Potential of biodegradable microneedles as a transdermal delivery vehicle for lidocaine.

abstract：:Eugenitin, a chromone derivative and a metabolite of the endophyte Mycoleptodiscus indicus, at 5 mM activated a recombinant GH11 endo-xylanase by 40 %. The in silico prediction of ligand-binding sites on the three-dimensional structure of the endo-xylanase revealed that eugenitin interacts mainly by a hydrogen bond wi...

journal_title：Biotechnology letters

authors： Andrioli WJ,Damásio AR,Silva TM,da Silva VB,Maller A,Nanayakkara NP,Silva CH,Polizeli ML,Bastos JK

更新日期：2012-08-01 00:00:00

abstract：OBJECTIVES:To uncover key genes and pathways regulated by SCL3, a GRAS transcription factor, in the context of gibberellin (GA) in the roots of the model plant Arabidopsis thaliana. RESULTS:Gene expression profiles of ga1-3 mutant and ga1-3 and scl3 double mutant are considerably similar to each other, revealed by Pri...

journal_title：Biotechnology letters

authors： Weng CY,Zhu MH,Liu ZQ,Zheng YG

更新日期：2020-06-01 00:00:00

abstract：:Crude rapeseed oil and post-refining fatty acids were used as substrates for oxalic acid production by a mutant of Aspergillus niger. Both the final concentration and the yield of the product were highest at pH 4 to 5. With a medium containing 50 g lipids l(-1), production reached a maximum of 68 g oxalic acid l(-1) a...

journal_title：Biotechnology letters

authors： Rymowicz W,Lenart D

更新日期：2003-06-01 00:00:00

abstract：:Tetracycline-inducible gene expression (Tet-on) system has become one of the first choices for the control of transgenes expression in mammal and drosophila. However, the Tet-on systems that have been established in mammalian system or tuned into drosophila do not function in the silkworm, Bombyx mori. To construct a ...

journal_title：Biotechnology letters

authors： Karasaki N,Mon H,Takahashi M,Lee JM,Koga K,Kawaguchi Y,Kusakabe T

更新日期：2009-04-01 00:00:00

abstract：OBJECTIVE:To construct a highly expressed and active MEK1R4F (a constitutively active form of mitogen-activated protein kinase kinase 1) by fusion of soluble adenylate kinase (Adk) tag, resulting in Adk-MEK1R4F protein suitable for preparation of phosphorylated ERK. RESULTS:We fused the Adk to the N-terminus of MEK1R4...

journal_title：Biotechnology letters

authors： Yang F,Li H

更新日期：2017-10-01 00:00:00

abstract：:A gene-specific, metagenomic PCR method has led to the discovery of a novel esterase subfamily consisting of five homologous members. Sequence analysis of this esterase subfamily, named the ArmEst subfamily, revealed a unique conserved pattern with a significant variable interior sequence flanked by two symmetric and ...

journal_title：Biotechnology letters

authors： Zhang A,Zhao R,Jin P,Ma L,Xiong X,Xie T,Pei X,Yu L,Yin X,Wang Q

更新日期：2013-11-01 00:00:00

abstract：OBJECTIVES:To engineer the yeast Saccharomyces cerevisiae for the heterologous production of linalool. RESULTS:Expression of linalool synthase gene from Lavandula angustifolia enabled heterologous production of linalool in S. cerevisiae. Downregulation of ERG9 gene, that encodes squalene synthase, by replacing its nat...

journal_title：Biotechnology letters

authors： Amiri P,Shahpiri A,Asadollahi MA,Momenbeik F,Partow S

更新日期：2016-03-01 00:00:00

abstract：:Synthesis of long DNA fragments is often associated with mutations and requires multiple DNA manipulation steps. A novel DNA synthesis method, referred to as patch oligodeoxynucleotide synthesis (POS) to assembly long DNA fragments is presented here. This method involves connection of two types of oligodeoxynucleotide...

journal_title：Biotechnology letters

authors： Yang G,Wang S,Wei H,Ping J,Liu J,Xu L,Zhang W

更新日期：2012-04-01 00:00:00

abstract：:Phanerochaete chrysoporium morphology and manganese peroxidase (MnP) productivity was characterised in a scalable, modularised 1145 cm(3) membrane gradostat reactor in response to switching between an enhanced production medium and a nutrient limited feed (50% C and N reduction). Irrespective of the feed composition u...

journal_title：Biotechnology letters

authors： Govender S

更新日期：2011-02-01 00:00:00

abstract：:Ultraviolet-B (UV-B) radiation has significantly negative effect on cell survival rate (P < 0.01) and positive effect on astaxanthin accumulation (P < 0.01) of Haematococcus pluvialis. H. pluvialis accumulated 3.2 mg/g of astaxanthin when being exposed to 5 W/m(2) of UV-B for 60 min prior to 72 h of high light treatme...

