Therapeutic potential of human umbilical cord blood mesenchymal stem cells on erectile function in rats with cavernous nerve injury.

abstract：:Treatment of a hyperthermophilic enzyme, alkaline phosphatase from Pyrococcus furiosus (PfuAP), with EDTA completely deactivated PfuAP, indicating that the presence of one or more divalent metal ions is essential for its catalytic activity. Subsequent addition of various divalent metal ions to the apoprotein recovered...

journal_title：Biotechnology letters

authors： Minamihata K,Goto M,Kamiya N

更新日期：2012-11-01 00:00:00

abstract：:The actinorhizal plant seabuckthorn (Hippophae rhamnoides L., Elaeagnaceae) is a wind pollinated dioecious crop. To distinguish male genotypes from female genotypes early in the vegetative growth phase, we have developed robust PCR-based marker(s). DNA bulk samples from 20 male and 20 female plants each were screened ...

journal_title：Biotechnology letters

authors： Korekar G,Sharma RK,Kumar R,Meenu,Bisht NC,Srivastava RB,Ahuja PS,Stobdan T

更新日期：2012-05-01 00:00:00

abstract：:An open reading frame (ORF) encoding chitin oligosaccharide deacetylase (Pa-COD) gene and its signal sequence was cloned from the Vibrio parahaemolyticus KN1699 genome and its sequence was analyzed. The ORF encoded a 427 amino acid protein, including the 22 amino acid signal sequence. The deduced amino acid sequence w...

journal_title：Biotechnology letters

authors： Kadokura K,Sakamoto Y,Saito K,Ikegami T,Hirano T,Hakamata W,Oku T,Nishio T

更新日期：2007-08-01 00:00:00

abstract：:A fusion protein consisting of streptavidin and firefly luciferase was constructed to establish an accurate measuring technique of local ATP concentration. The fusion protein retained the binding ability of streptavidin and enzymatic activity of luciferase. Also, it could detect the concentration of antigens and could...

journal_title：Biotechnology letters

authors： Nakamura M,Mie M,Funabashi H,Kobatake E

更新日期：2004-07-01 00:00:00

abstract：:5-Aminolevulinic acid (ALA) is an intermediate in the biosynthesis of tetrapyrroles. Its current production is expensive. We have developed a low-cost medium for Propionibacterium acidipropionici to produce extracellular ALA. When grown at 35 °C on a medium containing 3 % (w/v) food-grade sodium lactate supplemented w...

journal_title：Biotechnology letters

authors： Sonhom R,Thepsithar C,Jongsareejit B

更新日期：2012-09-01 00:00:00

abstract：:Two new strains of Escherichia coli B were engineered for the production of lactate with no detectable chiral impurity. All chiral impurities were eliminated by deleting the synthase gene (msgA) that converts dihydroxyacetone-phosphate to methylglyoxal, a precursor for both L: (+)- and D: (-)-lactate. Strain TG113 con...

journal_title：Biotechnology letters

authors： Grabar TB,Zhou S,Shanmugam KT,Yomano LP,Ingram LO

更新日期：2006-10-01 00:00:00

abstract：:Continuous production of fructooligosaccharides (FOS) by Aureobasidium pullulans immobilized on calcium alginate beads with a packed bed was investigated at a plant scale reactor. Optimum conditions were with 770 g sucrose/l, being fed at 200 l/h at 50°C which gave a productivity of 180 g FOS/l h. Initial activity was...

journal_title：Biotechnology letters

authors： Jung KH,Bang SH,Oh TK,Park HJ

更新日期：2011-08-01 00:00:00

abstract：OBJECTIVES:To prepare recombinant tPep-(vascular endothelial growth factor) VEGF-B and assess its biological activity. RESULTS:This new VEGF fusion protein was constructed using a targeting peptide and prepared using E.coli. The tPep-VEGF-B was refolded from inclusion bodies and purified using affinity chromatography....

journal_title：Biotechnology letters

authors： Zhang Y,Tong Y,Gao M,Luo C,Song X,Gao X,Yao W

更新日期：2016-07-01 00:00:00

abstract：:Arylomycins are type I signal peptidase inhibitors and have a potential as a new type of antibiotics. They were identified from the broth of Streptomyces sp. HCCB10043. The arylomycin biosynthetic gene cluster in this strain was identical to that in S. roseosporus. Within the gene cluster, aryC, encoding a P450 enzyme...

journal_title：Biotechnology letters

authors： Jin X,Rao M,Wei W,Ge M,Liu J,Chen D,Liang Y

更新日期：2012-12-01 00:00:00

abstract：:The stability (half-life, t(½)) of the large catalase (CAT) isolated from Aspergillus terreus was decreased under acidic conditions (maximum t(½) approximately 8.5 months at pH ≤ 6) versus alkaline conditions (t(½) approximately 15 months at pH 8-12). Acidic conditions induce the dissociation of haem from CAT, as reve...

