variety of cancers, suggesting that it might play a role in the process of oncogenesis, as supported by it directly causing pre-B cell lymphomas in transgenic mice. Rm21LG transgenic mice for the conditional co-expression of miR-21 and luciferase (Luc) mediated by Cre/lox P system were ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1327-y
更新日期:2014-01-01 00:00:00
abstract::A fully contained and efficient heterologous protein production system was designed using Brassica rapa rapa (turnip) hairy roots. Two expression cassettes containing a cauliflower mosaic virus (CaMV) 35S promoter with a duplicated enhancer region, an Arabidopsis thaliana sequence encoding a signal peptide and the CaM...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1335-y
更新日期:2014-01-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) is a heterodimer containing 66 kDa p66 and 51 kDa p51 subunits. We previously showed that HIV-1 group M (HIV-1 M) RT and HIV-1 group O (HIV-1 O) RT have higher affinities for dTTP and template-primer (T/P) than Moloney murine leukemia virus RT, whi...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1321-4
更新日期:2013-12-01 00:00:00
abstract::Pancreatic islet transplantation is used to treat diabetes mellitus that has minimal complications and avoids hypoglycemic shock. Conformal microencapsulation of pancreatic islets improves their function by blocking immunogenic molecules while protecting fragile islets. However, production of empty alginate capsules d...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1300-9
更新日期:2013-12-01 00:00:00
abstract::Peptides are emerging as pharmaceutical agents in cancer therapy. The peptide, TLSGAFELSRDK (TLS) is a targeting ligand that can specifically triggers cellular uptake by binding to SKOV3 cells. Cell surface proteins and the C-terminal basic residues of the TLS are required for effective cell penetration, and the uptak...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1263-x
更新日期:2013-11-01 00:00:00
abstract::A brown-rot fungus, Fomitopsis pinicola, degraded polyvinyl alcohol (PVA) in quartz sand but not in liquid culture. From gel permeation chromatography analysis, the high-molecular-weight fraction of PVA was decreased by the action of F. pinicola but the coloration of the culture filtrate with I2 solution increased. Th...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1281-8
更新日期:2013-11-01 00:00:00
abstract::A gene-specific, metagenomic PCR method has led to the discovery of a novel esterase subfamily consisting of five homologous members. Sequence analysis of this esterase subfamily, named the ArmEst subfamily, revealed a unique conserved pattern with a significant variable interior sequence flanked by two symmetric and ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1293-4
更新日期:2013-11-01 00:00:00
abstract::A purified recombinant enzyme from Spirochaeta thermophila, that is suggested to be a cellobiose 2-epimerase, was a 47 kDa monomer with a specific activity of 29.2 U min(-1) for mannobiose. The epimerization activity of the recombinant enzyme for mannobiose was maximal at pH 7.0 and 60 °C with a half-life of 124 h. Th...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1267-6
更新日期:2013-11-01 00:00:00
abstract::The proteome of rumen epithelial tissue was analysed by SDS-PAGE coupled with LC-MS/MS. 813 non-redundant proteins were identified of which 7.4 % featured membrane-spanning domains and 15.4 % harboured a signal peptide. According to the gene ontology annotation, the most abundant proteins exhibited binding activities ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1291-6
更新日期:2013-11-01 00:00:00
abstract:PURPOSE OF WORK:The purpose of this study is to report a ι-carrageenase which degrades ι-carrageenan yielding neo-ι-carratetraose as the main product in the absence of NaCl. The gene for a new ι-carrageenase, CgiB_Ce, from Cellulophaga sp. QY3 was cloned and sequenced. It comprised an ORF of 1,386 bp encoding for a pro...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1244-0
更新日期:2013-10-01 00:00:00
abstract::16,17-Epoxysterol plays an important role in pharmaceutical steroid synthesis. To investigate the potential application of cytochrome P450 for epoxysterol synthesis, an approach to the epoxidation of 16,17-epoxysterol, based on directed evolution of cytochrome P450 BM-3, was developed. This comprised random gene mutag...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1254-y
更新日期:2013-10-01 00:00:00
abstract::There has been an increasing interest in applying biotechnology in formulating and characterising new and innovative drug delivery methods, e.g., drug-loaded biodegradable microneedles within the area of transdermal delivery technology. Recently, microneedles have been proposed for use in pain management, e.g., post-o...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-013-1217-3
更新日期:2013-09-01 00:00:00
