Abstract:
OBJECTIVE:To investigate the role of cytosine methylation in regulation of polyketide compounds biosynthesis in larvae of Strongylocentrotus intermedius. RESULTS:Treatment of S. intermedius larvae with 100 and 200 µM 5-azacytidine (5A) as a DNA demethylating agent significantly increased the amounts of spinochrome D and spinochrome E, as the number of pigmented cells per studied larva, in a dose-depended manner. The data on SiPks gene expression showed enhancement in 16- and 67-fold in S. intermedius larvae treated with 100 and 200 µM 5A, respectively, relative to untreated ones. Moreover, the activation of transcription factors SiGcm, SiGatae and SiKrl gene expression involved in regulation of SiPks was observed in S. intermedius larvae upon treatment with 5A, suggesting DNA methylation being powerful regulator of polyketide compounds biosynthesis. CONCLUSIONS:This is the first study to describe the role of cytosine DNA methylation in the regulation of polyketide compounds biosynthesis in sea urchins. Current study implies a negative control provided by cytosine DNA methylation machinery as a key regulator of polyketide compound biosynthesis.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Tyunin AP,Ageenko NV,Kiselev KVdoi
10.1007/s10529-016-2191-3subject
Has Abstractpub_date
2016-12-01 00:00:00pages
2035-2041issue
12eissn
0141-5492issn
1573-6776pii
10.1007/s10529-016-2191-3journal_volume
38pub_type
杂志文章abstract::Human beta-amyloid (Abeta) is believed to be one of the main components of Alzheimer's disease, so reduction of Abeta is considered a key therapeutic target. Using Agrobacterium-mediated nuclear transformation, we generated transgenic tomatoes for Abeta with tandem repeats. Integration of the human Abeta gene into the...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9759-5
更新日期:2008-10-01 00:00:00
abstract::At the core of a biomanufacturing process for recombinant proteins is the production cell line. It influences the productivity and product quality. Its characteristics also dictate process development, as the process is optimized to complement the producing cell to achieve the target productivity and quality. Advances...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-015-1843-z
更新日期:2015-08-01 00:00:00
abstract:OBJECTIVES:To screen the phylogenetically-nearest members of Cellulosimicrobium cellulans for the production of cellulosome-like multienzyme complexes and extracellular β-xylosidase activity against 7-xylosyltaxanes and to get corresponding molecular insights. RESULTS:Cellulosimicrobium (family Promicromonosporaceae) ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2469-0
更新日期:2018-02-01 00:00:00
abstract:OBJECTIVES:To investigate the effects of operational process conditions on expression of MHC class II protein from a stable Drosophila S2 cell line. RESULTS:When the Drosophila S2 cells were grown in vented orbitally shaken TubeSpin bioreactor 600 containers, cell growth was improved three-fold and the yield of recomb...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2440-0
更新日期:2018-01-01 00:00:00
abstract:OBJECTIVES:To investigate gene expression profiles of the thermotolerant yeast Saccharomyces cerevisiae strain KKU-VN8, a potential high-ethanol producer, in response to various stresses during high-temperature ethanol fermentation using sweet sorghum juice (SSJ) under optimal conditions. RESULTS:The maximal ethanol c...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2398-y
更新日期:2017-10-01 00:00:00
abstract::Oligo-chitosans were chemically modified with dinitrophenyl groups for selective and rapid adsorption of amitriptyline by forming pi-pi complexes. 1H-NMR was utilized not only for characterization of modified chitosans but also for monitoring the aromatic-aromatic interaction. The variation in the chemical shift of ar...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000024094.16474.35
更新日期:2004-05-01 00:00:00
abstract::Microbial solar biofuels offer great promise for future sustainable food, fuels and chemicals but are limited by low productivities and a requirement for large land areas to harvest sunlight. A 71 % increase in combined photosynthetic activity was achieved by illuminating both Rhodobacter sphaeroides and Arthrospira (...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-1021-5
更新日期:2012-12-01 00:00:00
abstract:OBJECTIVES:The action modes of an endocellulase, EGA, and its domains (CD9 and CBM3) during enzymatic treatment of cotton fabrics were investigated. RESULTS:EGA, CD9 and CBM3 had the binding capacity to cellulose substrates, of which the filter paper was the substrate with the strongest binding capacity. Analyses of s...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1832-2