journal_title：Biotechnology letters

authors： Vatsyayan P,Goswami P

更新日期：2011-02-01 00:00:00

abstract：:An efficient synthesis of polymerizable 3'- and 5'-O-acyl-nucleoside derivatives has been developed from inosine and 2'-deoxyuridine by enzyme-catalyzed regioselective acylation with divinyl dicarboxylates. In acetone, Lipozyme (immobilized lipase from Mucor miehei) gave 5'-O-acyl-nucleoside products, and PPL (lipase ...

journal_title：Biotechnology letters

authors： Sun XF,Wang N,Wu Q,Lin XF

更新日期：2004-06-01 00:00:00

abstract：OBJECTIVES:To screen the phylogenetically-nearest members of Cellulosimicrobium cellulans for the production of cellulosome-like multienzyme complexes and extracellular β-xylosidase activity against 7-xylosyltaxanes and to get corresponding molecular insights. RESULTS:Cellulosimicrobium (family Promicromonosporaceae) ...

journal_title：Biotechnology letters

authors： Wang W,Yu Y,Dou TY,Wang JY,Sun C

更新日期：2018-02-01 00:00:00

abstract：:Seeds play essential roles in plant life cycle and germination is a complex process which is associated with different phases of water imbibition. Upon imbibition, seeds begin utilization of storage substances coupled with metabolic activity and biosynthesis of new proteins. Regeneration of organelles and emergence of...

journal_title：Biotechnology letters

authors： Zaynab M,Kanwal S,Furqan M,Islam W,Noman A,Ali GM,Rehman N,Zafar S,Sughra K,Jahanzab M

更新日期：2017-10-01 00:00:00

abstract：:Eugenitin, a chromone derivative and a metabolite of the endophyte Mycoleptodiscus indicus, at 5 mM activated a recombinant GH11 endo-xylanase by 40 %. The in silico prediction of ligand-binding sites on the three-dimensional structure of the endo-xylanase revealed that eugenitin interacts mainly by a hydrogen bond wi...

journal_title：Biotechnology letters

authors： Andrioli WJ,Damásio AR,Silva TM,da Silva VB,Maller A,Nanayakkara NP,Silva CH,Polizeli ML,Bastos JK

更新日期：2012-08-01 00:00:00

abstract：:Non-viral vector, pCMV-Pth, with full-length human parathyroid hormone (prepro-hPTH) cDNA was constructed and delivered into the ovariectomized mouse quadriceps to explore the effects of electroporational gene transfer of PTH on the bone. The expression of hPTH in the transfected mice was detected by RT-PCR and radioi...

journal_title：Biotechnology letters

authors： Liu B,Tang J,Ji J,Gu J

更新日期：2003-12-01 00:00:00

abstract：:Glycosylation of the phenolic hydroxyl group of the phenyl propanoid systems, eugenol 1 and curcumin 2, using an amyloglucosidase from Rhizopus and a beta-glucosidase from sweet almonds together with carbohydrates (D-glucose 3, D-mannose 4, maltose 5, sucrose 6 and D-mannitol 7) in di-isopropyl ether produced glycosid...

journal_title：Biotechnology letters

authors： Vijayakumar GR,Divakar S

更新日期：2007-04-01 00:00:00

abstract：:Growth of Ruta graveolens shoots was induced when Bacillus sp. cell lysates were added to the culture medium. Elicitation of coumarin by this lysate was also very effective; the concentrations of isopimpinelin, xanthotoxin and bergapten increased to 610, 2120 and 1460 microg g(-1) dry wt, respectively. It also had a s...

journal_title：Biotechnology letters

authors： Orlita A,Sidwa-Gorycka M,Malinski E,Czerwicka M,Kumirska J,Golebiowski M,Lojkowska E,Stepnowski P

更新日期：2008-03-01 00:00:00

abstract：OBJECTIVES:To evaluate the potential of enzyme cocktails produced by five filamentous fungi to supplement the industrial cellulase cocktail, Celluclast 1.5L, in order to improve the efficiency of saccharification. RESULTS:The fungi were cultivated on wheat bran and the resulting supernatants were combined with Cellucl...

journal_title：Biotechnology letters

authors： Peciulyte A,Pisano M,de Vries RP,Olsson L

更新日期：2017-09-01 00:00:00

abstract：:Theabrownins (TB) are water-soluble phenolic compounds associated with the various health benefits of Pu-erh tea, a post-fermented Chinese dark tea. This work reports on the production of theabrownins from infusions of sun-dried green tea leaves using a pure culture of Aspergillus fumigatus isolated from a solid-state...

journal_title：Biotechnology letters

authors： Wang Q,Gong J,Chisti Y,Sirisansaneeyakul S

更新日期：2014-12-01 00:00:00

abstract：:Classical purification of the glycoprotein equine chorionic gonadotropin (eCG) from serum includes pH fractionation with metaphosphoric acid, two ethanol precipitation steps as well as dialysis followed by fixed-bed chromatography. A simplified process requiring only 1/3 of the solvent and improving the yield from 53 ...

