Applicability of Coriolopsis rigida for biodegradation of polycyclic aromatic hydrocarbons.

abstract：OBJECTIVES:To design and construct a novel bio-anode electrode based on the oxidation of glutamic acid to produce 2-oxoglutarate, generating two electrons from NADH. RESULTS:Efficient enzyme reaction and electron transfer were observed owing to immobilization of the two enzymes using a mixed self-assembled monolayer. ...

journal_title：Biotechnology letters

authors： Sakamoto H,Komatsu T,Yamasaki K,Satomura T,Suye SI

更新日期：2017-02-01 00:00:00

abstract：OBJECTIVES:To engineer the yeast Saccharomyces cerevisiae for the heterologous production of linalool. RESULTS:Expression of linalool synthase gene from Lavandula angustifolia enabled heterologous production of linalool in S. cerevisiae. Downregulation of ERG9 gene, that encodes squalene synthase, by replacing its nat...

journal_title：Biotechnology letters

authors： Amiri P,Shahpiri A,Asadollahi MA,Momenbeik F,Partow S

更新日期：2016-03-01 00:00:00

abstract：:Fermentation kinetics of Penicillium aculeatum ATCC 10409 demonstrated that fungal growth and dextranase release are decoupled. Inoculation by conidia or mycelia resulted in identical kinetics. Two new isoenzymes of the dextranase were characterized regarding their kinetic constants, pI, MW, activation energy and stab...

journal_title：Biotechnology letters

authors： Erhardt FA,Stammen S,Jördening HJ

更新日期：2008-06-01 00:00:00

abstract：:Streptomyces is an attractive host for heterologous protein secretion. To further optimize its expression capacity, better expression vectors will be helpful. Here, based on pSGL1, a high copy number plasmid present in Streptomyces globisporus C-1027, we constructed a series of novel E. coli-Streptomyces shuttle expre...

journal_title：Biotechnology letters

authors： Zhu Y,Wang L,Du Y,Wang S,Yu T,Hong B

更新日期：2011-02-01 00:00:00

abstract：PURPOSE OF WORK:The purpose of this study is to report a ι-carrageenase which degrades ι-carrageenan yielding neo-ι-carratetraose as the main product in the absence of NaCl. The gene for a new ι-carrageenase, CgiB_Ce, from Cellulophaga sp. QY3 was cloned and sequenced. It comprised an ORF of 1,386 bp encoding for a pro...

journal_title：Biotechnology letters

authors： Ma S,Tan YL,Yu WG,Han F

更新日期：2013-10-01 00:00:00

abstract：:A microtiter plate-based assay was developed to evaluate the ability of lipases to perform transesterifications when employed in different organic solvents. A 4-nitrophenol assay was carried out employing seven different lipase formulations and two fatty acid methyl esters with different chain lengths in a total of si...

journal_title：Biotechnology letters

authors： Pöhnlein M,Finkbeiner T,Syldatk C,Hausmann R

更新日期：2015-03-01 00:00:00

abstract：:Classical purification of the glycoprotein equine chorionic gonadotropin (eCG) from serum includes pH fractionation with metaphosphoric acid, two ethanol precipitation steps as well as dialysis followed by fixed-bed chromatography. A simplified process requiring only 1/3 of the solvent and improving the yield from 53 ...

journal_title：Biotechnology letters

authors： Müller C,Wagner K,Frankenfeld K,Franzreb M

更新日期：2011-05-01 00:00:00

abstract：:The novel cancer/testis antigen gene, NY-SAR-35, is expressed exclusively in normal testis and in various histological types of tumor. However, the NY-SAR-35 gene expression is observed to be aberrant in several cancer cell lines and tissues. The analysis of methylation status of the NY-SAR-35 gene promoter in various...

journal_title：Biotechnology letters

authors： Park JH,Song MH,Lee CH,Lee MK,Park YM,Old L,Lee SY

更新日期：2011-06-01 00:00:00

abstract：OBJECTIVE:To evaluate the therapeutic potential of human umbilical cord blood mesenchymal stem cells (hUCBMSCs) on promoting erectile function in a rat model of bilateral cavernous nerve (CN) crush injury. RESULTS:Fifty male Sprague-Dawley rats were randomly assigned to sham + PBS group (n = 10), BCNI (bilateral caver...

journal_title：Biotechnology letters

authors： Zhu JQ,Lu HK,Cui ZQ,Wang YC,Li YH,Zhao W,Fu Q,Xu YM,Xu Y,Song LJ

更新日期：2015-07-01 00:00:00

abstract：:Peroxisome proliferator-activated receptors (PPARs) alpha and gamma are key regulators of lipid homeostasis and insulin resistance. In this study we show that a novel compound, 3-{4-[2-(5-methyl-2-phenyl-oxazol-4-yl)-ethoxy]-phenyl}- 2-[2-(2-nitro-phenoxy)-acetyl amino]-propionic acid (O325H), is an agonist with dual ...