更新日期:2015-08-01 00:00:00
abstract::miR-21 is highly expressed in a variety of cancers, suggesting that it might play a role in the process of oncogenesis, as supported by it directly causing pre-B cell lymphomas in transgenic mice. Rm21LG transgenic mice for the conditional co-expression of miR-21 and luciferase (Luc) mediated by Cre/lox P system were ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1327-y
更新日期:2014-01-01 00:00:00
abstract::Aptamers against inactive Vibrio alginolyticus were selected from an 82-nt ssDNA random library by systematic evolution of ligands by exponential enrichment. After 15 rounds of selection, the final pool of aptamers was highly specific for inactivated V. alginolyticus and had a dissociation constant of 27.5 ± 9.2 nM. U...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1154-1
更新日期:2013-06-01 00:00:00
abstract::Lipase-catalyzed synthesis of potential multifunctional ribavirin derivatives was performed in acetone. Divinyl dicarboxylates with different chain lengths (C4, C6, C9, C10) were used as acyl donors and the reactions were catalyzed by lipase immobilized on acrylic resin from Candida antarctica (CAL-B). Ribavirin was r...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-5188-x
更新日期:2005-05-01 00:00:00
abstract:OBJECTIVE:The effects of a brief (3 days) and prolonged (6 days) period of incubation in darkness and light on the biomass content, lipid content and fatty acid profile in Chlorella vulgaris UMT-M1 were determined. RESULTS:Three days of incubation in darkness increased saturated fatty acid (SFA) content from 34.0 to 4...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-021-03077-2
更新日期:2021-01-12 00:00:00
abstract::The astaxanthin biosynthesis gene cluster in Paracoccus haeundaensis consists of six genes: crtW, crtZ, crtY, crtI, crtB, and crtE contain 726, 486, 1158, 1503, 912, and 879 base pairs, respectively. Individual carotenoid biosynthesis genes of P. haeundaensis have now been expressed in E. coli and each gene product ha...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9072-0
更新日期:2006-08-01 00:00:00
abstract::Methods for the preparation of carrier-free insoluble enzymes are reviewed. The technology of cross-linked enzyme aggregates has now been applied to a range of synthetically useful activities. Fusion proteins are also gaining momentum because they allow a relatively selective aggregation or even a specific self-assemb...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-009-0173-4
更新日期:2010-03-01 00:00:00
abstract::The exploded bast, branch and stem of cotton stalk were degraded by alkalophilic Bacillus NT-19, with weight losses of 24%, 20% and 14%, respectively, after 14 d. Compared with a white-rot fungus (Phanerochaete chrysosporium), Bacillus NT- 19 preferentially degraded the non-cellulose components of cotton stem. The rel...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1026083827968
更新日期:2003-10-01 00:00:00
abstract::Constant medium feeding rate and intermittent fed-batch fermentation strategies were investigated aiming to increase the yields of γ-decalactone production by Yarrowia lipolytica, using methyl ricinoleate as substrate and ricinoleic acid source. The accumulation of another compound, 3-hydroxy-γ-decalactone, was also a...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0824-0
更新日期:2012-04-01 00:00:00
abstract:OBJECTIVE:Myelin oligodendrocyte glycoprotein (MOG) is one of the major autoantigens in multiple sclerosis (MS), therefore selective depletion of autoreactive lymphocytes exposing MOG-specific B cell receptors (BCRs) would be beneficial in terms of MS treatment. RESULTS:Using E. coli we generated an efficient protocol...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2092-5
更新日期:2016-07-01 00:00:00
abstract::Some pest management programs employ PCR to identify cry1 genes from Bacillus thuringiensis to predict bacterial toxicity towards different insect pests. However, due to changes on the mode of action of the Cry proteins, new primers had to be designed to detect the new genes. Therefore, an 'in-silico' study of genetic...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0088-0
更新日期:2009-12-01 00:00:00
abstract::An integrative vector was constructed for inserting heterologous genes within a non-functional open reading frame (ORF) on the chromosome of Streptococcus thermophilus. The vector, pINTRS, contained a temperature sensitive origin of replication and an erythromycin resistance gene for initial selection in S. thermophil...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-9931-6