journal_title：Biotechnology letters

authors： Wu Z,Chen G,Chong S,Mak NK,Chen F,Jiang Y

更新日期：2010-12-01 00:00:00

abstract：:Previously, we constructed a genetically modified luciferase of Photinus pyralis that generates more than 10-fold higher luminescence intensity than the wild-type enzyme. In this study, we demonstrate that this modified luciferase enables us to detect ATP at 10(-18) mol, which is almost equal to the quantity contained...

journal_title：Biotechnology letters

authors： Noda K,Matsuno T,Fujii H,Kogure T,Urata M,Asami Y,Kuroda A

更新日期：2008-06-01 00:00:00

abstract：OBJECTIVES:To investigated the potential of a novel dendrosomal nanoformulation of curcumin (DNC) in blocking radiation-induced changes in irradiated human umbilical vein endothelial cells (HUVECs), and their adhesion to human THP-1 monocytoid cells. RESULTS:Co60 gamma rays reduced viability, raised the expression of ...

journal_title：Biotechnology letters

authors： Soltani B,Bodaghabadi N,Mahpour G,Ghaemi N,Sadeghizadeh M

更新日期：2016-12-01 00:00:00

abstract：:Sodium butyrate is commonly used in mammalian cell cultures to increase the productivity of recombinant proteins. A Chinese hamster ovary (CHO) cell line producing recombinant human erythropoietin (rhEPO) was cultured in commercial medium. Addition of 0.5 mM butyrate inhibited the over-growth of the cells after the me...

journal_title：Biotechnology letters

authors： Liu Y,Zhou X,Song Z,Zhang Y

更新日期：2014-05-01 00:00:00

abstract：:A loop-mediated isothermal amplification (LAMP) assay system was employed for detecting Bacillus anthracis spores in pure cultures as well as in various simulated powder samples. The specificity of the designed LAMP primer sets was validated by assaying 13 B. anthracis strains and 33 non-B. anthracis species. The dete...

journal_title：Biotechnology letters

authors： Qiao YM,Guo YC,Zhang XE,Zhou YF,Zhang ZP,Wei HP,Yang RF,Wang DB

更新日期：2007-12-01 00:00:00

abstract：OBJECTIVES:To investigate the effects of operational process conditions on expression of MHC class II protein from a stable Drosophila S2 cell line. RESULTS:When the Drosophila S2 cells were grown in vented orbitally shaken TubeSpin bioreactor 600 containers, cell growth was improved three-fold and the yield of recomb...

journal_title：Biotechnology letters

authors： Shen X,Dojcinovic D,Baldi L,Hacker DL,Luescher IF,Wurm FM

更新日期：2018-01-01 00:00:00

abstract：:Anthocyanic vacuolar inclusions (AVIs) appear as dark red-to-purple spheres of various sizes in vacuoles of grapevine (Vitis vinifera L.) cell suspension culture due to their interaction with anthocyanins. AVIs were purified and the bound anthocyanins extracted and analysed by HPLC from two lines of V. vinifera isolat...

journal_title：Biotechnology letters

authors： Conn S,Zhang W,Franco C

更新日期：2003-06-01 00:00:00

abstract：:A gene, rBmK Cta, encoding a chlorotoxin-like peptide from the scorpion, Buthus martensii Karsch, was synthesized according to the sequence optimized for codon usage in Escherichia coli and was expressed in E. coli BL21 (DE3) using a pExSecI expression system in which the IgG-binding domain-ZZ of protein A is fused to...

journal_title：Biotechnology letters

authors： Fu YJ,Yin LT,Wang W,Chai BF,Liang AH

更新日期：2005-10-01 00:00:00

abstract：:Among single-site mutations of L-arabinose isomerase derived from Geobacillus thermodenitrificans, two mutants were produced having the lowest and highest activities of D-tagatose production. Site-directed mutagenesis at these sites showed that the aromatic ring at amino acid 164 and the size of amino acid 475 were im...

journal_title：Biotechnology letters

authors： Oh HJ,Kim HJ,Oh DK

更新日期：2006-02-01 00:00:00

abstract：:There is a need for a deeper understanding of the biochemical events affecting embryonic stem (ES) cell culture by analyzing the expansion of mouse ES cells in terms of both cell growth and metabolic kinetics. The influence of the initial cell density on cell expansion was assessed. Concomitantly, the biochemical prof...

journal_title：Biotechnology letters

authors： Fernandes TG,Fernandes-Platzgummer AM,da Silva CL,Diogo MM,Cabral JM

更新日期：2010-01-01 00:00:00

abstract：:An endoxylanase gene, xynA, was cloned from Bacillus pumilus ARA and expressed in Escherichia coli. The open reading frame of the xynA gene was 687 bp encoding a signal peptide and a mature xylanase with a molecular mass of 23 kDa. The enzyme was categorized as a glycosyl hydrolase family 11 member based on the sequen...