abstract::Two ketoreductases from Candida glabrata were used for the asymmetric reduction of prochiral substituted acetophenones displayed different enantiopreference toward para-, meta-substituted and ortho-halogen substituted acetophenones with excellent enantioselectivity. Homology modeling and docking analysis were in confo...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1228-0
更新日期:2013-09-01 00:00:00
abstract::Ramoplanin is a lipopeptide antibiotic active against multi-drug-resistant, Gram-positive pathogens. Structurally, it contains a di-mannose moiety attached to the peptide core at Hpg(11). The biosynthetic gene cluster of ramoplanin has already been reported and the assembly of the depsipeptide has been elucidated but ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1233-3
更新日期:2013-09-01 00:00:00
abstract::δ-Integration can improve the expression stability and increase the copy number of exogenous genes in Saccharomyces cerevisiae. Generally, δ-integration vectors employ auxotrophic markers, such as LEU2-d, HIS3, TRP1, and URA3, to screen transformants. In this study, two cellulase genes (β-glucosidase and endoglucanase...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1205-7
更新日期:2013-08-01 00:00:00
abstract::A carboxylesterase gene from thermophilic bacterium, Alicyclobacillus tengchongensis, was cloned and expressed in Escherichia coli BL21 (DE3). The gene coded for a 513 amino acid protein with a calculated molecular mass of 57.82 kDa. The deduced amino acid sequence had structural features highly conserved among serine...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1195-5
更新日期:2013-08-01 00:00:00
abstract::Aptamers against inactive Vibrio alginolyticus were selected from an 82-nt ssDNA random library by systematic evolution of ligands by exponential enrichment. After 15 rounds of selection, the final pool of aptamers was highly specific for inactivated V. alginolyticus and had a dissociation constant of 27.5 ± 9.2 nM. U...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1154-1
更新日期:2013-06-01 00:00:00
abstract::Genomics, transcriptomics, proteomics and fluxomics are powerful omics-technologies that play a major role in today's research. For each of these techniques good sample quality is crucial. Major factors contributing to the quality of a sample is the actual sampling procedure itself and the way the sample is stored dir...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1163-0
更新日期:2013-06-01 00:00:00
abstract::Enzymatic polymerization can offer metal-free routes to polymer materials that could be used in biomedical applications. To take advantage of the unique properties of ionic liquids (ILs) for enzyme stability, monocationic ionic liquid (MIL) and dicationic ionic liquid (DIL) were used to promote the ring-opening polyme...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1160-3
更新日期:2013-06-01 00:00:00
abstract::Biotransformation of 1,3-dichloro-2-propanol (DCP) to epichlorohydrin (ECH) by the whole cells of recombinant Escherichia coli expressing halohydrin dehalogenase was limited by product inhibition. To solve this problem and improve the ECH yield, a biotransformation strategy using resin-based in situ product removal (I...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1165-y
更新日期:2013-06-01 00:00:00
abstract::The industrial-scale production of phenylalanine ammonia-lyase (PAL) mainly uses strains of Rhodotorula. However, the PAL gene from Rhodotorula has not been cloned. Here, the full-length gene of PAL from Rhodotorula glutinis was isolated. It was 2,121 bp, encoding a polypeptide with 706 amino acids and a calculated MW...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1140-7
更新日期:2013-05-01 00:00:00
abstract::Virus-induced gene silencing is based on the sequence-specific degradation of RNA. Here, a gene silencing vector derived from EuMV-YP, named pEuMV-YP:ΔAV1, was used to silence ChlI and NPR1 genes in Nicotiana benthamiana. The silencing of the ChlI transcripts was efficient in the stems, petioles and leaves as reflecte...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1146-1
更新日期:2013-05-01 00:00:00
abstract::The construction and testing of a unique instrument, the Paleobiosphere, which mimics some of the conditions of the ancient earth, is described. The instrument provides an experimental testing system for determining if certain microbes, when provided an adequate environment, can degrade biological materials to produce...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-1123-0
更新日期:2013-04-01 00:00:00
abstract::UV-induced DNA damage plays a key role in the etiology of certain diseases. The ability of blueberry anthocyanins and anthocyanidins (BA) to protect cellular DNA from UV-induced damage was investigated. BA were extracted by water (BAW), ethanol (BAE) or methanol (BAM). These extracts partially restored proliferation o...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-1105-2
更新日期:2013-04-01 00:00:00
abstract::A novel epoxide hydrolase (EHase) from polycyclic aromatic hydrocarbon (PAH)-degrading bacteria was identified and characterized. EHase activity was identified in four strains of PAH-degrading bacteria isolated from commercial gasoline and oil-contaminated sediment based on their growth on styrene oxide and its deriva...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-1114-1
