Improved cellobiose utilization in E. coli by including both hydrolysis and phosphorolysis mechanisms.

abstract：:Aptamers against inactive Vibrio alginolyticus were selected from an 82-nt ssDNA random library by systematic evolution of ligands by exponential enrichment. After 15 rounds of selection, the final pool of aptamers was highly specific for inactivated V. alginolyticus and had a dissociation constant of 27.5 ± 9.2 nM. U...

journal_title：Biotechnology letters

authors： Tang X,Zheng J,Yan Q,Li Z,Li Y

更新日期：2013-06-01 00:00:00

abstract：:A plasmid, pBGFP, carrying green fluorescent protein (gfp) and benomyl-resistance genes was constructed and transformed into Metarhizium anisopliae. The transformants grew normally and GFP fluorescence was detected. No change was found in virulence for the transformants. Fluorescence was detected in hyphae from the ha...

journal_title：Biotechnology letters

authors： Cao Y,Peng G,He Z,Wang Z,Yin Y,Xia Y

更新日期：2007-06-01 00:00:00

abstract：:Ochrobactrum anthropi cleaved the delta-(D-alpha-aminoadipoyl)-side chain from delta-(D-alpha-aminoadipoyl)-7-amino-4-methylcoumarin, a beta-lactamase-resistant cephalosporin C analogue. In whole cell conversions up to 1 nkat g(-1) dry cell wt were achieved. O. anthropi possesses also gamma-D-glutamyltranspeptidase ac...

journal_title：Biotechnology letters

authors： Patett F,Fischer L

更新日期：2005-12-01 00:00:00

abstract：:Angiogenin, a potent angiogenic factor, was cloned and expressed by Escherichia coli and then purified with gel filtration chromatography. Approximately 90% pure angiogenin was obtained to generate a monoclonal antibody. Using western immunoblotting and ELISA, we confirmed that monoclonal antibody C46 secreted from hy...

journal_title：Biotechnology letters

authors： Wu J,Zhang HB,Wang J,Yang TC,Xian J,Yang CH,Lai HW

更新日期：2008-06-01 00:00:00

abstract：:The old Annurca apple cultivar (Malus domestica), particularly appreciated for its peculiar flavor and crispy flesh, was studied in order to preserve its ancient germplasm. Twelve clones of Annurca were analyzed using random amplified polymorphic DNA (RAPD) and simple-sequence repeat (SSR) markers. Two out of 30 RAPD ...

journal_title：Biotechnology letters

authors： Iannaccone M,Palumbo D,Ventimiglia I,Patocchi A,Spigno P,Capparelli R

更新日期：2007-02-01 00:00:00

abstract：:Salt stress is considered one of the main abiotic factors to limit crop growth and productivity by affecting morpho-physiological and biochemical processes. Genetically, a number of salt tolerant Brassica varieties have been developed and introduced, but breeding of such varieties is time consuming. Therefore, current...

journal_title：Biotechnology letters

authors： Shah N,Anwar S,Xu J,Hou Z,Salah A,Khan S,Gong J,Shang Z,Qian L,Zhang C

更新日期：2018-08-01 00:00:00

abstract：:GERI-155 is a macrolide antibiotic containing two deoxyhexose molecules which has antimicrobial activities against gram-positive bacteria. The deoxyhexose biosynthetic gene cluster of GERI-155 from Streptomyces sp. GERI-155 genome has now been isolated. Four orf were identified and a putative orf, supposed to code for...

journal_title：Biotechnology letters

authors： Sohng JK,Kim HJ,Nam DH,Lim DO,Han JM,Lee HJ,Yoo JC

更新日期：2004-02-01 00:00:00

abstract：OBJECTIVE:Myelin oligodendrocyte glycoprotein (MOG) is one of the major autoantigens in multiple sclerosis (MS), therefore selective depletion of autoreactive lymphocytes exposing MOG-specific B cell receptors (BCRs) would be beneficial in terms of MS treatment. RESULTS:Using E. coli we generated an efficient protocol...

journal_title：Biotechnology letters

authors： Stepanov A,Belyy A,Kasheverov I,Rybinets A,Dronina M,Dyachenko I,Murashev A,Knorre V,Sakharov D,Ponomarenko N,Tsetlin V,Tonevitsky A,Deyev S,Belogurov A Jr,Gabibov A

更新日期：2016-07-01 00:00:00

abstract：OBJECTIVE:The effect of epicatechin-3-gallate (ECG), a polyphenol that is present in green tea, on doxorubicin (DOX) cytotoxicity in H9C2 cardiomyocytes and its underlying mechanisms were investigated. RESULTS:Pretreatment with ECG (20 and 30 μM) significantly increased DOX-induced apoptosis to 16-18% in H9C2 cardiomy...

