Abstract:
:Two isoforms of laccase produced from the culture supernatant of Pycnoporus sanguineus were partially purified by phenyl-Sepharose chromatography. Molecular masses of the enzymes were 80 kDa (Lac I) and 68 kDa (Lac II). Optimum activity of Lac I was at pH 4.8 and 30 degrees C, and Lac II was at pH 4.2 and 50 degrees C over 5 min reaction. The Km values of enzymes toward syringaldazine were 10 microM: (Lac I) and 8 microM: (Lac II). Sodium azide inhibited Lac I (85%) and Lac II (75%) activities.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Garcia TA,Santiago MF,Ulhoa CJdoi
10.1007/s10529-006-0026-3subject
Has Abstractpub_date
2006-05-01 00:00:00pages
633-6issue
9eissn
0141-5492issn
1573-6776journal_volume
28pub_type
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