更新日期:2013-04-01 00:00:00
abstract::The gene encoding a novel short-chain alcohol dehydrogenase in the thermophilic bacterium, Carboxydothermus hydrogenoformans, was identified and overexpressed in Escherichia coli. The enzyme was thermally stable and displayed the highest activity at 70 °C and pH 6.0. It preferred NAD(H) over NADP(H) as a cofactor and ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-1082-5
更新日期:2013-03-01 00:00:00
abstract::Laccases play an important role in the biological break down of lignin and have great potential in the deconstruction of lignocellulosic feedstocks. We examined 16 laccases, both commercially prepared and crude extracts, for their ability to oxidize veratryl alcohol in the presence of various solvents and mediators. S...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-1078-1
更新日期:2013-02-01 00:00:00
abstract::The hybridoma cell line, HB-8696, produces a monoclonal antibody, 520C9 (mouse IgG(1)) that recognizes the breast cancer oncoprotein, c-erbB2. The effect of perfusion rate (volume of fresh feed/working volume of reactor/day) on cell growth and mAb production was investigated but perfusion at a constant rate and at an ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-1058-5
更新日期:2013-02-01 00:00:00
abstract::Seventeen independent transgenic rice plants with the maize anthocyanin regulatory gene Lc under control of the CaMV 35S promoter were obtained and verified by molecular identification. Ten plants showed red spikelets during early development of florets, and the degenerate florets were still red after heading. Additio...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-1046-9
更新日期:2013-01-01 00:00:00
abstract::Microbial solar biofuels offer great promise for future sustainable food, fuels and chemicals but are limited by low productivities and a requirement for large land areas to harvest sunlight. A 71 % increase in combined photosynthetic activity was achieved by illuminating both Rhodobacter sphaeroides and Arthrospira (...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-1021-5
更新日期:2012-12-01 00:00:00
abstract::Arylomycins are type I signal peptidase inhibitors and have a potential as a new type of antibiotics. They were identified from the broth of Streptomyces sp. HCCB10043. The arylomycin biosynthetic gene cluster in this strain was identical to that in S. roseosporus. Within the gene cluster, aryC, encoding a P450 enzyme...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-1032-2
更新日期:2012-12-01 00:00:00
abstract::Blakeslea trispora is used commercially to produce β-carotene. Isopentenyl pyrophosphate isomerase (IPI) and geranylgeranyl pyrophosphate synthase (GGPS) are key enzymes in the biosynthesis of carotenoids. The cDNAs of genes ipi and carG were cloned from the fungus and expressed in Escherichia coli. Greater GGPS activ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-1004-6
更新日期:2012-11-01 00:00:00
abstract::Treatment of a hyperthermophilic enzyme, alkaline phosphatase from Pyrococcus furiosus (PfuAP), with EDTA completely deactivated PfuAP, indicating that the presence of one or more divalent metal ions is essential for its catalytic activity. Subsequent addition of various divalent metal ions to the apoprotein recovered...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0998-0
更新日期:2012-11-01 00:00:00
abstract::The profiles of carotenoids and production of β-carotene by six eustigmatophytes, Eustigmatos magnus, Eustigmatos polyphem, Eustigmatos vischeri, Vischeria helvetica, Vischeria punctata and Vischeria stellata, grown in a bubble column photobioreactor were measured. All eustigmatophytes contained β-carotene, violaxanth...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0996-2
更新日期:2012-11-01 00:00:00
abstract:PURPOSE OF WORK:The biosynthetic pathway of a new antitumor compound, haloroquinone, is elucidated to facilitate metabolic regulation for product accumulation and modification to produce new bioactive structural analogues of the compound. The biosynthetic origin of a novel promising protein kinase B inh...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-1019-z
更新日期:2012-11-01 00:00:00
abstract::ECO-orf27 associated with the cluster of ECO-0501 (LW01) from Amycolatopsis orientalis is deduced to encode a type II thioesterase. Disruption of ECO-orf27 reduced LW01 production by 95 %. Complementation of the disrupted mutant with intact ECO-orf27 restored the production of LW01 suggesting that ECO-orf27 is crucial...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-1010-8
更新日期:2012-11-01 00:00:00
abstract::Pleurotus eryngii was transformed using a polyethylene glycol-mediated method. A plasmid, pEPUGH, containing a reporter gene (enhanced green fluorescent protein gene, egfp) and a positive selectable marker gene (hygromycin phosphotransferase gene, hph) was constructed. The fused egfp-hph gene was placed under the cont...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0985-5
更新日期:2012-10-01 00:00:00