journal_title：Biotechnology letters

authors： Cai Y,Hong HQ,Zhao L,Cai YP,Qin Y

更新日期：2015-10-01 00:00:00

abstract：:The ability of Aspergillus strains to utilize thin stillage to produce malic acid was compared. The highest malic acid was produced by Aspergillus niger ATCC 9142 at 17 g l(-1). Biomass production from thin stillage was similar with all strains but ATCC 10577 was the highest at 19 g l(-1). The highest malic acid yield...

journal_title：Biotechnology letters

authors： West TP

更新日期：2011-12-01 00:00:00

abstract：:C3H/HeJ mice were sensitized with ovalbumin (OVA) and choleratoxin (CT) for 5 weeks, and then Bifidobacterium bifidum BGN4 was administered continuously for 7 weeks, starting 2 weeks before (pre-treatment group) and 2 weeks after (post-treatment group) the initial sensitization. After sensitization, the OVA-induced (s...

journal_title：Biotechnology letters

authors： Kim H,Lee SY,Ji GE

更新日期：2005-09-01 00:00:00

abstract：:Fungal diseases damage crop plants and affect agricultural production. Transgenic plants have been produced by inserting antifungal genes to confer resistance against fungal pathogens. Genes of fungal cell wall-degrading enzymes, such as chitinase and glucanase, are frequently used to produce fungal-resistant transgen...

journal_title：Biotechnology letters

authors： Ceasar SA,Ignacimuthu S

更新日期：2012-06-01 00:00:00

abstract：:Cell adhesion to extracellular matrices, including fibronectin, results in clustering of integrins in focal adhesions. To promote the clustering of fibronectin and thus enhance its activity at the sites of focal adhesion formation, we have engineered a fusion protein containing recombinant fibronectin fragment (hFN) c...

journal_title：Biotechnology letters

authors： Chung CP,Jang JH

更新日期：2005-06-01 00:00:00

abstract：:Seventeen independent transgenic rice plants with the maize anthocyanin regulatory gene Lc under control of the CaMV 35S promoter were obtained and verified by molecular identification. Ten plants showed red spikelets during early development of florets, and the degenerate florets were still red after heading. Additio...

journal_title：Biotechnology letters

authors： Li Y,Zhang T,Shen ZW,Xu Y,Li JY

更新日期：2013-01-01 00:00:00

abstract：:L-threo-3,4-Dihydroxyphenylserine (DOPS) is a chiral unnatural beta-hydroxy amino acid used for the treatment of Parkinson disease. We developed a continuous bioconversion system for DOPS production that uses whole-cell biocatalyst of recombinant Escherichia coli expressing L-threonine aldolase (L-TA) genes cloned fro...

journal_title：Biotechnology letters

authors： Baik SH,Yoshioka H

更新日期：2009-03-01 00:00:00

abstract：:A dicistronic expression vector was constructed for Chinese hamster ovary (CHO) cells that produce both selectable marker-DHFR (dihydrofolate reductase) gene and recombinant antibody cDNA from a single primary transcript via differential splicing. The vector was derived from a pDHL vector and contained the human const...

journal_title：Biotechnology letters

authors： Xiong KH,Liang QC,Xiong H,Zou CX,Gao GD,Zhao ZW,Zhang H

更新日期：2005-11-01 00:00:00

abstract：OBJECTIVES:To acquire a thermostable xylanase, that is suitable for xylooligosaccharide production from pretreated corncobs, the metagenomic method was used to obtain the gene from an uncultured environmental microorganism. RESULTS:A thermostable xylanase-encoding gene (xyn10CD18) was cloned directly from the metageno...

journal_title：Biotechnology letters

authors： Sun MZ,Zheng HC,Meng LC,Sun JS,Song H,Bao YJ,Pei HS,Yan Z,Zhang XQ,Zhang JS,Liu YH,Lu FP

更新日期：2015-09-01 00:00:00

abstract：OBJECTIVES:To provide a simple method to make a stable ΔF508-CFTR-expressing T84 cell line that can be used as an efficient screening model system for ΔF508-CFTR rescue. RESULTS:CFTR knockout cell lines were generated by Cas9 with a single-guide RNA (sgRNA) targeting exon 1 of the CFTR genome, which produced indels th...

journal_title：Biotechnology letters

authors： Chung WY,Song M,Park J,Namkung W,Lee J,Kim H,Lee MG,Kim JY

更新日期：2016-12-01 00:00:00

abstract：:Virus-like particles (VLPs) are a promising and developing option for vaccination and gene therapy. They are also interesting as shuttles for drug targeting. Currently, several different gene expression systems are available, among which the silkworm expression system is known for its mass production capacity. However...

journal_title：Biotechnology letters

authors： Minkner R,Park EY

更新日期：2018-04-01 00:00:00

abstract：:Human beta-amyloid (Abeta) is believed to be one of the main components of Alzheimer's disease, so reduction of Abeta is considered a key therapeutic target. Using Agrobacterium-mediated nuclear transformation, we generated transgenic tomatoes for Abeta with tandem repeats. Integration of the human Abeta gene into the...

