Abstract:
:Human beta-amyloid (Abeta) is believed to be one of the main components of Alzheimer's disease, so reduction of Abeta is considered a key therapeutic target. Using Agrobacterium-mediated nuclear transformation, we generated transgenic tomatoes for Abeta with tandem repeats. Integration of the human Abeta gene into the tomato genome and its transcription were detected by PCR and Northern blot, respectively. Expression of the Abeta protein was confirmed by western blot and ELISA, and then the transgenic tomato line expressing the highest protein level was selected for vaccination. Mice immunized orally with total soluble extracts from the transgenic tomato plants elicited an immune response after receiving a booster. The results indicate that tomato plants may provide a useful system for the production of human Abeta antigen.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Youm JW,Jeon JH,Kim H,Kim YH,Ko K,Joung H,Kim Hdoi
10.1007/s10529-008-9759-5subject
Has Abstractpub_date
2008-10-01 00:00:00pages
1839-45issue
10eissn
0141-5492issn
1573-6776journal_volume
30pub_type
杂志文章abstract:OBJECTIVES:To investigated the potential of a novel dendrosomal nanoformulation of curcumin (DNC) in blocking radiation-induced changes in irradiated human umbilical vein endothelial cells (HUVECs), and their adhesion to human THP-1 monocytoid cells. RESULTS:Co60 gamma rays reduced viability, raised the expression of ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2189-x
更新日期:2016-12-01 00:00:00
abstract::Microbial fuel cells can be designed to remove nitrogenous compounds out of wastewater, but their performance is at present limited to 0.33 kg NO(3) (-)-Nm(-3) net cathode compartment (NCC) d(-1). By maintaining the pH in the cathode at 7.2, nitrogen removal was increased from 0.22 to 0.50 kg NO(3) (-)-Nm(-3) NCC d(-1...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0048-8
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-0003-x
更新日期:2006-04-01 00:00:00
abstract::Brevibacterium linens forms hydrolytic enzymes which can be used to accelerate the ripening of cheese without causing bitterness. B. linens ATCC 9172 was grown to a high cell density (50 g dry wt l-1 after 60 h) in a mineral medium containing lactic acid, soy-peptone and ammonium sulphate by applying a continuous feed...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1023402529882
更新日期:2003-05-01 00:00:00
abstract::KGLP-1, a 31-amino acid glucagon-like peptide-1 (GLP-1) analogue, has a great therapeutic potential for anti-diabetes. In this work, a strategy for expression and purification of functional KGLP-1 peptide has been established. KGLP-1 cDNA was fused with glutathione S-transferase (GST), with an enterokinase cleavage si...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1526-1
更新日期:2014-08-01 00:00:00
abstract::Sodium butyrate is commonly used in mammalian cell cultures to increase the productivity of recombinant proteins. A Chinese hamster ovary (CHO) cell line producing recombinant human erythropoietin (rhEPO) was cultured in commercial medium. Addition of 0.5 mM butyrate inhibited the over-growth of the cells after the me...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1442-9
更新日期:2014-05-01 00:00:00
abstract::An efficient synthesis of polymerizable 3'- and 5'-O-acyl-nucleoside derivatives has been developed from inosine and 2'-deoxyuridine by enzyme-catalyzed regioselective acylation with divinyl dicarboxylates. In acetone, Lipozyme (immobilized lipase from Mucor miehei) gave 5'-O-acyl-nucleoside products, and PPL (lipase ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000030050.20353.a6
更新日期:2004-06-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1730-z
更新日期:2015-03-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-017-2376-4
更新日期:2017-09-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0883-x
更新日期:2012-06-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-1032-2
更新日期:2012-12-01 00:00:00
abstract::A purified recombinant enzyme from Spirochaeta thermophila, that is suggested to be a cellobiose 2-epimerase, was a 47 kDa monomer with a specific activity of 29.2 U min(-1) for mannobiose. The epimerization activity of the recombinant enzyme for mannobiose was maximal at pH 7.0 and 60 °C with a half-life of 124 h. Th...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1267-6
更新日期:2013-11-01 00:00:00
abstract::Genomics, transcriptomics, proteomics and fluxomics are powerful omics-technologies that play a major role in today's research. For each of these techniques good sample quality is crucial. Major factors contributing to the quality of a sample is the actual sampling procedure itself and the way the sample is stored dir...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1163-0
更新日期:2013-06-01 00:00:00
