Abstract:
:A fully contained and efficient heterologous protein production system was designed using Brassica rapa rapa (turnip) hairy roots. Two expression cassettes containing a cauliflower mosaic virus (CaMV) 35S promoter with a duplicated enhancer region, an Arabidopsis thaliana sequence encoding a signal peptide and the CaMV polyadenylation signal were constructed. One cassette was used to express the green fluorescent protein (GFP)-encoding gene in hairy roots grown in flasks. A stable and fast-growing hairy root line secreted GFP at >120 mg/l culture medium. GFP represented 60 % of the total soluble proteins in the culture medium. Turnip hairy roots retained sustainable growth and stable GFP production over 3 years. These results were superior to those obtained using tobacco hairy roots.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Huet Y,Ekouna JP,Caron A,Mezreb K,Boitel-Conti M,Guerineau Fdoi
10.1007/s10529-013-1335-ysubject
Has Abstractpub_date
2014-01-01 00:00:00pages
181-90issue
1eissn
0141-5492issn
1573-6776journal_volume
36pub_type
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