journal_title：Biotechnology letters

authors： Müller C,Wagner K,Frankenfeld K,Franzreb M

更新日期：2011-05-01 00:00:00

abstract：:A domain of epiglucan was synthesized by beta-glucosidases. Two beta-glucosidases, an extracellular beta-glucosidase derived from Sclerotinia sclerotiorum grown on xylose, and a commercial lyophilized preparation of beta-glucosidase from Aspergillus niger, were used to synthesize gluco-oligosaccharides from cellobiose...

journal_title：Biotechnology letters

authors： Smaali MI,Michaud N,Marzouki N,Legoy MD,Maugard T

更新日期：2004-04-01 00:00:00

abstract：:Fungal diseases damage crop plants and affect agricultural production. Transgenic plants have been produced by inserting antifungal genes to confer resistance against fungal pathogens. Genes of fungal cell wall-degrading enzymes, such as chitinase and glucanase, are frequently used to produce fungal-resistant transgen...

journal_title：Biotechnology letters

authors： Ceasar SA,Ignacimuthu S

更新日期：2012-06-01 00:00:00

abstract：:Hydrophobin-fused domain III of dengue envelope proteins serotypes 1 and 2 were expressed in Rachiplusia nu larvae and purified by aqueous two-phase system. This biotechnological approach of hydrophobin-fused proteins, which allowed obtaining 97.7 µg/larva of fusion protein DomIII serotype 1 and 61.4 µg/larva of fusio...

journal_title：Biotechnology letters

authors： Cerezo J,Targovnik AM,Smith ME,González Maglio D,Luppo VC,Morales MA,Miranda MV,Rodríguez Talou J

更新日期：2020-03-01 00:00:00

abstract：OBJECTIVE:To produce (S)-3-hydroxy-1-(3-(trifluoromethyl)-5,6-dihydro[1,2,4]triazolo[4,3-a]pyrazin-7(8H)-yl]-4-(2,4,5-trifluorophenyl)butan-1-one (S)-1 from 4-oxo-4-[3-(trifluoromethyl)-5,6-dihydro [1,2,4]triazolo[4,3-a]pyrazin-7(8H)-yl)-1-(2,4,5-trifluorophenyl)butan-2-one (2) by microbial bioreduction. RESULTS:A new...

journal_title：Biotechnology letters

authors： Wei Y,Xia S,He C,Xiong W,Xu H

更新日期：2016-05-01 00:00:00

abstract：:A non-characterized gene, previously proposed as the D-tagatose-3-epimerase gene from Rhodobacter sphaeroides, was cloned and expressed in Escherichia coli. Its molecular mass was estimated to be 64 kDa with two identical subunits. The enzyme specificity was highest with D-fructose and decreased for other substrates i...

journal_title：Biotechnology letters

authors： Zhang L,Mu W,Jiang B,Zhang T

更新日期：2009-06-01 00:00:00

abstract：:Deer antlers are the only mammalian appendages capable of repeated rounds of regeneration. Every year, deer antlers are shed and regrown from blastema into large branched structures of cartilage and bone. Little is known about the genes involved in antler development particularly during the later stages of ossificatio...

journal_title：Biotechnology letters

authors： Yao B,Zhao Y,Zhang H,Zhang M,Liu M,Liu H,Li J

更新日期：2012-05-01 00:00:00

abstract：:The production of biodiesel by transesterification employing acid or base catalyst has been industrially accepted for its high conversion and reaction rates. Downstream processing costs and environmental problems associated with biodiesel production and byproducts recovery have led to the search for alternative produc...

journal_title：Biotechnology letters

authors： Bisen PS,Sanodiya BS,Thakur GS,Baghel RK,Prasad GB

更新日期：2010-08-01 00:00:00

abstract：:Fluctuating light intensity had a more significant impact on growth of gametophytes of transgenic Laminaria japonica in a 2500 ml bubble-column bioreactor than constant light intensity. A fluctuating light intensity between 10 and 110 microE m(-2) s(-1), with a photoperiod of 14 h:10 h light:dark, was the best regime ...

journal_title：Biotechnology letters

authors： Gao J,Zhang Y,Zhang W,Wu S,Qin S,Zhang W,Yu X

更新日期：2005-09-01 00:00:00

abstract：OBJECTIVES:Establish a method to indirectly measure evaporation in microwell-based cell culture systems and show that the proposed method allows compensating for liquid losses in fed-batch processes. RESULTS:A correlation between evaporation and the concentration of Na+ was found (R2 = 0.95) when using the 24-well-bas...

journal_title：Biotechnology letters

authors： Wiegmann V,Martinez CB,Baganz F

更新日期：2018-07-01 00:00:00

abstract：:A fully contained and efficient heterologous protein production system was designed using Brassica rapa rapa (turnip) hairy roots. Two expression cassettes containing a cauliflower mosaic virus (CaMV) 35S promoter with a duplicated enhancer region, an Arabidopsis thaliana sequence encoding a signal peptide and the CaM...

journal_title：Biotechnology letters

authors： Huet Y,Ekouna JP,Caron A,Mezreb K,Boitel-Conti M,Guerineau F

更新日期：2014-01-01 00:00:00