journal_title：Biotechnology letters

authors： Xu C,Wang LL,Liu HY,Ruan CM,Zhou XB,Cao YL,Li S

更新日期：2006-06-01 00:00:00

abstract：:Cellobiose is a major intermediate from cellulase hydrolysis of pretreated plant biomass. Engineering biocatalysts for direct use of cellobiose could eliminate the need for exogenous β-glucosidase. Additionally, rapid removal of cellobiose in a simultaneous saccharification and fermentation facilitates enzymatic hydro...

journal_title：Biotechnology letters

authors： Rutter C,Chen R

更新日期：2014-02-01 00:00:00

abstract：:The protective effects of polymer additives, including a group of viscosity-enhancing polymer poly-γ-glutamic acid (γPGA; 10, 50, and 500 kDa) and surface-active polymer Pluronic F68, on Chinese hamster ovary cells against damage due to shear stress were investigated in shake-flask cultures. The level of protection wa...

journal_title：Biotechnology letters

authors： Chun BH,Lee YK,Chung N

更新日期：2012-10-01 00:00:00

abstract：OBJECTIVE:To engineer Pichia pastoris for heterologous production of cellulase from Musca domestica and explore its potential for industrial applications. RESULTS:A new beta-glucosidase gene (bg), encoding 562 amino acids, was cloned from M. domestica by using rapid amplification of cDNA ends. The gene bg was linked t...

journal_title：Biotechnology letters

authors： Zhang S,Huang J,Hu R,Guo G,Shang X,Wu J

更新日期：2017-08-01 00:00:00

abstract：:An acoustic cell settler (ACS) using ultrasound at cells of 3 MHz was used to recycle Saccharomyces cerevisiae in a fermenter. The locations of both the inlet and outlet in the acoustic cell settler, which have a relatively long distance between the transducer and reflector, were optimized. A tilted settler was design...

journal_title：Biotechnology letters

authors： Hwang SH,Koo YM

更新日期：2003-02-01 00:00:00

abstract：OBJECTIVES:To study the roles and mechanisms of HuR in cancer stem cell maintenance of lung cancer. RESULTS:HuR expression was increased in tumor spheres of lung cancer cells. Knockdown of HuR suppressed spheroid formation and size, inhibited the expression of stemness-related marker, Oct4, Nanog and ALDH in lung canc...

journal_title：Biotechnology letters

authors： Zhang Y,Yang L,Ling C,Heng W

更新日期：2018-04-01 00:00:00

abstract：OBJECTS:To explore the roles of growth factor receptor-bound protein 14 (GRB14) in colorectal cancer (CRC) and its correlation with clinicopathological characteristics and prognosis of CRC patients. RESULTS:GRB14 was localized in the cytoplasm of CRC and benign glandular epithelium cells, showing higher levels in CRC ...

journal_title：Biotechnology letters

authors： Yang P,Wei J,Li W,He F,Zeng S,Zhang T,Sun Z,Cao J

更新日期：2016-06-01 00:00:00

abstract：:Aerobic granules for sulphide and ammonium removal were cultivated in a sequencing batch reactor, and the microbial community of the aerobic granules was investigated by denaturing gradient gel electrophoresis. The loading rate increased from 0.15 to 0.9 kg S2− m−3 d−1, and the removal efficiencies of sulphide, chemic...

journal_title：Biotechnology letters

authors： Su C,Zhu L,Zhang C,Qi X,Guo Y,Gao R

更新日期：2012-05-01 00:00:00

abstract：OBJECTIVES:Breast cancer is a popular fatal malignant tumor for women with high of rates incidence and mortality. Development of the new approaches for breast cancer targeted diagnosis and chemotherapy is emergently needed by the current clinical practice, the important first step is finding a breast cancer specificall...

journal_title：Biotechnology letters

authors： Jin H,Gao X,Xiao L,He H,Cheng S,Zhang C,Hou Y,Song F,Su X,Gao Q,Lu Z,Yang R,Song X,Yang J,Duan W,Hou Y

更新日期：2021-01-01 00:00:00

abstract：:A single-compartmented microbial fuel cell composed of a graphite felt anode modified with Neutral Red (NR-anode) and a porous Fe(II)-carbon cathode (FeC-cathode) were compared for electricity generation from Microbacterium sp. and Pseudomonas sp. under identical conditions. Pseudomonas sp. was more than four times th...

journal_title：Biotechnology letters

authors： Lee SW,Jeon BY,Park DH

更新日期：2010-04-01 00:00:00

abstract：:Quercetin was methylated with two O-methyltransferases (OMTs) expressed in E. coli. A construct (RSOMT) was designed to express two OMTs: ROMT-9, which methylates specifically at the 3'-hydroxyl group of quercetin and SOMT-2, which methylates at the 4'-hydroxyl group. Both OMT genes were driven by T7 promoters and had...