abstract::Three chloroplast genes, chlL, chlN and chlB, encoding the light-independent protochlorophyllide oxidoreductase (LIPOR) in Chlorella protothecoides CS-41 growing either photoautotrophically, mixotrophically or heterotrophically, were all transcribed constitutively independent of illumination and presence of glucose. S...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-5528-x
更新日期:2006-02-01 00:00:00
abstract:OBJECTIVE:To investigate the aerotolerance of Lactobacillus rhamnosus hsryfm 1301 and its influencing factors. RESULTS:The growth rate of L. rhamnosus hsryfm 1301 weakened noticeably when the concentration of supplemented H2O2 reached 1 mM, and only 2% of all L. rhamnosus hsryfm 1301 cells survived in MRS broth supple...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-018-2523-6
更新日期:2018-04-01 00:00:00
abstract::A brown-rot fungus, Fomitopsis pinicola, degraded polyvinyl alcohol (PVA) in quartz sand but not in liquid culture. From gel permeation chromatography analysis, the high-molecular-weight fraction of PVA was decreased by the action of F. pinicola but the coloration of the culture filtrate with I2 solution increased. Th...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1281-8
更新日期:2013-11-01 00:00:00
abstract::A polysaccharide from the traditional Chinese medicinal herb, Bletilla striata (Thunb.) Reichb. f., was isolated, purified and characterized. It induced the proliferation of human umbilical vascular endothelial cells and the expression of vascular endothelial growth factor up to 156% and 147% of control after 72 h, re...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-0011-x
更新日期:2006-04-01 00:00:00
abstract::O-Acetylserine (thiol) lyase (OASTL, EC 2.5.1.47) catalyzes the final step in cysteine biosynthesis. The soybean GmOASTL4 gene was overexpressed in tobacco. Transgenic plants showed markedly increased accumulation of transcripts and higher cysteine content compared with the wild-type. Upon exposure to cadmium stress, ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0178-z
更新日期:2010-04-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1529-y
更新日期:2014-08-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-015-1907-0
更新日期:2015-11-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0413-7
更新日期:2011-01-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0904-9
更新日期:2012-07-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2338-x
更新日期:2017-07-01 00:00:00
abstract::Alkyl glucosides were synthesized by the reaction of Leuconostoc mesenteroides dextransucrase with sucrose and various alcohols. Alkyl alpha-D-glucosides were obtained with a yield of 30% (mol/mol) with primary alcohols, but secondary alcohols or tertiary alcohols gave yields below 5%. The optimal yield was 50% using ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0015-4
更新日期:2009-09-01 00:00:00
abstract::An automated sequential injection analysis (SIA) system using stop-flow technique was developed to determine methanol concentration by means of the enzymatic reactions of alcohol oxidase and peroxidase. Its application as an on-line device for monitoring Pichia pastoris fermentations producing an heterologous protein ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1026228518418
更新日期:2003-11-01 00:00:00
abstract::Growth of Ruta graveolens shoots was induced when Bacillus sp. cell lysates were added to the culture medium. Elicitation of coumarin by this lysate was also very effective; the concentrations of isopimpinelin, xanthotoxin and bergapten increased to 610, 2120 and 1460 microg g(-1) dry wt, respectively. It also had a s...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9559-3
更新日期:2008-03-01 00:00:00
abstract::Recombinant Mycobacterium sp. strain MR65 carrying dszABCD genes was used for desulfurization of 10-methylbenzo[b]naphtho[2,1-d]thiophene (10-methyl BNT) in the hexadecane phase. The specific activity was 25% of that of dibenzothiophene (DBT). One of two major metabolites of 10-methyl BNT produced by strain MR65 was i...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1023584805911
更新日期:2003-05-01 00:00:00
abstract::An efficient method for creating a DNA library is presented in which gene mutagenesis and recombination can be introduced by integrating error-prone PCR with a staggered extension process in one test tube. In this process, less than 15 cycles of error-prone PCR are used to introduce random mutations. After precipitate...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9674-9
更新日期:2008-07-01 00:00:00
abstract::Cell-based therapeutics are promising routes for the regeneration of damaged cells and organs. The recovery of cells cultured in vitro for such applications requires the use of proteolytic enzymes which deteriorate its property by disruption of cell-cell and cell-matrix interactions. Intact cell sheets can be retrieve...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0652-2
更新日期:2011-10-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-1884-9
更新日期:2005-03-01 00:00:00