Abstract:
:Methods for the preparation of carrier-free insoluble enzymes are reviewed. The technology of cross-linked enzyme aggregates has now been applied to a range of synthetically useful activities. Fusion proteins are also gaining momentum because they allow a relatively selective aggregation or even a specific self-assembly of the desired enzyme activity into insoluble particles in the absence of potentially denaturing chemicals required for precipitation and cross-linking. Recycling of insoluble protein particles for multiple rounds of batchwise reaction has been demonstrated in selected biotransformations. However, for application in a fully continuous biocatalytic process, low resistance to mechanical stress and high compressibility are issues for consideration on carrier-free enzyme particles.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Roessl U,Nahálka J,Nidetzky Bdoi
10.1007/s10529-009-0173-4subject
Has Abstractpub_date
2010-03-01 00:00:00pages
341-50issue
3eissn
0141-5492issn
1573-6776journal_volume
32pub_type
杂志文章,评审abstract::Cell division of the wild type strain Corynebacterium (formerly Brevibacterium) ammoniagenes ATCC 6872 which requires 1 microM Mn2+ for balanced growth was inhibited by addition of 20 mM hydroxyurea (HU) or 10 mM p-methoxyphenol (MP) to a Mn2+-supplemented fermentation medium at an appropriate time. Scanning electron ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1021919404066
更新日期:2003-01-01 00:00:00
abstract::Oligo-chitosans were chemically modified with dinitrophenyl groups for selective and rapid adsorption of amitriptyline by forming pi-pi complexes. 1H-NMR was utilized not only for characterization of modified chitosans but also for monitoring the aromatic-aromatic interaction. The variation in the chemical shift of ar...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000024094.16474.35
更新日期:2004-05-01 00:00:00
abstract:OBJECTIVES:To rationally identify targets for enhancing adenosine production, transcription level of genes involved in adenosine synthesis of Bacillus subtilis XGL was detected during the fermentation process, complemented with metabolite pool analysis. RESULTS:PurR-regulated genes (pur operon and purA) and prs were d...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1801-9
更新日期:2015-07-01 00:00:00
abstract::Heterologous expression in Escherichia coli often leads to production of the expressed proteins as insoluble and inactive inclusion bodies. The general strategy for protein recovery includes isolation and washing of inclusion bodies, solubilization of aggregated protein and refolding of solubilized protein. The proces...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-0024-x
更新日期:2005-08-01 00:00:00
abstract:OBJECTIVE:To engineer Pichia pastoris for heterologous production of cellulase from Musca domestica and explore its potential for industrial applications. RESULTS:A new beta-glucosidase gene (bg), encoding 562 amino acids, was cloned from M. domestica by using rapid amplification of cDNA ends. The gene bg was linked t...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2351-0
更新日期:2017-08-01 00:00:00
abstract::Electrotransformation of Thermoanaerobacter ethanolicus JW200 was achieved using the plasmid, pTE16, and a pUC-based suicide vector, pTEA2. The construct pTE16 is based on the Escherichia coli-Clostridium perfringens shuttle vector pJIR715 and contains a thermostable chloramphenicol (Cm) resistance cassette. Evidence ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9184-6
更新日期:2006-12-01 00:00:00
abstract::Solubilization of lignin and carbohydrates from the lignin-holocellulose structure of cotton seed-coat fragments was investigated by UV/VIS spectrometry. Xylanase (Pulpzyme HC) pre-treatment partially destroyed the lignocellulosic structure of the seed-coat fragments, producing reducing sugars and soluble lignin in th...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9042-6
更新日期:2006-05-01 00:00:00
abstract::Four Cr(VI)-reducing bacterial strains (Ochrobactrum intermedium, CrT-2, CrT-3 and CrT-4) previously isolated from chromium-contaminated sites were inoculated on to seeds of sunflower (Helianthus annuus var SF-187), which were germinated and grown along with non-inoculated controls with chromate salts (300 microg CrCl...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-7188-2
更新日期:2005-07-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2371-9
更新日期:2017-09-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2469-0
更新日期:2018-02-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1670-7
更新日期:2015-01-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1631-1
更新日期:2014-12-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-9920-9
更新日期:2009-05-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9636-2
更新日期:2008-05-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9039-1
更新日期:2006-07-01 00:00:00
