Abstract:
OBJECTIVES:To develop a rapid, dual-parameter, plate-based screening process to improve production and secretion rate of glucose oxidase simultaneously in Aspergillus niger. RESULTS:A morphology engineering based on CaCO3 was implemented, where the yield of GOD by A. niger was increased by up to 50%. Analysis of extracellular GOD activity was achieved in 96-well plates. There was a close negative correlation between the total GOD activity and its residual glucose of the fermentation broth. Based on this, a rapid, plate-based, qualitative analysis method of the total GOD activity was developed. Compared with the conventional analysis method using o-dianisidine, a correlation coefficient of -0.92 by statistical analysis was obtained. CONCLUSION:Using this dual-parameter screening method, we acquired a strain with GOD activity of 3126 U l-1, which was 146% higher than the original strain. Its secretion rate of GOD was 83, 32% higher than the original strain.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Zhu X,Sun J,Chu Jdoi
10.1007/s10529-017-2442-ysubject
Has Abstractpub_date
2018-01-01 00:00:00pages
103-110issue
1eissn
0141-5492issn
1573-6776pii
10.1007/s10529-017-2442-yjournal_volume
40pub_type
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