journal_title：Biotechnology letters

authors： Kim BG,Shin KH,Lee Y,Hur HG,Lim Y,Ahn JH

更新日期：2005-12-01 00:00:00

abstract：:Electro-acupuncture (EA) at Fenglong acupoint (ST40) can lower the levels of serum cholesterol and triacylglycerols. To study the hepatic genes responsible for the cholesterol-lowering effect of EA, suppression subtractive hybridization combined with the switch mechanism at the 5'-end of RNA template cDNA synthesis an...

journal_title：Biotechnology letters

authors： Li L,Tan GH,Zhang YZ

更新日期：2012-07-01 00:00:00

abstract：:GERI-155 is a macrolide antibiotic containing two deoxyhexose molecules which has antimicrobial activities against gram-positive bacteria. The deoxyhexose biosynthetic gene cluster of GERI-155 from Streptomyces sp. GERI-155 genome has now been isolated. Four orf were identified and a putative orf, supposed to code for...

journal_title：Biotechnology letters

authors： Sohng JK,Kim HJ,Nam DH,Lim DO,Han JM,Lee HJ,Yoo JC

更新日期：2004-02-01 00:00:00

abstract：:In vitro biocatalysis with cytochrome P450 BM-3 was investigated aiming for the substitution of the expensive natural cofactor NADPH by electrochemistry. The monooxygenase was immobilized on electrodes by entrapment in polypyrrole as a conductive polymer for electrochemically wiring the enzyme. Electropolymerization o...

journal_title：Biotechnology letters

authors： Holtmann D,Mangold KM,Schrader J

更新日期：2009-05-01 00:00:00

abstract：:An automated sequential injection analysis (SIA) system using stop-flow technique was developed to determine methanol concentration by means of the enzymatic reactions of alcohol oxidase and peroxidase. Its application as an on-line device for monitoring Pichia pastoris fermentations producing an heterologous protein ...

journal_title：Biotechnology letters

authors： Surribas A,Cos O,Montesinos JL,Valero F

更新日期：2003-11-01 00:00:00

abstract：OBJECTIVES:To develop a colorimetric assay for ATP based on the blue-pigment synthesising non-ribosomal peptide synthetase (NRPS) BpsA, and to demonstrate its utility in defining the substrate specificity of other NRPS enzymes. RESULTS:BpsA is able to convert two molecules of L-glutamine into the readily-detected blue...

journal_title：Biotechnology letters

authors： Brown AS,Calcott MJ,Collins VM,Owen JG,Ackerley DF

更新日期：2020-12-01 00:00:00

abstract：:Among single-site mutations of L-arabinose isomerase derived from Geobacillus thermodenitrificans, two mutants were produced having the lowest and highest activities of D-tagatose production. Site-directed mutagenesis at these sites showed that the aromatic ring at amino acid 164 and the size of amino acid 475 were im...

journal_title：Biotechnology letters

authors： Oh HJ,Kim HJ,Oh DK

更新日期：2006-02-01 00:00:00

abstract：OBJECTIVE:To improve the production of trans-10,cis-12-conjugated linoleic acid (t10,c12-CLA) from linoleic acid in recombinant Yarrowia lipolytica. RESULTS:Cells of the yeast were permeabilized by freeze/thawing. The optimal conditions for t10,c12-CLA production by the permeabilized cells were at 28 °C, pH 7, 200 rpm...

journal_title：Biotechnology letters

authors： Zhang B,Song Y,Chen H,Zhang H,Chen W

更新日期：2016-11-01 00:00:00

abstract：:Amberlite XAD 2 resin enhanced the asymmetric reduction of ethyl 4-chloroacetoacetate (ECA) to S-4-chloro-3-hydroxybutyric acid ethyl ester as catalyzed by Saccharomyces cerevisiae. The absorbed ECA was released slowly to the solution during the reaction so that the substrate inhibition and the spontaneous chemical hy...

journal_title：Biotechnology letters

authors： Houng JY,Liau JS

更新日期：2003-01-01 00:00:00

abstract：:In Klebsiella pneumoniae, aldehyde dehydrogenases (ALDH) convert 3-hydroxypropionaldehyde (3-HPA) into 3-hydroxypropionic acid (3-HP). Although ALDHs can increase the production of 3-HP in K. pneumoniae, the substrate specificity of ALDH homologues from other microorganisms toward 3-HPA is less documented. Here we rep...

journal_title：Biotechnology letters

authors： Su M,Li Y,Ge X,Tian P

更新日期：2015-03-01 00:00:00

abstract：:A coupled in silico thermodynamic and probabilistic metabolic control analysis methodology was verified by applying it to the glycerol biosynthetic pathway in Saccharomyces cerevisiae. The methodology allows predictions even when detailed knowledge of the enzyme kinetics is lacking. In a metabolic steady state, we fou...

journal_title：Biotechnology letters

authors： Birkenmeier M,Mack M,Röder T

更新日期：2015-02-01 00:00:00