Abstract:
:Tetracycline-inducible gene expression (Tet-on) system has become one of the first choices for the control of transgenes expression in mammal and drosophila. However, the Tet-on systems that have been established in mammalian system or tuned into drosophila do not function in the silkworm, Bombyx mori. To construct a functional Tet-on system in B. mori, we modified rtTA by introducing a transcription activation domain of immediate-early gene 1 of Autographa californica nuclear polyhedrosis virus and nuclear localization signal of SV40 large T-antigen. The modified rtTA can activate the transcription from 9 x tetO promoter in the silkworm cells up to 250-fold in the presence of doxycycline.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Karasaki N,Mon H,Takahashi M,Lee JM,Koga K,Kawaguchi Y,Kusakabe Tdoi
10.1007/s10529-008-9898-8subject
Has Abstractpub_date
2009-04-01 00:00:00pages
495-500issue
4eissn
0141-5492issn
1573-6776journal_volume
31pub_type
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doi:10.1007/s10529-011-0824-0
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doi:10.1007/s10529-017-2428-9
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2008-10-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2003-11-01 00:00:00