Transgenic tomatoes expressing human beta-amyloid for use as a vaccine against Alzheimer's disease.

Abstract:

:Human beta-amyloid (Abeta) is believed to be one of the main components of Alzheimer's disease, so reduction of Abeta is considered a key therapeutic target. Using Agrobacterium-mediated nuclear transformation, we generated transgenic tomatoes for Abeta with tandem repeats. Integration of the human Abeta gene into the tomato genome and its transcription were detected by PCR and Northern blot, respectively. Expression of the Abeta protein was confirmed by western blot and ELISA, and then the transgenic tomato line expressing the highest protein level was selected for vaccination. Mice immunized orally with total soluble extracts from the transgenic tomato plants elicited an immune response after receiving a booster. The results indicate that tomato plants may provide a useful system for the production of human Abeta antigen.

journal_name

Biotechnol Lett

journal_title

Biotechnology letters

authors

Youm JW,Jeon JH,Kim H,Kim YH,Ko K,Joung H,Kim H

doi

10.1007/s10529-008-9759-5

subject

Has Abstract

pub_date

2008-10-01 00:00:00

pages

1839-45

issue

10

eissn

0141-5492

issn

1573-6776

journal_volume

30

pub_type

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