journal_title：Biotechnology letters

authors： Qu W,Shao W

更新日期：2011-07-01 00:00:00

abstract：OBJECTIVES:A potent HIV vaccine should overcome some limitations such as polymorphism of human HLA, the diversity of HIV-1 virus, and the lack of an effective delivery system. In this study, a DNA construct encoding Nef60-84, Nef126-144, Vpr34-47, Vpr60-75, Gp16030-53, Gp160308-323, and P248-151 epitopes was designed u...

journal_title：Biotechnology letters

authors： Davoodi S,Bolhassani A,Namazi F

更新日期：2021-01-01 00:00:00

abstract：:A new ribonuclease (RNase) with tobacco mosaic virus inhibition was isolated and purified from Bacillus cereus ZH14 through ammonium sulfate precipitation, ultrafiltration, ion-exchange chromatography of DEAE-Sephadex A-50 column, and gel chromatography of Sephacryl S-200HR column. The enzyme was purified approximatel...

journal_title：Biotechnology letters

authors： Zhou WW,Niu TG

更新日期：2009-01-01 00:00:00

abstract：:The rpiB gene, encoding ribose-5-phosphate isomerase (RpiB) from Clostridium thermocellum, was cloned and expressed in Escherichia coli. RpiB converted D-psicose into D-allose but it did not convert D-xylose, L-rhamnose, D-altrose or D-galactose. The production of D-allose by RpiB was maximal at pH 7.5 and 65 degrees ...

journal_title：Biotechnology letters

authors： Park CS,Yeom SJ,Kim HJ,Lee SH,Lee JK,Kim SW,Oh DK

更新日期：2007-09-01 00:00:00

abstract：:The current study aimed to assess the inhibitory effect of a DNA aptamer (Apt17) which targeted Salmonella invasion proteinA (SipA). The effect of Apt17, on biofilm formation by two Salmonella enteritidis strains, was tested either separately or in combination with ampicillin at different Sub MIC concentrations. Maxim...

journal_title：Biotechnology letters

authors： Shatila F,Yaşa İ,Yalçın HT

更新日期：2020-10-01 00:00:00

abstract：:Fed-batch cultivation of Azotobacter vinelandii 21was optimized for biosurfactant production. Optimization of feed-rate time profile and concentration of nutrient medium components in feeding solution is based on a hybrid mathematical model consisting of mass-balance equations for biomass, biosurfactant, volume of cul...

journal_title：Biotechnology letters

authors： Levisauskas D,Galvanauskas V,Zunda G,Grigiskis S

更新日期：2004-07-01 00:00:00

abstract：:Coupling lactate fermentation with cell growth was investigated in shake-flask and bioreactor cultivation systems by increasing aeration to improve lactate productivity in Escherichia coli CICIM B0013-070 (ackA pta pps pflB dld poxB adhE frdA). In shake-flasks, cells reached 1 g dry wt/l then, cultivated at 100 rpm an...

journal_title：Biotechnology letters

authors： Zhou L,Tian KM,Niu DD,Shen W,Shi GY,Singh S,Wang ZX

更新日期：2012-06-01 00:00:00

abstract：:In vitro biocatalysis with cytochrome P450 BM-3 was investigated aiming for the substitution of the expensive natural cofactor NADPH by electrochemistry. The monooxygenase was immobilized on electrodes by entrapment in polypyrrole as a conductive polymer for electrochemically wiring the enzyme. Electropolymerization o...

journal_title：Biotechnology letters

authors： Holtmann D,Mangold KM,Schrader J

更新日期：2009-05-01 00:00:00

abstract：:The enantioselective hydrolysis of insoluble (R,S)-ketoprofen ethyl ester to the optically active (S)-ketoprofen was carried out in a dispersed aqueous lipase reaction system induced by the inclusion of chiral cyclodextrins for complexation of the substrate. Hydroxypropyl-beta-cyclodextrin was the most effective chira...

journal_title：Biotechnology letters

authors： Kim SH,Kim TK,Shin GS,Lee KW,Shin HD,Lee YH

更新日期：2004-06-01 00:00:00

abstract：:An open reading frame (ORF) encoding chitin oligosaccharide deacetylase (Pa-COD) gene and its signal sequence was cloned from the Vibrio parahaemolyticus KN1699 genome and its sequence was analyzed. The ORF encoded a 427 amino acid protein, including the 22 amino acid signal sequence. The deduced amino acid sequence w...

journal_title：Biotechnology letters

authors： Kadokura K,Sakamoto Y,Saito K,Ikegami T,Hirano T,Hakamata W,Oku T,Nishio T

更新日期：2007-08-01 00:00:00

abstract：:Semliki forest virus (SFV) is a pathogen causing lethal encephalitis in laboratory mice. In this study, we obtained three short hairpin RNAs (shRNAs) which could specifically target SFV sequence in GFP reporting systems and effectively suppress SFV replication in luciferase-containing reporter virus system. At a multi...

journal_title：Biotechnology letters

authors： Zhang K,Chen Y,Pan J,Ahola T,Guo D

更新日期：2009-04-01 00:00:00