更新日期:2009-05-01 00:00:00
abstract::Many enzymes used as digestive aids exhibit, at best, moderate stability when incubated under gastrointestinal conditions. A supplemental β-galactosidase administered orally to treat lactose intolerance was conjugated to 40 kDa, branched polyethylene glycol (PEG). PEGylation increased the enzyme's relative activity at...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0474-7
更新日期:2011-03-01 00:00:00
abstract::An efficient method for creating a DNA library is presented in which gene mutagenesis and recombination can be introduced by integrating error-prone PCR with a staggered extension process in one test tube. In this process, less than 15 cycles of error-prone PCR are used to introduce random mutations. After precipitate...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9674-9
更新日期:2008-07-01 00:00:00
abstract:OBJECTIVES:Exogenous phytase improved the activity of hydrolases to decrease the malting time. RESULTS:Treatment with phytase during barley steeping increased activity of hydrolases (α-/β-amylase, proteinase, β-glucanase and xylanase) in green malt. Maximal activity was observed for α-/β-amylase, β-glucanase and xylan...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2394-2
更新日期:2017-12-01 00:00:00
abstract::Microbial desulfurization is potentially an alternative process to chemical desulfurization of fossil fuels and their refined products. The dibenzothiophene desulfurizing system of Rhodococcus erythropolis includes DszD which is an NADH-dependent FMN oxidoreductase with 192 residues that is responsible for supplying r...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0254-4
更新日期:2010-07-01 00:00:00
abstract::The role of transglutaminase II (TGase II) in hyaluronic acid (HA)-promoted melanoma cell motility was investigated. HA induced the expression of TGase II via the nuclear factor kappaB (NF-kB) in melanoma cells. HA increased the Rac1 activity and phosphorylation of focal adhesion kinase (FAK). Transfection by lipofect...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9496-1
更新日期:2008-01-01 00:00:00
abstract:OBJECTIVE:To characterize the hydrolysis product and the substrate binding in the catalytic cavity of α-agarase AgaD. RESULTS:The time course curve showed that AgaD degraded agarose by the endo-type cleavage. AgaD did not degrade agarobiose (A2) and agarotetraose (A4). The minimum-length substrate was agarohexaose (A6...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-020-02901-5
更新日期:2020-10-01 00:00:00
abstract::The transfer of the IncP-7 carbazole degradative plasmid pCAR1 from Pseudomonas putida SM1443 (derived from strain KT2440) into bacteria of river water samples was monitored using a reporter gene encoding red fluorescent protein (RFP). The number of transconjugants drastically increased in the presence of carbazole, a...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9519-y
更新日期:2008-01-01 00:00:00
abstract::We truncated the VP2 protein of infectious bursal disease virus into five fragments: V1-5. All fragments were displayed on the inner membrane of the Escherichia coli periplasm. After disruption of the outer membrane, spheroplasts that had anchored with the VP2 fragment were incubated with an anti-VP2 polyclonal antibo...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1400-6
更新日期:2014-03-01 00:00:00
abstract::A recombinant allophycocyanin (rAPC), used for treatment of tumors, has been expressed in E. coli which was grown in glucose fed-batch culture in a 30 l fermentor. Recombinant allophycocyanin was purified from soluble E. coli cell lysate using hydrophobic interaction chromatography followed by chromatography using amy...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-5794-7
更新日期:2005-06-01 00:00:00
abstract::A sigma 70-like gene, sigA, has been identified from Corynebacterium ammoniagenes. The sigA gene encodes a polypeptide of 467 amino acids with a calculated molecular mass of 52036 Da. The deduced amino acid sequence preserves the common motifs of the primary sigma factors and shows very high similarity to those of Sig...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000021962.16204.08
更新日期:2004-04-01 00:00:00
abstract::A recombinant baculovirus was constructed to simultaneously express codon-optimized virus-like particles (VLP), A VP1-2A-VP3 and VP0 of serotype O foot-and-mouth disease virus (FMDV), from individual promoters. The target proteins were expressed in insect cells at high level, as shown by indirect sandwich ELISA; and t...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0295-8
更新日期:2010-09-01 00:00:00