abstract::Human DNA polymerase iota (Pol ι) is a Y-family DNA polymerase with unusual biochemical properties and not fully understood functions. Pol ι preferentially incorporates dGTP opposite template thymine. This property can be used to monitor Pol ι activity in the presence of other DNA polymerases, e.g. in cell extracts of...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0764-8
更新日期:2012-02-01 00:00:00
abstract::The basic reactions of the clostridial 1-butanol biosynthesis pathway can be regarded to be the inverted reactions of the fatty acid β-oxidation pathway. A pathway for the biosynthesis of fuels and chemicals was recently engineered by combining enzymes from both aerobic and anaerobic fatty acid β-oxidation as well as ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0797-z
更新日期:2012-03-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1026038201193
更新日期:2003-10-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0292-y
更新日期:2010-09-01 00:00:00
abstract::Mucor circinelloides and Mortierella isabellina hydroxylated dehydroabietic acid (DehA). DehA was converted regio- and stereo-selectively by whole cells of Mr. circinelloides to give 2alpha-hydroxydehydroabietic acid in a 75% molar conversion yield (11 mM from 14.7 mM DehA) after 72 h in the cultivation medium contain...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-3224-5
更新日期:2005-09-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9105-8
更新日期:2006-09-01 00:00:00
abstract::The inhibition of protein-tyrosine phosphatase 1B (PTP1B) is a potential target for treatment of type 2 diabetes. Vanadium and zinc metal coordinated complexes have insulin-enhancing activities, and while vanadium compounds inhibit PTP1B, little is known on the mode of action of zinc compounds. In this study we develo...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-004-7855-8
更新日期:2005-02-01 00:00:00
abstract:OBJECTIVE:Heterotrophic manganese (Mn)-oxidizing microorganisms responsible for biogenic manganese oxide (Bio-MnOx) production are fastidious. Their enrichment is not easily accomplished by merely adding a soluble organic substrate to non-sterile mixed cultures. The objective of this study was to evaluate polycaprolact...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-021-03088-z
更新日期:2021-01-26 00:00:00
abstract:OBJECTIVE:In this study, we aimed to maximize glutathione (GSH) production by a metabolically engineered Yarrowia lipolytica strain using a small-scale optimization approach. RESULTS:A three levels four factorial Box-Behnken Design was used to assess the effect of pH, inulin extract, yeast extract and ammonium sulfate...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-03039-0
更新日期:2021-02-01 00:00:00
abstract::Seeds play essential roles in plant life cycle and germination is a complex process which is associated with different phases of water imbibition. Upon imbibition, seeds begin utilization of storage substances coupled with metabolic activity and biosynthesis of new proteins. Regeneration of organelles and emergence of...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-017-2393-3
更新日期:2017-10-01 00:00:00
abstract::MicroRNA (miRNA) profiling is a first important step in elucidating miRNA functions. Real time quantitative PCR (RT-qPCR) and microarray hybridization approaches as well as ultra high throughput sequencing of miRNAs (small RNA-seq) are popular and widely used profiling methods. All of these profiling approaches face s...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0380-z
更新日期:2010-12-01 00:00:00
abstract:PURPOSE OF WORK:To increase the bioproduction of L-phenylacetylcarbinol (PAC), a precursor molecule in the synthesis of the decongestants ephedrine and pseudoephedrine and which suffers from substrate, product, and by-product inhibition, by ensuring that the delivery of the substrate, benzaldehyde, is maintained within...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0408-4
更新日期:2011-01-01 00:00:00
abstract::The limiting step for biosynthesis of 3-hydroxypropionic acid (3-HP) in Klebsiella pneumoniae is the conversion of 3-hydroxypropionaldehyde (3-HPA) to 3-HP. This reaction is catalyzed by aldehyde dehydrogenase (ALDH) with NAD(+) as a cofactor. Although NAD(+)-dependent ALDH overexpression facilitates 3-HP biosynthesis...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1590-6
更新日期:2014-11-01 00:00:00
abstract::Tandem affinity purification (TAP) is a methodology for the isolation of protein complexes from endogenous sources. It involves incorporation of a dual-affinity tag into the protein of interest and introduction of the construct into desired cell lines or organisms. Using the two affinity handles, the protein complex a...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-011-0592-x
更新日期:2011-08-01 00:00:00
abstract:OBJECTIVES:To identify and characterize a novel antimicrobial peptide, catesbeianin-1. RESULTS:Catesbeianin-1 is 25 amino acids long and is α-helical, cationic and amphipathic. It had antimicrobial activity against Gram-positive and Gram-negative bacteria. It was resistant against trypsin and pepsin. Catesbeianin-1 ex...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2312-7
更新日期:2017-06-01 00:00:00