journal_title：Biotechnology letters

authors： Youm JW,Jeon JH,Kim H,Kim YH,Ko K,Joung H,Kim H

更新日期：2008-10-01 00:00:00

abstract：:The production of biodiesel by transesterification employing acid or base catalyst has been industrially accepted for its high conversion and reaction rates. Downstream processing costs and environmental problems associated with biodiesel production and byproducts recovery have led to the search for alternative produc...

journal_title：Biotechnology letters

authors： Bisen PS,Sanodiya BS,Thakur GS,Baghel RK,Prasad GB

更新日期：2010-08-01 00:00:00

abstract：:The use of microRNAs (miRNAs) for improving the efficiency of recombinant protein production by CHO cells is gaining considerable interest for their ability to regulate entire molecular networks. Differential miRNA expression profiling and large-scale transient screening have been the prerequisite for the selection of...

journal_title：Biotechnology letters

authors： Kelly PS,Gallagher C,Clynes M,Barron N

更新日期：2015-04-01 00:00:00

abstract：:Brevibacterium epidermis DSM 20659 is a halotolerant Gram-positive bacterium which can synthesize the osmolyte, ectoine, but prefers to take it up from its environment. The present study revealed that B. epidermis is equipped with at least one transport system for ectoine, with a maximal transport velocity of 15.7 +/-...

journal_title：Biotechnology letters

authors： Onraedt A,De Mey M,Walcarius B,Soetaert W,Vandamme EJ

更新日期：2006-11-01 00:00:00

abstract：:Most animal cell culture media can be buffered using bicarbonate and high pressure CO(2) in a closed system. However, in an open system, the pH of the culture media increases continuously due to the marked difference in CO(2) pressure between the culture media and the atmosphere. Therefore, it is important to measure ...

journal_title：Biotechnology letters

authors： Jang J,Moon SJ,Hong SH,Kim IH

更新日期：2010-11-01 00:00:00

abstract：OBJECTIVES:The action modes of an endocellulase, EGA, and its domains (CD9 and CBM3) during enzymatic treatment of cotton fabrics were investigated. RESULTS:EGA, CD9 and CBM3 had the binding capacity to cellulose substrates, of which the filter paper was the substrate with the strongest binding capacity. Analyses of s...

journal_title：Biotechnology letters

authors： Yu M,Qiu Y,Chen W,Zhao F,Shao J

更新日期：2015-08-01 00:00:00

abstract：:Dibutyl phthalate (DBP) was more efficiently degraded by cutinase compared to yeast esterase; i.e. almost 80% of initial DBP (500 mg l(-1)) was decomposed within 7.5 h, and nearly 50% of the degraded DBP disappeared within the initial 30 min. The toxicity of the final DBP degradation products were investigated using v...

journal_title：Biotechnology letters

authors： Kim YH,Lee J

更新日期：2005-05-01 00:00:00

abstract：:Streptomyces is an attractive host for heterologous protein secretion. To further optimize its expression capacity, better expression vectors will be helpful. Here, based on pSGL1, a high copy number plasmid present in Streptomyces globisporus C-1027, we constructed a series of novel E. coli-Streptomyces shuttle expre...

journal_title：Biotechnology letters

authors： Zhu Y,Wang L,Du Y,Wang S,Yu T,Hong B

更新日期：2011-02-01 00:00:00

abstract：OBJECTIVES:A potent HIV vaccine should overcome some limitations such as polymorphism of human HLA, the diversity of HIV-1 virus, and the lack of an effective delivery system. In this study, a DNA construct encoding Nef60-84, Nef126-144, Vpr34-47, Vpr60-75, Gp16030-53, Gp160308-323, and P248-151 epitopes was designed u...

journal_title：Biotechnology letters

authors： Davoodi S,Bolhassani A,Namazi F

更新日期：2021-01-01 00:00:00

abstract：:A sigma 70-like gene, sigA, has been identified from Corynebacterium ammoniagenes. The sigA gene encodes a polypeptide of 467 amino acids with a calculated molecular mass of 52036 Da. The deduced amino acid sequence preserves the common motifs of the primary sigma factors and shows very high similarity to those of Sig...

journal_title：Biotechnology letters

authors： Lee BR,Kim JH

更新日期：2004-04-01 00:00:00

abstract：:Some pest management programs employ PCR to identify cry1 genes from Bacillus thuringiensis to predict bacterial toxicity towards different insect pests. However, due to changes on the mode of action of the Cry proteins, new primers had to be designed to detect the new genes. Therefore, an 'in-silico' study of genetic...

journal_title：Biotechnology letters

authors： Davolos CC,Guidelli-Thuler AM,de Abreu IL,Sena JA,Lemos MV

更新日期：2009-12-01 00:00:00